This content is the Seurat PBMC tutorial with an additional section to add in Monocle for pseudotime trajectory analysis.

Download the raw data

Unzip it in the terminal with:

tar -xvf pbmc3k_filtered_gene_bc_matrices.tar.gz

Setup the Seurat Object

For this tutorial, we will be analyzing the a dataset of Peripheral Blood Mononuclear Cells (PBMC) freely available from 10X Genomics. There are 2,700 single cells that were sequenced on the Illumina NextSeq 500. The raw data can be found here.

We start by reading in the data. The Read10X() function reads in the output of the cellranger pipeline from 10X, returning a unique molecular identified (UMI) count matrix. The values in this matrix represent the number of molecules for each feature (i.e. gene; row) that are detected in each cell (column).

We next use the count matrix to create a Seurat object. The object serves as a container that contains both data (like the count matrix) and analysis (like PCA, or clustering results) for a single-cell dataset. For a technical discussion of the Seurat object structure, check out our GitHub Wiki. For example, the count matrix is stored in pbmc[["RNA"]]@counts.

Discussion on the Seurat Object in R

Class

These are essentially data containers in R as a class, and can accessed as a variable in the R environment.

Classes are pre-defined and can contain multiple data tables and metadata. For Seurat, there are three types.

  • Seurat - the main data class, contains all the data.
  • Assay - found within the Seurat object. RNA, ATAC
  • DimReduc - for PCA and UMAP

Slots

Slots are parts within a class that contain specific data. These can be lists, data tables and vectors.

Data Access

Many of the functions in Seurat operate on the data class and slots within them seamlessly, there maybe occasion to acess these separately to hack them however, this is an advanced analysis method.

The ways to access the slots can be through methods for the class (functions) or with standard R accessor nomenclature.

library(dplyr)
library(ggplot2)
library(Seurat)
library(patchwork)

# Load the PBMC dataset
pbmc.data <- Read10X(data.dir = "../data/pbmc3k/filtered_gene_bc_matrices/hg19/")  ## Please change this line to wherever you have unzipped the raw data

# Initialize the Seurat object with the raw (non-normalized data).
pbmc <- CreateSeuratObject(counts = pbmc.data, project = "pbmc3k", min.cells = 3, min.features = 200)
pbmc
## An object of class Seurat 
## 13714 features across 2700 samples within 1 assay 
## Active assay: RNA (13714 features, 0 variable features)
What does data in a count matrix look like? 
# Lets examine a few genes in the first thirty cells
pbmc.data[c("CD3D", "TCL1A", "MS4A1"), 1:30]
## 3 x 30 sparse Matrix of class "dgCMatrix"
##                                                                    
## CD3D  4 . 10 . . 1 2 3 1 . . 2 7 1 . . 1 3 . 2  3 . . . . . 3 4 1 5
## TCL1A . .  . . . . . . 1 . . . . . . . . . . .  . 1 . . . . . . . .
## MS4A1 . 6  . . . . . . 1 1 1 . . . . . . . . . 36 1 2 . . 2 . . . .

The . values in the matrix represent 0s (no molecules detected). Since most values in an scRNA-seq matrix are 0, Seurat uses a sparse-matrix representation whenever possible. This results in significant memory and speed savings for Drop-seq/inDrop/10x data.

dense.size <- object.size(as.matrix(pbmc.data))
dense.size
## 709591472 bytes
sparse.size <- object.size(pbmc.data)
sparse.size
## 29905192 bytes
dense.size/sparse.size
## 23.7 bytes

Side Bar Challenge

The count matrix represents all the cells in the sample (columns) and the genes (rows), why would most count values of genes in cells be zero?


Standard pre-processing workflow

The steps below encompass the standard pre-processing workflow for scRNA-seq data in Seurat. These represent the selection and filtration of cells based on QC metrics, data normalization and scaling, and the detection of highly variable features.

QC and selecting cells for further analysis

Seurat allows you to easily explore QC metrics and filter cells based on any user-defined criteria. A few QC metrics commonly used by the community include

  • The number of unique genes detected in each cell.
    • Low-quality cells or empty droplets will often have very few genes
    • Cell doublets or multiplets may exhibit an aberrantly high gene count
  • Similarly, the total number of molecules detected within a cell (correlates strongly with unique genes)
  • The percentage of reads that map to the mitochondrial genome
    • Low-quality / dying cells often exhibit extensive mitochondrial contamination
    • We calculate mitochondrial QC metrics with the PercentageFeatureSet() function, which calculates the percentage of counts originating from a set of features
    • We use the set of all genes starting with MT- as a set of mitochondrial genes
# The [[ operator can add columns to object metadata. This is a great place to stash QC stats
pbmc[["percent.mt"]] <- PercentageFeatureSet(pbmc, pattern = "^MT-")
Where are QC metrics stored in Seurat?
  • The number of unique genes and total molecules are automatically calculated during CreateSeuratObject()
    • You can find them stored in the object meta data
# Show QC metrics for the first 5 cells
head(pbmc@meta.data, 5)
orig.ident nCount_RNA nFeature_RNA percent.mt
AAACATACAACCAC-1 pbmc3k 2419 779 3.0177759
AAACATTGAGCTAC-1 pbmc3k 4903 1352 3.7935958
AAACATTGATCAGC-1 pbmc3k 3147 1129 0.8897363
AAACCGTGCTTCCG-1 pbmc3k 2639 960 1.7430845
AAACCGTGTATGCG-1 pbmc3k 980 521 1.2244898

 

In the example below, we visualize QC metrics, and use these to filter cells.

  • We filter cells that have unique feature counts over 2,500 or less than 200
  • We filter cells that have >5% mitochondrial counts
# Visualize QC metrics as a violin plot
VlnPlot(pbmc, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)

# FeatureScatter is typically used to visualize feature-feature relationships, but can be used
# for anything calculated by the object, i.e. columns in object metadata, PC scores etc.

plot1 <- FeatureScatter(pbmc, feature1 = "nCount_RNA", feature2 = "percent.mt")
plot2 <- FeatureScatter(pbmc, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
plot1 + plot2

Challenge Side Bar - What are the appropriate thresholds to use to remove poor QC cells?

Are the thresholds we set, reasonable? What happens if we relax the thresholds? Lets plot the percent mt with number of features.

plot3 <- FeatureScatter(pbmc, feature1 = "nFeature_RNA", feature2 = "percent.mt")
plot3

Okay we are happy with our thresholds, lets apply them and subset our data. This will remove the cells we think are of poor quality.

pbmc <- subset(pbmc, subset = nFeature_RNA > 200 & nFeature_RNA < 2500 & percent.mt < 5)
Challenge Side Bar - Feature Plots after subsetting

Lets replot the feature scatters and see what they look like.

plot5 <- FeatureScatter(pbmc, feature1 = "nCount_RNA", feature2 = "percent.mt")
plot6 <- FeatureScatter(pbmc, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")

plot1 + plot5

plot2 + plot6

Normalizing the data

After removing unwanted cells from the dataset, the next step is to normalize the data. By default, we employ a global-scaling normalization method “LogNormalize” that normalizes the feature expression measurements for each cell by the total expression, multiplies this by a scale factor (10,000 by default), and log-transforms the result. Normalized values are stored in pbmc[["RNA"]]@data.

pbmc <- NormalizeData(pbmc, normalization.method = "LogNormalize", scale.factor = 10000)

For clarity, in this previous line of code (and in future commands), we provide the default values for certain parameters in the function call. However, this isn’t required and the same behavior can be achieved with:

pbmc <- NormalizeData(pbmc)

Identification of highly variable features (feature selection)

We next calculate a subset of features that exhibit high cell-to-cell variation in the dataset (i.e, they are highly expressed in some cells, and lowly expressed in others). We and others have found that focusing on these genes in downstream analysis helps to highlight biological signal in single-cell datasets.

Our procedure in Seurat is described in detail here, and improves on previous versions by directly modeling the mean-variance relationship inherent in single-cell data, and is implemented in the FindVariableFeatures() function. By default, we return 2,000 features per dataset. These will be used in downstream analysis, like PCA.

pbmc <- FindVariableFeatures(pbmc, selection.method = "vst", nfeatures = 2000)

# Identify the 10 most highly variable genes
top10 <- head(VariableFeatures(pbmc), 10)

# plot variable features with and without labels
plot1 <- VariableFeaturePlot(pbmc)
plot2 <- LabelPoints(plot = plot1, points = top10, repel = TRUE)
plot1 + plot2

Challenge Side Bar - Labelling Genes of Interest

What if we wanted to see more genes of interest? What can we do? Increase the number of top genes.

# Identify the 10 most highly variable genes
top20 <- head(VariableFeatures(pbmc), 20)

# plot variable features with and without labels
plot3 <- LabelPoints(plot = plot1, points = top20, repel = TRUE)
plot2 + plot3

Scaling the data

Next, we apply a linear transformation (‘scaling’) that is a standard pre-processing step prior to dimensional reduction techniques like PCA. The ScaleData() function:

all.genes <- rownames(pbmc)
pbmc <- ScaleData(pbmc, features = all.genes)
This step takes too long! Can I make it faster?

Scaling is an essential step in the Seurat workflow, but only on genes that will be used as input to PCA. Therefore, the default in ScaleData() is only to perform scaling on the previously identified variable features (2,000 by default). To do this, omit the features argument in the previous function call, i.e.

pbmc <- ScaleData(pbmc)
Your PCA and clustering results will be unaffected. However, Seurat heatmaps (produced as shown below with DoHeatmap()) require genes in the heatmap to be scaled, to make sure highly-expressed genes don’t dominate the heatmap. To make sure we don’t leave any genes out of the heatmap later, we are scaling all genes in this tutorial.
 
How can I remove unwanted sources of variation, as in Seurat v2?

In Seurat v2 we also use the ScaleData() function to remove unwanted sources of variation from a single-cell dataset. For example, we could ‘regress out’ heterogeneity associated with (for example) cell cycle stage, or mitochondrial contamination. These features are still supported in ScaleData() in Seurat v3, i.e.:

pbmc <- ScaleData(pbmc, vars.to.regress = "percent.mt")
However, particularly for advanced users who would like to use this functionality, we strongly recommend the use of our new normalization workflow, SCTransform(). The method is described in our paper, with a separate vignette using Seurat v3 here. As with ScaleData(), the function SCTransform() also includes a vars.to.regress parameter.

 

Perform linear dimensional reduction

Next we perform PCA on the scaled data. By default, only the previously determined variable features are used as input, but can be defined using features argument if you wish to choose a different subset.

pbmc <- RunPCA(pbmc, features = VariableFeatures(object = pbmc))

Seurat provides several useful ways of visualizing both cells and features that define the PCA, including VizDimReduction(), DimPlot(), and DimHeatmap()

# Examine and visualize PCA results a few different ways
print(pbmc[["pca"]], dims = 1:5, nfeatures = 5)
## PC_ 1 
## Positive:  CST3, TYROBP, LST1, AIF1, FTL 
## Negative:  MALAT1, LTB, IL32, IL7R, CD2 
## PC_ 2 
## Positive:  CD79A, MS4A1, TCL1A, HLA-DQA1, HLA-DQB1 
## Negative:  NKG7, PRF1, CST7, GZMB, GZMA 
## PC_ 3 
## Positive:  HLA-DQA1, CD79A, CD79B, HLA-DQB1, HLA-DPB1 
## Negative:  PPBP, PF4, SDPR, SPARC, GNG11 
## PC_ 4 
## Positive:  HLA-DQA1, CD79B, CD79A, MS4A1, HLA-DQB1 
## Negative:  VIM, IL7R, S100A6, IL32, S100A8 
## PC_ 5 
## Positive:  GZMB, NKG7, S100A8, FGFBP2, GNLY 
## Negative:  LTB, IL7R, CKB, VIM, MS4A7
VizDimLoadings(pbmc, dims = 1:2, reduction = "pca")

DimPlot(pbmc, reduction = "pca")

In particular DimHeatmap() allows for easy exploration of the primary sources of heterogeneity in a dataset, and can be useful when trying to decide which PCs to include for further downstream analyses. Both cells and features are ordered according to their PCA scores. Setting cells to a number plots the ‘extreme’ cells on both ends of the spectrum, which dramatically speeds plotting for large datasets. Though clearly a supervised analysis, we find this to be a valuable tool for exploring correlated feature sets.

DimHeatmap(pbmc, dims = 1, cells = 500, balanced = TRUE)

DimHeatmap(pbmc, dims = 1:15, cells = 500, balanced = TRUE)

Determine the ‘dimensionality’ of the dataset

To overcome the extensive technical noise in any single feature for scRNA-seq data, Seurat clusters cells based on their PCA scores, with each PC essentially representing a ‘metafeature’ that combines information across a correlated feature set. The top principal components therefore represent a robust compression of the dataset. However, how many components should we choose to include? 10? 20? 100?

In Macosko et al, we implemented a resampling test inspired by the JackStraw procedure. We randomly permute a subset of the data (1% by default) and rerun PCA, constructing a ‘null distribution’ of feature scores, and repeat this procedure. We identify ‘significant’ PCs as those who have a strong enrichment of low p-value features.

# Note: The Seurat defaults are num.replicates=100, prop.freq=0.01.  The parameters we're
# using here are just for speed, and will give conservative p-values.

pbmc <- JackStraw(pbmc, num.replicate = 10, prop.freq = 0.1)
pbmc <- ScoreJackStraw(pbmc, dims = 1:20)

The JackStrawPlot() function provides a visualization tool for comparing the distribution of p-values for each PC with a uniform distribution (dashed line). ‘Significant’ PCs will show a strong enrichment of features with low p-values (solid curve above the dashed line). In this case it appears that there is a sharp drop-off in significance after the first 10-12 PCs.

JackStrawPlot(pbmc, dims = 1:15)

# Seurat plots this in a weird way. Let's adjust it a bit.
JackStrawPlot(pbmc, dims = 1:15) + coord_cartesian() + geom_abline(intercept = 0, slope = 0.05)

# For each PC, the furthest point to the right below the solid line gives proportion of
# significant genes with FDR 0.05.

An alternative heuristic method generates an ‘Elbow plot’: a ranking of principle components based on the percentage of variance explained by each one (ElbowPlot() function). In this example, we can observe an ‘elbow’ around PC9-10, suggesting that the majority of true signal is captured in the first 10 PCs.

ElbowPlot(pbmc)

Identifying the true dimensionality of a dataset – can be challenging/uncertain for the user. We therefore suggest these three approaches to consider. The first is more supervised, exploring PCs to determine relevant sources of heterogeneity, and could be used in conjunction with GSEA for example. The second implements a statistical test based on a random null model, but is time-consuming for large datasets, and may not return a clear PC cutoff. The third is a heuristic that is commonly used, and can be calculated instantly. In this example, all three approaches yielded similar results, but we might have been justified in choosing anything between PC 7-12 as a cutoff.

We chose 10 here, but encourage users to consider the following:

Cluster the cells

Seurat v3 applies a graph-based clustering approach, building upon initial strategies in (Macosko et al). Importantly, the distance metric which drives the clustering analysis (based on previously identified PCs) remains the same. However, our approach to partitioning the cellular distance matrix into clusters has dramatically improved. Our approach was heavily inspired by recent manuscripts which applied graph-based clustering approaches to scRNA-seq data [SNN-Cliq, Xu and Su, Bioinformatics, 2015] and CyTOF data [PhenoGraph, Levine et al., Cell, 2015]. Briefly, these methods embed cells in a graph structure - for example a K-nearest neighbor (KNN) graph, with edges drawn between cells with similar feature expression patterns, and then attempt to partition this graph into highly interconnected ‘quasi-cliques’ or ‘communities’.

As in PhenoGraph, we first construct a KNN graph based on the euclidean distance in PCA space, and refine the edge weights between any two cells based on the shared overlap in their local neighborhoods (Jaccard similarity). This step is performed using the FindNeighbors() function, and takes as input the previously defined dimensionality of the dataset (first 10 PCs).

To cluster the cells, we next apply modularity optimization techniques such as the Louvain algorithm (default) or SLM [SLM, Blondel et al., Journal of Statistical Mechanics], to iteratively group cells together, with the goal of optimizing the standard modularity function. The FindClusters() function implements this procedure, and contains a resolution parameter that sets the ‘granularity’ of the downstream clustering, with increased values leading to a greater number of clusters. We find that setting this parameter between 0.4-1.2 typically returns good results for single-cell datasets of around 3K cells. Optimal resolution often increases for larger datasets. The clusters can be found using the Idents() function.

pbmc <- FindNeighbors(pbmc, dims = 1:10)
pbmc <- FindClusters(pbmc, resolution = 0.5)
## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
## 
## Number of nodes: 2638
## Number of edges: 95965
## 
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.8723
## Number of communities: 9
## Elapsed time: 0 seconds
# Look at cluster IDs of the first 5 cells
head(Idents(pbmc), 5)
## AAACATACAACCAC-1 AAACATTGAGCTAC-1 AAACATTGATCAGC-1 AAACCGTGCTTCCG-1 
##                2                3                2                1 
## AAACCGTGTATGCG-1 
##                6 
## Levels: 0 1 2 3 4 5 6 7 8

Run non-linear dimensional reduction (UMAP/tSNE)

Seurat offers several non-linear dimensional reduction techniques, such as tSNE and UMAP, to visualize and explore these datasets. The goal of these algorithms is to learn the underlying manifold of the data in order to place similar cells together in low-dimensional space. Cells within the graph-based clusters determined above should co-localize on these dimension reduction plots. As input to the UMAP and tSNE, we suggest using the same PCs as input to the clustering analysis.

# If you haven't installed UMAP, you can do so via reticulate::py_install(packages =
# 'umap-learn')
pbmc <- RunUMAP(pbmc, dims = 1:10)
# note that you can set `label = TRUE` or use the LabelClusters function to help label
# individual clusters
DimPlot(pbmc, reduction = "umap")

You can save the object at this point so that it can easily be loaded back in without having to rerun the computationally intensive steps performed above, or easily shared with collaborators.

saveRDS(pbmc, file = "../output/pbmc_tutorial.rds")

Finding differentially expressed features (cluster biomarkers)

Seurat can help you find markers that define clusters via differential expression. By default, it identifies positive and negative markers of a single cluster (specified in ident.1), compared to all other cells. FindAllMarkers() automates this process for all clusters, but you can also test groups of clusters vs. each other, or against all cells.

The min.pct argument requires a feature to be detected at a minimum percentage in either of the two groups of cells, and the thresh.test argument requires a feature to be differentially expressed (on average) by some amount between the two groups. You can set both of these to 0, but with a dramatic increase in time - since this will test a large number of features that are unlikely to be highly discriminatory. As another option to speed up these computations, max.cells.per.ident can be set. This will downsample each identity class to have no more cells than whatever this is set to. While there is generally going to be a loss in power, the speed increases can be significant and the most highly differentially expressed features will likely still rise to the top.

# find all markers of cluster 2
cluster2.markers <- FindMarkers(pbmc, ident.1 = 2, min.pct = 0.25)
head(cluster2.markers, n = 5)
p_val avg_log2FC pct.1 pct.2 p_val_adj
IL32 0 1.2154360 0.949 0.466 0
LTB 0 1.2828597 0.981 0.644 0
CD3D 0 0.9359210 0.922 0.433 0
IL7R 0 1.1776027 0.748 0.327 0
LDHB 0 0.8837324 0.953 0.614 0 
# find all markers distinguishing cluster 5 from clusters 0 and 3
cluster5.markers <- FindMarkers(pbmc, ident.1 = 5, ident.2 = c(0, 3), min.pct = 0.25)
head(cluster5.markers, n = 5)
p_val avg_log2FC pct.1 pct.2 p_val_adj
FCGR3A 0 4.267579 0.975 0.039 0
IFITM3 0 3.877105 0.975 0.048 0
CFD 0 3.411039 0.938 0.037 0
CD68 0 3.014535 0.926 0.035 0
RP11-290F20.3 0 2.722684 0.840 0.016 0 
# find markers for every cluster compared to all remaining cells, report only the positive
# ones
pbmc.markers <- FindAllMarkers(pbmc, only.pos = TRUE, min.pct = 0.25, logfc.threshold = 0.25)
pbmc.markers %>%
    group_by(cluster) %>%
    slice_max(n = 2, order_by = avg_log2FC)
p_val avg_log2FC pct.1 pct.2 p_val_adj cluster gene
0 1.333503 0.435 0.108 0 0 CCR7
0 1.069166 0.897 0.593 0 0 LDHB
0 5.570063 0.996 0.215 0 1 S100A9
0 5.477394 0.975 0.121 0 1 S100A8
0 1.282860 0.981 0.644 0 2 LTB
0 1.240361 0.424 0.111 0 2 AQP3
0 4.310172 0.936 0.041 0 3 CD79A
0 3.591579 0.622 0.022 0 3 TCL1A
0 3.006740 0.595 0.056 0 4 GZMK
0 2.966206 0.957 0.241 0 4 CCL5
0 3.311697 0.975 0.134 0 5 FCGR3A
0 3.085654 1.000 0.315 0 5 LST1
0 4.917370 0.958 0.135 0 6 GNLY
0 4.888172 0.986 0.071 0 6 GZMB
0 3.871151 0.812 0.011 0 7 FCER1A
0 2.874465 1.000 0.513 0 7 HLA-DPB1
0 8.575862 1.000 0.024 0 8 PPBP
0 7.243377 1.000 0.010 0 8 PF4

Seurat has several tests for differential expression which can be set with the test.use parameter (see our DE vignette for details). For example, the ROC test returns the ‘classification power’ for any individual marker (ranging from 0 - random, to 1 - perfect).

cluster0.markers <- FindMarkers(pbmc, ident.1 = 0, logfc.threshold = 0.25, test.use = "roc", only.pos = TRUE)

We include several tools for visualizing marker expression. VlnPlot() (shows expression probability distributions across clusters), and FeaturePlot() (visualizes feature expression on a tSNE or PCA plot) are our most commonly used visualizations. We also suggest exploring RidgePlot(), CellScatter(), and DotPlot() as additional methods to view your dataset.

VlnPlot(pbmc, features = c("MS4A1", "CD79A"))

# you can plot raw counts as well
VlnPlot(pbmc, features = c("NKG7", "PF4"), slot = "counts", log = TRUE)

FeaturePlot(pbmc, features = c("MS4A1", "GNLY", "CD3E", "CD14", "FCER1A", "FCGR3A", "LYZ", "PPBP",
    "CD8A"))
Other useful plots

These are ridgeplots, cell scatter plots and dotplots. Replace FeaturePlot with the other functions.

RidgePlot(pbmc, features = c("MS4A1", "GNLY", "CD3E", "CD14", "FCER1A", "FCGR3A", "LYZ", "PPBP",
    "CD8A"))

For CellScatter plots, will need the cell id of the cells you want to look at. You could access this using the [[ notation.

pbmc[[]]
orig.ident nCount_RNA nFeature_RNA percent.mt RNA_snn_res.0.5 seurat_clusters
AAACATACAACCAC-1 pbmc3k 2419 779 3.0177759 2 2
AAACATTGAGCTAC-1 pbmc3k 4903 1352 3.7935958 3 3
AAACATTGATCAGC-1 pbmc3k 3147 1129 0.8897363 2 2
AAACCGTGCTTCCG-1 pbmc3k 2639 960 1.7430845 1 1
AAACCGTGTATGCG-1 pbmc3k 980 521 1.2244898 6 6
AAACGCACTGGTAC-1 pbmc3k 2163 781 1.6643551 2 2
AAACGCTGACCAGT-1 pbmc3k 2175 782 3.8160920 4 4
AAACGCTGGTTCTT-1 pbmc3k 2260 790 3.0973451 4 4
AAACGCTGTAGCCA-1 pbmc3k 1275 532 1.1764706 0 0
AAACGCTGTTTCTG-1 pbmc3k 1103 550 2.9011786 5 5
AAACTTGAAAAACG-1 pbmc3k 3914 1112 2.6315789 3 3
AAACTTGATCCAGA-1 pbmc3k 2388 747 1.0887772 0 0
AAAGAGACGAGATA-1 pbmc3k 2410 864 1.0788382 0 0
AAAGAGACGCGAGA-1 pbmc3k 3033 1058 1.4177382 1 1
AAAGAGACGGACTT-1 pbmc3k 1151 457 2.3457863 0 0
AAAGAGACGGCATT-1 pbmc3k 792 335 2.3989899 0 0
AAAGCAGATATCGG-1 pbmc3k 4584 1422 1.3961606 1 1
AAAGCCTGTATGCG-1 pbmc3k 2928 1013 1.7076503 2 2
AAAGGCCTGTCTAG-1 pbmc3k 4973 1445 1.5282526 3 3
AAAGTTTGATCACG-1 pbmc3k 1268 444 3.4700315 3 3
AAAGTTTGGGGTGA-1 pbmc3k 3281 1015 2.5906736 3 3
AAAGTTTGTAGAGA-1 pbmc3k 1102 417 1.5426497 0 0
AAAGTTTGTAGCGT-1 pbmc3k 2683 877 2.4972046 1 1
AAATCAACAATGCC-1 pbmc3k 2319 787 1.1642950 3 3
AAATCAACACCAGT-1 pbmc3k 1412 508 1.9830028 0 0
AAATCAACCAGGAG-1 pbmc3k 2800 823 2.2500000 0 0
AAATCAACCCTATT-1 pbmc3k 5676 1541 2.4312896 5 5
AAATCAACGGAAGC-1 pbmc3k 3473 996 1.7564066 0 0
AAATCAACTCGCAA-1 pbmc3k 2811 936 1.8498755 2 2
AAATCATGACCACA-1 pbmc3k 4128 1368 4.5784884 5 5
AAATCCCTCCACAA-1 pbmc3k 955 427 1.9895288 0 0
AAATCCCTGCTATG-1 pbmc3k 822 406 1.7031630 3 3
AAATGTTGAACGAA-1 pbmc3k 3208 1017 1.9326683 1 1
AAATGTTGCCACAA-1 pbmc3k 1760 785 0.6250000 2 2
AAATGTTGTGGCAT-1 pbmc3k 2761 1017 1.9195943 1 1
AAATTCGAAGGTTC-1 pbmc3k 2740 749 2.2262774 0 0
AAATTCGAATCACG-1 pbmc3k 2567 822 1.9867550 1 1
AAATTCGAGCTGAT-1 pbmc3k 2969 980 2.1892893 5 5
AAATTCGAGGAGTG-1 pbmc3k 2978 873 2.4848892 0 0
AAATTCGATTCTCA-1 pbmc3k 2641 928 1.4388489 4 4
AAATTGACACGACT-1 pbmc3k 2357 836 2.1213407 4 4
AAATTGACTCGCTC-1 pbmc3k 3411 1013 1.4951627 2 2
AACAAACTCATTTC-1 pbmc3k 2178 731 1.4233242 0 0
AACAAACTTTCGTT-1 pbmc3k 2688 877 1.7113095 0 0
AACAATACGACGAG-1 pbmc3k 2104 782 2.9942966 0 0
AACACGTGCAGAGG-1 pbmc3k 1799 785 0.8893830 2 2
AACACGTGGAAAGT-1 pbmc3k 2629 788 1.0270065 0 0
AACACGTGGAACCT-1 pbmc3k 2280 880 2.2368421 1 1
AACACGTGGCTACA-1 pbmc3k 2652 800 2.4132730 0 0
AACACGTGTACGAC-1 pbmc3k 3569 1213 2.7458672 5 5
AACAGCACAAGAGT-1 pbmc3k 688 342 2.4709302 1 1
AACATTGATGGGAG-1 pbmc3k 4711 1458 3.1628104 5 5
AACCAGTGATACCG-1 pbmc3k 3840 1249 2.7864583 5 5
AACCCAGATCGCTC-1 pbmc3k 2343 756 1.1096884 0 0
AACCGATGCTCCCA-1 pbmc3k 2294 835 3.6617262 0 0
AACCGATGGTCATG-1 pbmc3k 2632 823 2.0896657 3 3
AACCGATGTTCTAC-1 pbmc3k 2294 825 2.5719268 1 1
AACCGCCTAGCGTT-1 pbmc3k 3390 1237 1.6519174 2 2
AACCGCCTCTACGA-1 pbmc3k 3994 1240 3.4802203 1 1
AACCTACTGTGAGG-1 pbmc3k 5682 1649 1.5839493 1 1
AACCTACTGTGTTG-1 pbmc3k 2675 843 2.6542056 0 0
AACCTTACGAGACG-1 pbmc3k 2771 825 2.5261638 0 0
AACCTTACGCGAGA-1 pbmc3k 1246 654 4.7351525 6 6
AACCTTACTAACGC-1 pbmc3k 1947 776 1.6949153 5 5
AACCTTTGGACGGA-1 pbmc3k 2076 764 1.3005780 0 0
AACCTTTGTACGCA-1 pbmc3k 4617 1461 1.9709768 5 5
AACGCAACAAGTAG-1 pbmc3k 2048 789 1.5136719 2 2
AACGCATGACCCAA-1 pbmc3k 2774 868 2.1989906 0 0
AACGCATGCCTTCG-1 pbmc3k 2866 803 2.6866713 0 0
AACGCATGTACTTC-1 pbmc3k 2913 963 1.4074837 0 0
AACGCCCTCGGGAA-1 pbmc3k 2502 799 2.1982414 2 2
AACGCCCTCGTACA-1 pbmc3k 1915 876 1.4099217 6 6
AACGCCCTGGCATT-1 pbmc3k 1442 690 2.7739251 2 2
AACGTCGAGTATCG-1 pbmc3k 2148 987 2.0018622 6 6
AACGTGTGAAAGCA-1 pbmc3k 2820 906 2.4468085 0 0
AACGTGTGGCGGAA-1 pbmc3k 2070 740 2.0772947 3 3
AACGTGTGTCCAAG-1 pbmc3k 1665 620 2.4624625 0 0
AACGTGTGTGCTTT-1 pbmc3k 1932 866 1.2422360 2 2
AACTACCTTAGAGA-1 pbmc3k 2874 914 1.4961726 0 0
AACTCACTCAAGCT-1 pbmc3k 2605 967 3.1477927 1 1
AACTCACTTGGAGG-1 pbmc3k 2122 801 1.5080113 1 1
AACTCGGAAAGTGA-1 pbmc3k 1840 732 3.6413043 4 4
AACTCGGAAGGTCT-1 pbmc3k 1289 554 2.4049651 1 1
AACTCTTGCAGGAG-1 pbmc3k 2102 789 0.9990485 2 2
AACTGTCTCCCTTG-1 pbmc3k 2326 857 2.1926053 1 1
AACTTGCTACGCTA-1 pbmc3k 2335 899 2.6552463 3 3
AACTTGCTGGGACA-1 pbmc3k 1864 674 2.1995708 0 0
AAGAACGAGTGTTG-1 pbmc3k 780 397 1.4102564 0 0
AAGAAGACGTAGGG-1 pbmc3k 1437 660 2.0876827 3 3
AAGACAGAAGTCTG-1 pbmc3k 1125 563 1.8666667 3 3
AAGACAGAGGATCT-1 pbmc3k 2314 785 1.2532411 0 0
AAGACAGATTACCT-1 pbmc3k 2309 859 2.4686011 1 1
AAGAGATGGGTAGG-1 pbmc3k 1345 567 2.0817844 3 3
AAGATGGAAAACAG-1 pbmc3k 878 412 2.5056948 3 3
AAGATGGAGAACTC-1 pbmc3k 3116 1042 1.7008986 1 1
AAGATGGAGATAAG-1 pbmc3k 4019 1202 3.9313262 1 1
AAGATTACAACCTG-1 pbmc3k 2117 700 1.7005196 0 0
AAGATTACAGATCC-1 pbmc3k 4058 1256 1.4785609 1 1
AAGATTACCCGTTC-1 pbmc3k 2762 928 2.5706010 1 1
AAGATTACCGCCTT-1 pbmc3k 3062 1078 1.7635532 7 7
AAGATTACCTCAAG-1 pbmc3k 2279 937 2.4572181 6 6
AAGATTACTCCTCG-1 pbmc3k 642 316 2.6479751 1 1
AAGCAAGAGCGAGA-1 pbmc3k 2478 898 2.3002421 2 2
AAGCAAGAGCTTAG-1 pbmc3k 2168 916 2.2140221 6 6
AAGCAAGAGGTGTT-1 pbmc3k 1684 857 1.7220903 6 6
AAGCACTGAGCAAA-1 pbmc3k 3235 899 2.7202473 3 3
AAGCACTGCATACG-1 pbmc3k 803 390 0.1245330 0 0
AAGCACTGGTTCTT-1 pbmc3k 6153 1713 2.3565740 3 3
AAGCCAACGTGTTG-1 pbmc3k 2764 819 1.4471780 0 0
AAGCCATGAACTGC-1 pbmc3k 7064 1871 1.4014723 7 7
AAGCCATGACACGT-1 pbmc3k 1535 657 1.4983713 1 1
AAGCCATGCGTGAT-1 pbmc3k 2110 790 1.0900474 0 0
AAGCCATGTCTCGC-1 pbmc3k 908 477 3.5242291 0 0
AAGCCTGACATGCA-1 pbmc3k 1789 768 1.7887088 2 2
AAGCCTGACCGAAT-1 pbmc3k 1047 480 2.0057307 1 1
AAGCGACTCCTCAC-1 pbmc3k 2102 818 1.7126546 5 5
AAGCGACTGTGTCA-1 pbmc3k 3131 866 1.5969339 0 0
AAGCGACTTACAGC-1 pbmc3k 1494 600 1.8072289 3 3
AAGCGACTTTGACG-1 pbmc3k 3668 1185 1.5267176 5 5
AAGCGTACGTCTTT-1 pbmc3k 2046 648 3.9100684 0 0
AAGGCTTGCGAACT-1 pbmc3k 2075 774 0.9156627 4 4
AAGGTCACGGTTAC-1 pbmc3k 1870 699 2.4598930 1 1
AAGGTCACTGTTTC-1 pbmc3k 1773 640 1.8612521 3 3
AAGGTCACTTCCCG-1 pbmc3k 2385 855 2.4737945 4 4
AAGGTCTGACAGTC-1 pbmc3k 2587 830 3.9041361 2 2
AAGGTCTGCAGATC-1 pbmc3k 967 387 0.8273009 0 0
AAGTAACTCTGAAC-1 pbmc3k 1874 710 2.1878335 1 1
AAGTAACTGAGATA-1 pbmc3k 659 341 0.6069803 3 3
AAGTAGGATACAGC-1 pbmc3k 1723 892 1.8572258 6 6
AAGTATACCGAACT-1 pbmc3k 3251 933 1.8455860 0 0
AAGTCCGACTTGTT-1 pbmc3k 1523 604 2.4294156 0 0
AAGTCCGATAGAAG-1 pbmc3k 3368 1007 1.8408551 0 0
AAGTCTCTAGTCGT-1 pbmc3k 3282 980 2.3765996 0 0
AAGTCTCTCGGAGA-1 pbmc3k 1838 679 3.0467900 4 4
AAGTGGCTTGGAGG-1 pbmc3k 1572 603 2.0356234 3 3
AAGTTCCTCATTCT-1 pbmc3k 2334 862 1.9708655 5 5
AAGTTCCTTCTTAC-1 pbmc3k 3264 1030 2.9411765 2 2
AATAAGCTCGAATC-1 pbmc3k 2759 886 2.3559261 2 2
AATAAGCTCGTTGA-1 pbmc3k 1604 601 1.3092269 0 0
AATACCCTGGACGA-1 pbmc3k 1500 609 2.2000000 3 3
AATACCCTGGCATT-1 pbmc3k 3309 1118 1.5714717 2 2
AATACTGAAAGGGC-1 pbmc3k 1745 667 1.0315186 4 4
AATACTGAATTGGC-1 pbmc3k 1815 793 1.9283747 6 6
AATAGGGAACCCTC-1 pbmc3k 2874 960 1.5657620 3 3
AATAGGGAGAATGA-1 pbmc3k 1861 753 3.6002149 4 4
AATCAAACTATCGG-1 pbmc3k 1457 637 2.2649279 4 4
AATCCGGAATGCTG-1 pbmc3k 2804 1031 1.4978602 3 3
AATCCTACCGGTAT-1 pbmc3k 2440 776 2.5409836 0 0
AATCCTTGACGGGA-1 pbmc3k 2533 860 2.3687327 1 1
AATCCTTGGTGAGG-1 pbmc3k 1753 824 1.2549914 6 6
AATCGGTGGAACTC-1 pbmc3k 1953 850 2.1505376 4 4
AATCGGTGTGCTTT-1 pbmc3k 1894 743 1.9535375 0 0
AATCTAGAAAAGTG-1 pbmc3k 2581 857 1.9759783 2 2
AATCTAGAATCGGT-1 pbmc3k 3166 923 2.2109918 0 0
AATCTCACAGCCTA-1 pbmc3k 3553 1228 2.1390374 1 1
AATCTCACTCTAGG-1 pbmc3k 2147 804 0.8383791 0 0
AATCTCTGAACAGA-1 pbmc3k 2183 715 1.6032982 0 0
AATCTCTGCTTTAC-1 pbmc3k 1709 667 1.8139263 6 6
AATGATACACCAAC-1 pbmc3k 1918 779 0.7299270 4 4
AATGATACGGTCAT-1 pbmc3k 3092 1194 2.0051746 1 1
AATGCGTGACACCA-1 pbmc3k 2866 888 1.6050244 3 3
AATGCGTGGACGGA-1 pbmc3k 4432 1266 2.1209386 1 1
AATGCGTGGCTATG-1 pbmc3k 2224 872 2.0683453 3 3
AATGGAGAATCGTG-1 pbmc3k 2380 846 3.2773109 1 1
AATGGAGATCCTTA-1 pbmc3k 1958 780 2.5536261 4 4
AATGGCTGACACCA-1 pbmc3k 3037 958 2.3707606 3 3
AATGGCTGCGTGAT-1 pbmc3k 2209 803 1.9918515 2 2
AATGGCTGTAAAGG-1 pbmc3k 1286 554 4.8211509 4 4
AATGGCTGTACTCT-1 pbmc3k 1207 603 4.9710025 4 4
AATGGCTGTGAAGA-1 pbmc3k 2142 782 2.6610644 0 0
AATGTAACGGTGGA-1 pbmc3k 3047 978 0.5579258 0 0
AATGTCCTCTTCTA-1 pbmc3k 3321 909 1.6561277 2 2
AATGTTGACAGTCA-1 pbmc3k 2738 935 1.9722425 2 2
AATGTTGAGTTGAC-1 pbmc3k 2995 996 1.5025042 1 1
AATGTTGATCTACT-1 pbmc3k 2672 960 1.7215569 2 2
AATTACGAATTCCT-1 pbmc3k 4510 1313 1.8847007 7 7
AATTACGACTTCTA-1 pbmc3k 2568 798 1.1682243 0 0
AATTACGAGTGAGG-1 pbmc3k 3465 1110 0.9235209 2 2
AATTACGATTGGCA-1 pbmc3k 1730 676 3.5838150 3 3
AATTCCTGCTCAGA-1 pbmc3k 1799 774 2.3902168 3 3
AATTGATGTCGCAA-1 pbmc3k 3970 1295 3.0982368 5 5
AATTGTGACTTGGA-1 pbmc3k 1972 656 1.7241379 3 3
ACAAAGGAGGGTGA-1 pbmc3k 2017 624 2.4789291 0 0
ACAAATTGATTCTC-1 pbmc3k 4094 1313 2.6380068 5 5
ACAAATTGCTCAGA-1 pbmc3k 954 466 2.8301887 1 1
ACAAATTGTTGCGA-1 pbmc3k 2326 936 2.6225279 6 6
ACAACCGAGGGATG-1 pbmc3k 2384 977 1.7617450 6 6
ACAACCGAGTTACG-1 pbmc3k 2104 778 1.8060837 2 2
ACAAGAGAAGTCGT-1 pbmc3k 4144 1310 3.1853282 1 1
ACAAGAGACTTATC-1 pbmc3k 947 431 4.4350581 0 0
ACAAGAGAGTTGAC-1 pbmc3k 1622 577 4.4389642 3 3
ACAATCCTAACCGT-1 pbmc3k 2316 849 1.4680484 2 2
ACAATCCTTAGCGT-1 pbmc3k 1845 734 3.4688347 3 3
ACAATTGACTGACA-1 pbmc3k 2163 798 2.1729080 4 4
ACAATTGATGACTG-1 pbmc3k 1869 890 1.8726592 6 6
ACACAGACCATACG-1 pbmc3k 2021 857 2.2266205 4 4
ACACCAGAGGGCAA-1 pbmc3k 1768 719 1.3009050 4 4
ACACCCTGGTGTTG-1 pbmc3k 1806 820 1.3842746 6 6
ACACGAACAGTTCG-1 pbmc3k 1523 680 1.5758372 2 2
ACACGATGACGCAT-1 pbmc3k 2057 951 2.7710258 4 4
ACACGATGATGTGC-1 pbmc3k 2314 889 3.4572169 1 1
ACACGATGTCGTAG-1 pbmc3k 3651 1264 1.9172829 4 4
ACACGATGTGGTCA-1 pbmc3k 2310 918 1.6883117 4 4
ACAGACACGGCATT-1 pbmc3k 2137 797 3.1352363 2 2
ACAGACACGTTGTG-1 pbmc3k 1981 830 1.0095911 2 2
ACAGCAACACCTAG-1 pbmc3k 1046 496 1.8164436 1 1
ACAGCAACCTCAAG-1 pbmc3k 4431 1472 2.0311442 5 5
ACAGGTACCCCACT-1 pbmc3k 2727 846 1.0267693 0 0
ACAGGTACGCTGTA-1 pbmc3k 2219 868 3.1545741 4 4
ACAGGTACTGGTGT-1 pbmc3k 1885 1058 1.2732095 6 6
ACAGTCGACCCAAA-1 pbmc3k 1008 488 2.2817460 0 0
ACAGTCGACCGATA-1 pbmc3k 2068 895 1.0154739 4 4
ACAGTGACTCACCC-1 pbmc3k 2161 831 1.1568718 4 4
ACAGTGACTCTATC-1 pbmc3k 2131 864 4.1295167 4 4
ACAGTGTGGTCACA-1 pbmc3k 963 418 2.2845275 0 0
ACAGTGTGTTGCGA-1 pbmc3k 2273 855 2.2437308 1 1
ACATCACTCTACTT-1 pbmc3k 2502 906 1.9184652 1 1
ACATGGTGAAGCCT-1 pbmc3k 2165 791 1.2009238 0 0
ACATGGTGCAACCA-1 pbmc3k 2147 756 2.7480205 3 3
ACATGGTGCGAGTT-1 pbmc3k 2046 769 1.1730205 0 0
ACATTCTGGCATAC-1 pbmc3k 2848 955 1.6151685 0 0
ACATTCTGGGAACG-1 pbmc3k 3715 1189 1.6689098 1 1
ACCAACGACATGCA-1 pbmc3k 2114 679 1.4191107 0 0
ACCACAGAAAGTAG-1 pbmc3k 1457 524 3.2258065 0 0
ACCACAGAGTTGGT-1 pbmc3k 2856 904 1.2254902 0 0
ACCACCTGTGTGCA-1 pbmc3k 1176 505 1.7006803 0 0
ACCACGCTACAGCT-1 pbmc3k 2530 849 1.6205534 0 0
ACCACGCTACCCAA-1 pbmc3k 1936 775 2.4793388 4 4
ACCACGCTGCGAGA-1 pbmc3k 1979 793 2.3749368 0 0
ACCACGCTGCTGTA-1 pbmc3k 2008 747 3.5856574 0 0
ACCAGCCTGACAGG-1 pbmc3k 2569 951 1.4791748 2 2
ACCAGTGAACGGTT-1 pbmc3k 1730 643 3.3526012 3 3
ACCAGTGAATACCG-1 pbmc3k 3869 1275 3.2049625 5 5
ACCAGTGAGGGATG-1 pbmc3k 2444 827 0.9001637 0 0
ACCAGTGATGACTG-1 pbmc3k 1223 480 2.7800491 1 1
ACCATTACCTTCTA-1 pbmc3k 3415 969 1.9033675 0 0
ACCATTACGAGATA-1 pbmc3k 2709 1109 0.7751938 2 2
ACCATTTGTCATTC-1 pbmc3k 1647 648 2.0036430 0 0
ACCCAAGAACTGTG-1 pbmc3k 1704 805 2.1126761 4 4
ACCCAAGAATTCCT-1 pbmc3k 3562 1222 1.4879281 5 5
ACCCAAGAGGACAG-1 pbmc3k 2975 1022 1.1092437 2 2
ACCCAAGATTCACT-1 pbmc3k 1973 666 2.8890015 3 3
ACCCACTGCGCCTT-1 pbmc3k 986 489 2.7383367 1 1
ACCCACTGGACAGG-1 pbmc3k 863 390 2.4333720 0 0
ACCCACTGGTTCAG-1 pbmc3k 556 338 1.2589928 8 8
ACCCACTGTCGTAG-1 pbmc3k 2872 1111 3.1337047 1 1
ACCCAGCTCAGAAA-1 pbmc3k 2388 836 1.8844221 2 2
ACCCAGCTGTTAGC-1 pbmc3k 5534 1546 2.0599928 1 1
ACCCAGCTTGCTTT-1 pbmc3k 2495 840 0.4408818 0 0
ACCCGTTGATGACC-1 pbmc3k 1508 581 2.9177719 3 3
ACCCGTTGCTGCAA-1 pbmc3k 2157 747 0.7417710 0 0
ACCCGTTGCTTCTA-1 pbmc3k 6083 1852 1.4302154 7 7
ACCCTCGACCTATT-1 pbmc3k 1727 735 2.2003474 2 2
ACCCTCGACGGTAT-1 pbmc3k 1244 487 1.8488746 3 3
ACCCTCGATAAGGA-1 pbmc3k 2845 1014 2.6362039 1 1
ACCCTCGATCAAGC-1 pbmc3k 1443 584 2.8413028 5 5
ACCGTGCTACCAGT-1 pbmc3k 1936 795 1.5495868 2 2
ACCGTGCTGGAACG-1 pbmc3k 1908 768 2.2012579 3 3
ACCTATTGCTGAGT-1 pbmc3k 1190 490 2.6050420 3 3
ACCTATTGTGCCCT-1 pbmc3k 4056 1303 2.7613412 5 5
ACCTCCGAGTCCTC-1 pbmc3k 2425 894 1.0721649 2 2
ACCTCCGATATGCG-1 pbmc3k 1789 686 2.5712689 0 0
ACCTCCGATGCTGA-1 pbmc3k 1788 601 2.2930649 0 0
ACCTCGTGAACCAC-1 pbmc3k 1968 771 2.9979675 4 4
ACCTGAGATATCGG-1 pbmc3k 1523 703 3.7426133 8 8
ACCTGGCTAAGTAG-1 pbmc3k 1869 891 2.8892456 6 6
ACCTTTGACTCCCA-1 pbmc3k 3055 1168 2.2258592 1 1
ACCTTTGAGGAACG-1 pbmc3k 4144 1374 3.3059846 1 1
ACCTTTGAGGAAGC-1 pbmc3k 3695 1188 1.7050068 1 1
ACGAACACCTTGTT-1 pbmc3k 1734 890 1.3264129 6 6
ACGAACTGGCTATG-1 pbmc3k 8875 2413 1.5774648 8 8
ACGAAGCTCTCCAC-1 pbmc3k 795 354 1.0062893 0 0
ACGAAGCTCTGAGT-1 pbmc3k 7062 1852 3.3418295 3 3
ACGACCCTATCTCT-1 pbmc3k 4076 1316 2.0608440 5 5
ACGACCCTGATGAA-1 pbmc3k 2011 700 3.0333168 0 0
ACGACCCTTGACAC-1 pbmc3k 2793 823 1.6469746 3 3
ACGACCCTTGACCA-1 pbmc3k 2314 736 2.7225583 0 0
ACGAGGGACAGGAG-1 pbmc3k 7928 1991 1.4631685 7 7
ACGAGGGACGAACT-1 pbmc3k 2758 888 2.5018129 0 0
ACGAGGGATGTAGC-1 pbmc3k 3052 1033 2.3918742 2 2
ACGAGTACCCTAAG-1 pbmc3k 1257 543 1.8297534 3 3
ACGAGTACGAATCC-1 pbmc3k 3619 1186 1.5750207 3 3
ACGATCGAGGACTT-1 pbmc3k 1604 656 0.9351621 4 4
ACGATCGAGTCACA-1 pbmc3k 3065 1055 1.9902121 1 1
ACGATGACAATGCC-1 pbmc3k 2245 818 2.0489978 2 2
ACGATGACTGGTCA-1 pbmc3k 2632 977 1.8237082 2 2
ACGATTCTACGGGA-1 pbmc3k 1606 632 3.4246575 0 0
ACGCAATGGTTCAG-1 pbmc3k 1227 598 2.6079870 6 6
ACGCACCTGTTAGC-1 pbmc3k 1687 690 2.1339656 3 3
ACGCCACTGAACTC-1 pbmc3k 602 310 0.0000000 1 1
ACGCCGGAAACCAC-1 pbmc3k 2243 803 2.2737405 1 1
ACGCCGGAAAGCCT-1 pbmc3k 2143 741 0.8866076 0 0
ACGCCGGAAATGCC-1 pbmc3k 1102 560 2.2686025 2 2
ACGCCTTGCTCCCA-1 pbmc3k 2772 1071 3.0303030 5 5
ACGCGGTGGCGAGA-1 pbmc3k 2432 844 2.0559211 2 2
ACGCGGTGTGTGGT-1 pbmc3k 2603 881 2.2666154 3 3
ACGCGGTGTTTGCT-1 pbmc3k 2144 840 1.6324627 4 4
ACGCTCACAGTACC-1 pbmc3k 2367 815 2.7038445 0 0
ACGCTCACCCTTGC-1 pbmc3k 2162 768 2.3126735 0 0
ACGCTGCTGTTCTT-1 pbmc3k 1954 974 1.0747185 6 6
ACGGAACTCAGATC-1 pbmc3k 2154 985 1.8570102 6 6
ACGGAACTGTCGTA-1 pbmc3k 2151 818 3.9051604 5 5
ACGGAGGACTCTTA-1 pbmc3k 2613 956 2.8702641 1 1
ACGGATTGGGAGGT-1 pbmc3k 1654 639 2.7811366 3 3
ACGGATTGGTTAGC-1 pbmc3k 3068 1012 1.8252934 2 2
ACGGCTCTGAGCAG-1 pbmc3k 2388 926 2.5125628 2 2
ACGGCTCTTGCACA-1 pbmc3k 2535 794 1.8934911 0 0
ACGGTAACCGCTAA-1 pbmc3k 1865 751 2.6809651 4 4
ACGGTAACCTTCGC-1 pbmc3k 1944 791 2.3148148 4 4
ACGGTAACGGTGGA-1 pbmc3k 810 365 4.1975309 0 0
ACGGTAACTCGCAA-1 pbmc3k 1213 539 2.2258862 4 4
ACGGTATGAGTCGT-1 pbmc3k 1764 752 2.4376417 1 1
ACGGTATGGGTATC-1 pbmc3k 2149 769 3.8157282 3 3
ACGGTATGGTTGTG-1 pbmc3k 1946 649 2.2610483 3 3
ACGGTCCTAACGGG-1 pbmc3k 2196 946 1.3661202 2 2
ACGGTCCTCGGGAA-1 pbmc3k 2254 771 2.3513753 0 0
ACGTAGACAACCAC-1 pbmc3k 2027 823 0.9866798 2 2
ACGTAGACTACAGC-1 pbmc3k 1828 743 1.7505470 3 3
ACGTCAGAAACGAA-1 pbmc3k 2166 836 2.1698984 2 2
ACGTCAGAGAGCTT-1 pbmc3k 1909 721 2.4096386 1 1
ACGTCAGAGGGATG-1 pbmc3k 1541 640 1.4925373 0 0
ACGTCCTGATAAGG-1 pbmc3k 2627 923 3.5020936 1 1
ACGTCCTGTGAACC-1 pbmc3k 3301 1172 2.4235080 1 1
ACGTCGCTCCTGAA-1 pbmc3k 3924 1589 1.8603466 4 4
ACGTCGCTCTATTC-1 pbmc3k 1584 698 3.4090909 1 1
ACGTCGCTTCTCAT-1 pbmc3k 1600 616 2.5000000 3 3
ACGTGATGCCATGA-1 pbmc3k 5437 1414 2.6301269 7 7
ACGTGATGGGTCTA-1 pbmc3k 2633 815 1.8989746 0 0
ACGTGATGTAACCG-1 pbmc3k 2974 1045 1.5467384 5 5
ACGTGATGTGACAC-1 pbmc3k 2134 980 3.1865042 6 6
ACGTGCCTCCGTAA-1 pbmc3k 2176 865 1.3786765 2 2
ACGTGCCTTCTATC-1 pbmc3k 1080 526 3.7037037 4 4
ACGTTACTTTCCAT-1 pbmc3k 1967 761 1.6268429 0 0
ACGTTGGAAAAGCA-1 pbmc3k 2187 717 1.9204390 0 0
ACGTTGGAAACCTG-1 pbmc3k 2217 860 4.0144339 4 4
ACGTTGGACCGTAA-1 pbmc3k 1382 602 2.4602026 1 1
ACGTTGGAGCCAAT-1 pbmc3k 1780 828 2.2471910 6 6
ACGTTGGATATGGC-1 pbmc3k 1051 475 1.2369172 3 3
ACGTTGGATCAGGT-1 pbmc3k 2799 1069 1.5005359 1 1
ACGTTTACATCAGC-1 pbmc3k 2367 774 1.5631601 0 0
ACTAAAACCCACAA-1 pbmc3k 1267 613 1.4206788 0 0
ACTAAAACTCGACA-1 pbmc3k 2580 909 2.0930233 1 1
ACTACGGAATTTCC-1 pbmc3k 2187 835 1.9204390 2 2
ACTACGGACCTATT-1 pbmc3k 1804 669 2.6607539 0 0
ACTACGGATCGCTC-1 pbmc3k 2471 942 1.3759611 0 0
ACTACTACTAAGGA-1 pbmc3k 2339 792 2.3941855 0 0
ACTAGGTGGAACCT-1 pbmc3k 1980 871 1.3636364 4 4
ACTAGGTGGAACTC-1 pbmc3k 3528 1043 1.7006803 2 2
ACTATCACCTTGGA-1 pbmc3k 2048 857 3.0761719 4 4
ACTATCACTGCCAA-1 pbmc3k 2222 793 1.6651665 0 0
ACTCAGGACTGAAC-1 pbmc3k 2159 821 0.5558129 0 0
ACTCAGGATCTATC-1 pbmc3k 1869 750 1.4981273 3 3
ACTCAGGATTCGTT-1 pbmc3k 1009 435 1.9821606 1 1
ACTCCTCTCAACTG-1 pbmc3k 3266 1141 2.0820576 2 2
ACTCGCACGAAAGT-1 pbmc3k 1822 779 1.6465423 1 1
ACTCGCACTACGAC-1 pbmc3k 1872 718 1.1217949 1 1
ACTCTCCTGACACT-1 pbmc3k 1236 470 2.9126214 0 0
ACTCTCCTGCATAC-1 pbmc3k 5850 1739 1.7435897 0 0
ACTCTCCTGTTTGG-1 pbmc3k 2751 888 1.9265722 0 0
ACTGAGACAACCAC-1 pbmc3k 1942 839 3.5530381 4 4
ACTGAGACCCATAG-1 pbmc3k 3632 1153 1.7896476 5 5
ACTGAGACGTTGGT-1 pbmc3k 3989 1029 1.7548258 3 3
ACTGCCACACACGT-1 pbmc3k 2120 912 1.6037736 4 4
ACTGCCACTCCGTC-1 pbmc3k 1737 872 4.0875072 6 6
ACTGGCCTTCAGTG-1 pbmc3k 1656 824 2.0531401 6 6
ACTGTGGACGTGTA-1 pbmc3k 3183 1231 2.1049325 4 4
ACTGTGGATCTAGG-1 pbmc3k 4410 1310 2.2902494 3 3
ACTGTTACCCACAA-1 pbmc3k 1261 574 3.2513878 1 1
ACTGTTACTGCAGT-1 pbmc3k 1658 605 2.2919180 0 0
ACTTAAGAACCACA-1 pbmc3k 2296 716 2.2648084 0 0
ACTTAAGATTACTC-1 pbmc3k 2821 1018 2.8713222 7 7
ACTTAGCTGCGTAT-1 pbmc3k 3264 1213 1.5625000 5 5
ACTTAGCTGGGAGT-1 pbmc3k 2501 927 0.9196321 2 2
ACTTCAACAAGCAA-1 pbmc3k 5416 1777 3.0834564 2 2
ACTTCAACGTAGGG-1 pbmc3k 1256 654 1.1146497 6 6
ACTTCCCTTTCCGC-1 pbmc3k 2072 718 1.3030888 0 0
ACTTCTGACATGCA-1 pbmc3k 1655 646 4.3504532 3 3
ACTTGACTCCACAA-1 pbmc3k 1994 803 1.6048144 3 3
ACTTGGGAGAAAGT-1 pbmc3k 3623 1119 2.8981507 1 1
ACTTGGGAGGTTTG-1 pbmc3k 1644 749 1.7639903 2 2
ACTTGGGATGTGAC-1 pbmc3k 791 389 0.1264223 5 5
ACTTGGGATTGACG-1 pbmc3k 3108 1097 2.3487773 1 1
ACTTGTACCTGTCC-1 pbmc3k 1148 455 1.2195122 0 0
ACTTTGTGGAAAGT-1 pbmc3k 2368 755 1.1402027 0 0
ACTTTGTGGATAGA-1 pbmc3k 3143 1116 1.3999364 1 1
AGAAACGAAAGTAG-1 pbmc3k 1428 569 2.8711485 3 3
AGAAAGTGCGCAAT-1 pbmc3k 3021 867 2.0191989 0 0
AGAAAGTGGGGATG-1 pbmc3k 1966 726 1.6276704 1 1
AGAACAGAAATGCC-1 pbmc3k 1879 916 1.7030335 6 6
AGAACAGACGACTA-1 pbmc3k 980 490 2.5510204 3 3
AGAACAGAGACAAA-1 pbmc3k 3002 960 1.7321785 2 2
AGAACGCTTTGCTT-1 pbmc3k 1762 624 2.1566402 0 0
AGAAGATGTGACTG-1 pbmc3k 3602 1088 0.9994448 2 2
AGAATGGAAGAAGT-1 pbmc3k 2051 771 1.0238908 4 4
AGAATTTGTAACCG-1 pbmc3k 3257 966 1.0746085 0 0
AGAATTTGTAGAGA-1 pbmc3k 1426 478 4.1374474 0 0
AGACACACTGTAGC-1 pbmc3k 2227 808 1.3022003 0 0
AGACACTGTCAAGC-1 pbmc3k 1939 720 1.7019082 1 1
AGACCTGAAGTAGA-1 pbmc3k 2590 1011 2.0463320 1 1
AGACCTGACCAACA-1 pbmc3k 2995 1007 2.9716194 2 2
AGACCTGAGGAAGC-1 pbmc3k 2875 1075 1.8782609 4 4
AGACGTACAGAGGC-1 pbmc3k 2190 805 1.2785388 2 2
AGACGTACCCCTAC-1 pbmc3k 1516 671 3.1002639 3 3
AGACGTACCTCTTA-1 pbmc3k 2884 948 4.1955617 1 1
AGACGTACTCGTGA-1 pbmc3k 2242 862 4.1480821 5 5
AGACTGACCATCAG-1 pbmc3k 1894 765 3.4318902 4 4
AGACTGACCCTTTA-1 pbmc3k 2910 962 2.7147766 0 0
AGACTTCTCATGCA-1 pbmc3k 1302 507 2.8417819 3 3
AGAGATGACAGTCA-1 pbmc3k 1339 569 3.2113518 3 3
AGAGATGACTGAAC-1 pbmc3k 1972 676 2.3833671 0 0
AGAGATGAGGTTTG-1 pbmc3k 2058 766 2.8668610 1 1
AGAGATGATCTCGC-1 pbmc3k 3369 1035 3.6509350 3 3
AGAGATGATTGTGG-1 pbmc3k 2338 884 1.5825492 0 0
AGAGCGGAGGCAAG-1 pbmc3k 5226 1422 1.3585917 1 1
AGAGTCTGGTCGTA-1 pbmc3k 4469 1496 3.5578429 5 5
AGAGTGCTCAGCTA-1 pbmc3k 1566 587 1.9157088 0 0
AGAGTGCTCGAATC-1 pbmc3k 2529 876 1.9770660 0 0
AGAGTGCTGTCATG-1 pbmc3k 3579 923 1.5088013 2 2
AGAGTGCTGTCCTC-1 pbmc3k 1943 782 2.1101390 4 4
AGAGTGCTGTGTTG-1 pbmc3k 1634 695 3.2435741 3 3
AGATATACCCGTAA-1 pbmc3k 2258 1055 1.5057573 6 6
AGATATACGATGAA-1 pbmc3k 2385 782 4.8218029 2 2
AGATATACTGTTCT-1 pbmc3k 2358 867 1.1874470 3 3
AGATATTGCCTACC-1 pbmc3k 2393 917 1.0029252 4 4
AGATATTGGCCAAT-1 pbmc3k 4073 1248 1.4485637 1 1
AGATCGTGTCTGGA-1 pbmc3k 2985 1020 1.1725293 1 1
AGATCGTGTTTGTC-1 pbmc3k 1839 722 1.1963023 3 3
AGATCTCTATCACG-1 pbmc3k 1498 642 2.7369826 3 3
AGATTAACGTTCTT-1 pbmc3k 3874 1215 2.3231802 5 5
AGATTCCTATCGTG-1 pbmc3k 2499 929 0.8403361 0 0
AGATTCCTCACTTT-1 pbmc3k 2199 791 2.7285130 1 1
AGATTCCTGACGAG-1 pbmc3k 2735 993 2.5959781 1 1
AGATTCCTGTTCAG-1 pbmc3k 2315 1022 3.1965443 6 6
AGCAAAGATATGCG-1 pbmc3k 2432 944 2.2615132 0 0
AGCACAACAGTCTG-1 pbmc3k 1624 599 2.0935961 0 0
AGCACTGAGGGAGT-1 pbmc3k 2620 880 0.9160305 2 2
AGCACTGATATGCG-1 pbmc3k 2297 975 0.9142360 2 2
AGCACTGATGCTTT-1 pbmc3k 5149 1605 2.1946009 7 7
AGCACTGATTGCGA-1 pbmc3k 2156 756 1.8552876 0 0
AGCATCGAAGATCC-1 pbmc3k 1726 772 2.3754345 2 2
AGCATCGAAGGGTG-1 pbmc3k 2171 681 1.6582220 3 3
AGCATCGAGCTTCC-1 pbmc3k 2581 992 3.9519566 1 1
AGCATCGAGTGAGG-1 pbmc3k 2979 979 1.7455522 2 2
AGCATCGATAACCG-1 pbmc3k 2780 1045 1.3669065 1 1
AGCATGACGATGAA-1 pbmc3k 1869 705 2.0866774 4 4
AGCCAATGGGGAGT-1 pbmc3k 1876 808 1.5991471 5 5
AGCCAATGTATCTC-1 pbmc3k 2616 854 1.7966361 0 0
AGCCACCTGGATCT-1 pbmc3k 1983 819 2.2188603 4 4
AGCCGGTGCCAATG-1 pbmc3k 3728 1146 1.0729614 2 2
AGCCGGTGTGTTTC-1 pbmc3k 2195 741 1.4578588 0 0
AGCCGTCTCAATCG-1 pbmc3k 2919 914 4.2822885 0 0
AGCCGTCTGAGAGC-1 pbmc3k 3003 967 2.5974026 2 2
AGCCTCACGTTCGA-1 pbmc3k 2157 704 1.6689847 0 0
AGCCTCACTGTCAG-1 pbmc3k 4811 1393 1.4134276 5 5
AGCCTCTGCAGTTG-1 pbmc3k 1473 581 2.5118805 3 3
AGCCTCTGCCAATG-1 pbmc3k 1677 807 1.6696482 6 6
AGCGAACTGGATCT-1 pbmc3k 3137 919 2.6777176 0 0
AGCGAACTTACTGG-1 pbmc3k 2115 803 1.4184397 2 2
AGCGATACGGAGCA-1 pbmc3k 1588 540 1.9521411 0 0
AGCGATTGAGATCC-1 pbmc3k 1918 887 2.1897810 6 6
AGCGCCGAATCTCT-1 pbmc3k 1279 519 1.2509773 0 0
AGCGCCGACAGAGG-1 pbmc3k 2676 1090 1.6442451 2 2
AGCGCTCTACCTTT-1 pbmc3k 1767 762 1.4714205 0 0
AGCGGCACCGGGAA-1 pbmc3k 2103 698 1.6167380 0 0
AGCGGCTGATGTGC-1 pbmc3k 2266 749 1.6328332 0 0
AGCGGGCTTGCCAA-1 pbmc3k 2243 770 1.5158270 0 0
AGCGTAACATGCTG-1 pbmc3k 2423 898 1.9397441 2 2
AGCGTAACTGAGAA-1 pbmc3k 3077 1097 1.9824504 1 1
AGCTCGCTACTGGT-1 pbmc3k 2528 757 2.0965190 0 0
AGCTCGCTCTGCTC-1 pbmc3k 2673 881 1.4590348 0 0
AGCTGAACCATACG-1 pbmc3k 3215 1121 3.7636081 5 5
AGCTGAACCTCTCG-1 pbmc3k 2319 808 1.8973696 0 0
AGCTGCCTTGGGAG-1 pbmc3k 1037 445 4.4358727 1 1
AGCTGCCTTTCATC-1 pbmc3k 5212 1703 2.9547199 2 2
AGCTGCCTTTCTGT-1 pbmc3k 2278 786 2.0632133 2 2
AGCTGTGATCCAAG-1 pbmc3k 1548 638 3.6175711 0 0
AGCTTTACAAGTAG-1 pbmc3k 2258 816 1.6386182 0 0
AGCTTTACACCAAC-1 pbmc3k 2112 888 3.1250000 4 4
AGCTTTACTCTCAT-1 pbmc3k 2566 763 1.5978176 3 3
AGGAAATGAGGAGC-1 pbmc3k 1879 805 0.6918574 2 2
AGGAACCTCTTAGG-1 pbmc3k 3842 1321 2.9411765 5 5
AGGAACCTTGCCTC-1 pbmc3k 2187 941 1.8747142 4 4
AGGAATGATAACGC-1 pbmc3k 1975 807 2.7848101 2 2
AGGAATGATTTGTC-1 pbmc3k 2644 977 1.3237519 2 2
AGGAGTCTGGTTTG-1 pbmc3k 2506 886 2.3942538 0 0
AGGAGTCTTGTCAG-1 pbmc3k 1977 725 1.6186141 0 0
AGGATAGACATTTC-1 pbmc3k 4142 1187 1.1105746 2 2
AGGATAGAGGATTC-1 pbmc3k 1962 825 1.0703364 4 4
AGGATGCTACTAGC-1 pbmc3k 1803 659 1.1647255 3 3
AGGATGCTTTAGGC-1 pbmc3k 1401 713 2.2127052 6 6
AGGCAACTGAAGGC-1 pbmc3k 2696 814 2.7818991 0 0
AGGCAGGAGTACCA-1 pbmc3k 2150 820 3.7674419 4 4
AGGCCTCTAGTCGT-1 pbmc3k 2264 806 0.7950530 0 0
AGGCCTCTCGGAGA-1 pbmc3k 2388 864 2.0519263 2 2
AGGCCTCTCGTAAC-1 pbmc3k 1204 463 0.6644518 0 0
AGGGACGACGTTGA-1 pbmc3k 1856 663 2.1551724 0 0
AGGGACGAGTCAAC-1 pbmc3k 1955 929 1.5345269 6 6
AGGGACGAGTTGTG-1 pbmc3k 2746 860 1.3109978 0 0
AGGGACGATAGAGA-1 pbmc3k 1719 671 3.8976149 4 4
AGGGACGATGCATG-1 pbmc3k 1783 803 1.0656197 4 4
AGGGAGTGAGCCTA-1 pbmc3k 997 464 0.8024072 0 0
AGGGCCACCATACG-1 pbmc3k 2047 788 1.6121153 0 0
AGGGCGCTAACCAC-1 pbmc3k 3558 1068 2.4451939 1 1
AGGGCGCTATGGTC-1 pbmc3k 1609 781 4.4748291 2 2
AGGGTGGACAGTCA-1 pbmc3k 2229 843 2.4674742 3 3
AGGGTGGACTCAAG-1 pbmc3k 2072 779 2.1235521 1 1
AGGGTGGAGTTGCA-1 pbmc3k 2252 872 1.8206039 4 4
AGGGTTTGTTCATC-1 pbmc3k 1794 706 2.1739130 1 1
AGGTCATGAGTGTC-1 pbmc3k 925 492 3.2432432 3 3
AGGTCATGCTTATC-1 pbmc3k 1586 667 2.0176545 4 4
AGGTCTGATTCTCA-1 pbmc3k 2730 981 2.0879121 5 5
AGGTGGGAAGAATG-1 pbmc3k 2033 749 1.4756517 2 2
AGGTGGGAAGTTCG-1 pbmc3k 2473 788 1.3748484 0 0
AGGTGTTGGTTACG-1 pbmc3k 2090 780 1.2918660 3 3
AGGTTCGAACCTCC-1 pbmc3k 1346 561 1.8573551 1 1
AGGTTCGAACGTAC-1 pbmc3k 1592 655 1.4447236 1 1
AGGTTCGAGGGTGA-1 pbmc3k 1154 431 2.3396880 0 0
AGTAAGGAGTTTGG-1 pbmc3k 1928 801 1.6597510 2 2
AGTAAGGATTCTTG-1 pbmc3k 2893 944 0.9678534 2 2
AGTAATACATCACG-1 pbmc3k 1831 769 2.3484435 6 6
AGTAATACCGAACT-1 pbmc3k 1236 503 2.5080906 3 3
AGTAATTGTCCCAC-1 pbmc3k 1892 766 1.4799154 2 2
AGTACGTGAGGGTG-1 pbmc3k 2386 776 1.6345348 0 0
AGTACGTGCTGCAA-1 pbmc3k 2998 967 1.2675117 0 0
AGTACGTGCTTGGA-1 pbmc3k 3503 986 1.2275193 2 2
AGTACTCTACGTGT-1 pbmc3k 1603 653 1.8714910 3 3
AGTACTCTCAACCA-1 pbmc3k 1766 762 4.8697622 4 4
AGTACTCTCGGTAT-1 pbmc3k 2063 882 1.5511391 2 2
AGTAGGCTTGCCTC-1 pbmc3k 3555 1230 2.7848101 5 5
AGTATAACTTGTCT-1 pbmc3k 4454 1498 3.2105972 5 5
AGTATCCTAGAACA-1 pbmc3k 1824 625 0.8771930 0 0
AGTCACGATGAGCT-1 pbmc3k 3717 1378 1.4527845 6 6
AGTCAGACGAATAG-1 pbmc3k 3303 1168 1.6651529 2 2
AGTCAGACGCTTAG-1 pbmc3k 1011 491 3.1651830 0 0
AGTCAGACTAGAGA-1 pbmc3k 1992 831 1.9076305 4 4
AGTCAGACTGCACA-1 pbmc3k 2820 924 1.4893617 2 2
AGTCCAGATATCTC-1 pbmc3k 1746 581 1.9473081 0 0
AGTCCAGATTTCAC-1 pbmc3k 2095 842 1.5751790 2 2
AGTCGAACCAACCA-1 pbmc3k 1546 595 2.3285899 0 0
AGTCGCCTCCGTAA-1 pbmc3k 2231 811 0.7619901 2 2
AGTCTACTAGGGTG-1 pbmc3k 1190 540 0.9243697 1 1
AGTCTACTTGCATG-1 pbmc3k 5201 1569 3.3262834 5 5
AGTCTTACACCACA-1 pbmc3k 2421 754 1.8587361 0 0
AGTCTTACTTCGCC-1 pbmc3k 2126 851 3.5747883 4 4
AGTCTTACTTCGGA-1 pbmc3k 663 273 0.7541478 8 8
AGTGACTGCAACTG-1 pbmc3k 4696 1412 3.0877342 5 5
AGTGTTCTAACCTG-1 pbmc3k 2858 872 0.9447166 2 2
AGTGTTCTATAAGG-1 pbmc3k 1308 558 2.2935780 0 0
AGTGTTCTCACTTT-1 pbmc3k 2772 907 1.2265512 1 1
AGTTAAACCACTTT-1 pbmc3k 3021 948 2.7143330 2 2
AGTTATGAACAGTC-1 pbmc3k 3623 1174 1.6284847 1 1
AGTTATGACTGAGT-1 pbmc3k 2087 946 0.5749880 2 2
AGTTATGAGTTCAG-1 pbmc3k 2862 904 2.9350105 2 2
AGTTCTACCAGCTA-1 pbmc3k 1632 737 2.4509804 1 1
AGTTCTTGAAGCCT-1 pbmc3k 1278 603 2.8169014 4 4
AGTTGTCTACTACG-1 pbmc3k 2877 841 1.9464720 0 0
AGTTTAGATGGTCA-1 pbmc3k 1395 606 2.4372760 0 0
AGTTTCACGGTCTA-1 pbmc3k 2519 1078 1.1512505 4 4
AGTTTGCTACAGTC-1 pbmc3k 2090 666 1.7703349 0 0
AGTTTGCTACTGGT-1 pbmc3k 1940 783 3.3505155 4 4
AGTTTGCTCCAAGT-1 pbmc3k 2050 726 0.5365854 0 0
ATAAACACAGTGCT-1 pbmc3k 2500 978 1.8400000 3 3
ATAAACACCACCAA-1 pbmc3k 2917 940 3.6681522 0 0
ATAACAACATGCTG-1 pbmc3k 2231 994 3.0479606 1 1
ATAACAACGTCTAG-1 pbmc3k 1670 653 2.8742515 0 0
ATAACAACTTTGTC-1 pbmc3k 3129 1010 2.3330137 0 0
ATAACATGTACTCT-1 pbmc3k 2775 986 1.4414414 2 2
ATAACCCTGTTGGT-1 pbmc3k 1638 591 3.0525031 0 0
ATAACCCTTGGTAC-1 pbmc3k 2947 1039 0.6107906 2 2
ATAAGTACGAATGA-1 pbmc3k 1560 623 1.6666667 3 3
ATAAGTTGGTACGT-1 pbmc3k 1354 581 2.4372230 5 5
ATAAGTTGTCTAGG-1 pbmc3k 2337 813 3.4659820 2 2
ATAATCGAGCTGAT-1 pbmc3k 1385 569 1.9494585 3 3
ATAATCGATGGTTG-1 pbmc3k 2348 775 0.5536627 0 0
ATAATGACCTACTT-1 pbmc3k 2212 819 1.2206148 2 2
ATAATGACTCGTGA-1 pbmc3k 1842 714 2.5515744 1 1
ATACAATGTTAGGC-1 pbmc3k 2145 735 1.6783217 0 0
ATACCACTCGTACA-1 pbmc3k 2893 953 2.0739717 1 1
ATACCACTCTAAGC-1 pbmc3k 4132 1173 2.4201355 7 7
ATACCACTGCCAAT-1 pbmc3k 1579 742 3.3565548 4 4
ATACCGGAATGCTG-1 pbmc3k 6580 1859 2.2948328 7 7
ATACCGGACATTTC-1 pbmc3k 2213 841 1.8978762 1 1
ATACCGGACTTCGC-1 pbmc3k 2693 943 2.2279985 2 2
ATACCGGAGGTGTT-1 pbmc3k 2129 783 2.1606388 1 1
ATACCGGATCTCGC-1 pbmc3k 1748 638 2.6887872 0 0
ATACCTACGCATCA-1 pbmc3k 2759 922 2.0297209 2 2
ATACCTTGGGGCAA-1 pbmc3k 3676 934 1.3873776 0 0
ATACGGACAGACTC-1 pbmc3k 877 431 4.1049031 1 1
ATACGGACCTACTT-1 pbmc3k 1042 490 2.2072937 1 1
ATACGGACGAGGTG-1 pbmc3k 1215 529 3.1275720 3 3
ATACGGACTATGCG-1 pbmc3k 1472 522 2.1739130 3 3
ATACGGACTCTGGA-1 pbmc3k 1468 618 3.4741144 1 1
ATACGTCTTAACGC-1 pbmc3k 2010 748 4.5273632 1 1
ATACTCTGCTTCGC-1 pbmc3k 4471 1331 2.1695370 1 1
ATACTCTGGTATGC-1 pbmc3k 1618 833 2.1631644 6 6
ATAGATACCATGGT-1 pbmc3k 2179 786 1.8357045 2 2
ATAGATACGACGAG-1 pbmc3k 2122 701 2.6861451 0 0
ATAGATTGGTGTAC-1 pbmc3k 2746 889 2.0029133 3 3
ATAGCCGAACGGAG-1 pbmc3k 2094 692 2.6743075 0 0
ATAGCGTGCAGATC-1 pbmc3k 2444 780 1.0638298 2 2
ATAGCGTGCCCTTG-1 pbmc3k 2997 937 1.1344678 1 1
ATAGCGTGGTATCG-1 pbmc3k 1068 470 1.7790262 1 1
ATAGCGTGTCTCTA-1 pbmc3k 2272 838 1.8485915 2 2
ATAGCTCTCTGATG-1 pbmc3k 1163 529 2.9234738 0 0
ATAGCTCTGAGGTG-1 pbmc3k 1974 728 2.1276596 0 0
ATAGGAGAAACAGA-1 pbmc3k 3553 1083 2.1953279 1 1
ATAGGCTGTCAGAC-1 pbmc3k 2171 800 1.6582220 0 0
ATAGTCCTAGTGTC-1 pbmc3k 2203 884 2.1334544 1 1
ATAGTCCTTGCATG-1 pbmc3k 2055 815 0.7785888 4 4
ATAGTCCTTGTCGA-1 pbmc3k 2658 852 1.1286682 0 0
ATAGTTGACAACTG-1 pbmc3k 2182 744 1.6040330 0 0
ATAGTTGACCCTCA-1 pbmc3k 4272 1317 1.8726592 5 5
ATAGTTGAGACGTT-1 pbmc3k 2027 542 3.0093735 0 0
ATAGTTGATAAGCC-1 pbmc3k 1870 696 1.6577540 0 0
ATATACGAAGCCAT-1 pbmc3k 2505 872 1.6367265 0 0
ATATACGAATTGGC-1 pbmc3k 3930 1150 1.3231552 2 2
ATATAGTGGAATGA-1 pbmc3k 1061 466 2.9217719 1 1
ATATGCCTAGATCC-1 pbmc3k 1910 725 2.0418848 1 1
ATATGCCTGGACAG-1 pbmc3k 3508 1026 2.1664766 2 2
ATATGCCTTCTCTA-1 pbmc3k 1090 479 1.3761468 2 2
ATATGCCTTGGTAC-1 pbmc3k 2020 727 1.7326733 0 0
ATCAAATGAGCCTA-1 pbmc3k 2561 925 3.1237798 1 1
ATCAAATGGGTAAA-1 pbmc3k 4209 1280 2.2808268 5 5
ATCAACCTAAACGA-1 pbmc3k 2638 907 0.8339651 2 2
ATCAACCTGAGGAC-1 pbmc3k 2311 832 2.7260926 0 0
ATCAACCTTCTCTA-1 pbmc3k 2504 1023 2.9952077 4 4
ATCAACCTTTGTCT-1 pbmc3k 2571 837 1.9058732 4 4
ATCACACTTTGTCT-1 pbmc3k 2559 899 2.1101993 2 2
ATCACGGATTTCGT-1 pbmc3k 1788 733 2.1812081 3 3
ATCATCTGACACCA-1 pbmc3k 736 366 0.6793478 8 8
ATCATGCTAGAGTA-1 pbmc3k 848 457 3.4198113 4 4
ATCATGCTGAACCT-1 pbmc3k 3164 1029 2.3388116 3 3
ATCCAGGACGCTAA-1 pbmc3k 3754 1277 1.2519979 5 5
ATCCAGGATGGAAA-1 pbmc3k 2125 753 1.2705882 2 2
ATCCATACTCCTTA-1 pbmc3k 1622 660 2.5277435 1 1
ATCCATACTTCATC-1 pbmc3k 3914 1321 2.3760858 5 5
ATCCCGTGCAGTCA-1 pbmc3k 1995 1002 1.9548872 6 6
ATCCCGTGCATGCA-1 pbmc3k 775 358 2.8387097 3 3
ATCCCGTGGCTGAT-1 pbmc3k 2155 770 1.8097448 3 3
ATCCGCACGCATCA-1 pbmc3k 2341 813 2.0931226 3 3
ATCCTAACGACGGA-1 pbmc3k 1776 634 3.6599099 3 3
ATCCTAACGCTACA-1 pbmc3k 2062 922 1.6973812 4 4
ATCGACGAAACTGC-1 pbmc3k 3305 1181 1.1195159 1 1
ATCGACGAATGACC-1 pbmc3k 2867 901 3.6623648 0 0
ATCGAGTGGACGTT-1 pbmc3k 1972 815 1.6734280 4 4
ATCGCAGAATCTCT-1 pbmc3k 4337 1418 2.5593728 5 5
ATCGCAGAGTGTCA-1 pbmc3k 2108 770 1.4705882 3 3
ATCGCCACTGAGGG-1 pbmc3k 1687 706 2.8452875 4 4
ATCGCCTGGGTCAT-1 pbmc3k 2874 952 2.0180932 0 0
ATCGCCTGTGGCAT-1 pbmc3k 756 348 1.7195767 3 3
ATCGCGCTCAGAGG-1 pbmc3k 1841 715 3.3134166 1 1
ATCGCGCTGGGATG-1 pbmc3k 2731 869 1.4646650 2 2
ATCGCGCTTTTCGT-1 pbmc3k 1779 715 2.5857223 3 3
ATCGGAACCAGTCA-1 pbmc3k 1199 550 0.9174312 0 0
ATCGGTGAGTCAAC-1 pbmc3k 1885 689 1.6976127 0 0
ATCGGTGATTGCAG-1 pbmc3k 2901 947 2.8955533 0 0
ATCGTTTGCCTACC-1 pbmc3k 3402 1155 2.2339800 1 1
ATCGTTTGGGTACT-1 pbmc3k 1764 689 3.2879819 1 1
ATCGTTTGTGCCAA-1 pbmc3k 737 383 1.8995929 1 1
ATCTACACCCGCTT-1 pbmc3k 753 373 1.7264276 1 1
ATCTACACCGGGAA-1 pbmc3k 2450 878 0.7346939 2 2
ATCTCAACAGGAGC-1 pbmc3k 1914 696 2.2466040 3 3
ATCTCAACCTCGAA-1 pbmc3k 609 246 0.0000000 0 0
ATCTCAACCTTGTT-1 pbmc3k 2135 830 2.1077283 1 1
ATCTGGGAAACCAC-1 pbmc3k 2418 1004 2.0678246 1 1
ATCTGGGAAGTGTC-1 pbmc3k 3474 1178 1.7271157 1 1
ATCTGGGATTCCGC-1 pbmc3k 2770 973 2.6353791 4 4
ATCTGTTGAACGGG-1 pbmc3k 2309 855 1.6024253 2 2
ATCTGTTGACCTCC-1 pbmc3k 2711 976 1.4016968 0 0
ATCTGTTGCCTTCG-1 pbmc3k 5197 1550 1.8087358 5 5
ATCTGTTGGTTGCA-1 pbmc3k 2910 964 1.3058419 2 2
ATCTTGACACCAAC-1 pbmc3k 2702 907 1.0362694 2 2
ATCTTGACCTCCCA-1 pbmc3k 1399 565 3.1451036 1 1
ATCTTTCTGCATCA-1 pbmc3k 875 415 1.4857143 0 0
ATCTTTCTGTTTCT-1 pbmc3k 2364 905 1.0152284 4 4
ATCTTTCTTGTCCC-1 pbmc3k 1911 923 3.5060178 6 6
ATGAAACTCTGTGA-1 pbmc3k 3205 966 1.1232449 2 2
ATGAAACTGAGGCA-1 pbmc3k 1972 657 2.2312373 0 0
ATGAAGGAACAGCT-1 pbmc3k 1474 724 1.8317503 4 4
ATGAAGGACCTGTC-1 pbmc3k 2047 743 3.7616023 3 3
ATGAAGGACCTTAT-1 pbmc3k 859 381 2.4447031 3 3
ATGAAGGACTAGTG-1 pbmc3k 712 384 1.9662921 3 3
ATGAAGGACTTGCC-1 pbmc3k 1799 904 1.5008338 6 6
ATGACGTGACGACT-1 pbmc3k 1881 645 1.1695906 3 3
ATGACGTGATCGGT-1 pbmc3k 3623 1167 3.6157880 5 5
ATGAGAGAAAGTGA-1 pbmc3k 2879 935 0.7294199 2 2
ATGAGAGAACGCAT-1 pbmc3k 2348 722 1.1073254 0 0
ATGAGAGAAGTAGA-1 pbmc3k 1575 618 3.3650794 3 3
ATGAGCACACAGCT-1 pbmc3k 3383 1034 1.9213716 0 0
ATGAGCACATCTTC-1 pbmc3k 2598 852 1.1932256 0 0
ATGATAACTTCACT-1 pbmc3k 1522 807 1.0512484 6 6
ATGATATGAAACAG-1 pbmc3k 4921 1399 1.5850437 2 2
ATGATATGACTGGT-1 pbmc3k 2341 760 2.0931226 0 0
ATGATATGAGCACT-1 pbmc3k 2188 740 1.2340037 0 0
ATGATATGGTCATG-1 pbmc3k 1768 687 1.6968326 0 0
ATGATATGGTGCTA-1 pbmc3k 1974 929 2.7355623 6 6
ATGATATGTTGTCT-1 pbmc3k 2991 1095 2.7081244 1 1
ATGCACGAATGTCG-1 pbmc3k 1380 600 3.1884058 3 3
ATGCACGACTGTAG-1 pbmc3k 1477 650 1.6926202 0 0
ATGCACGAGAACCT-1 pbmc3k 2622 893 1.3348589 3 3
ATGCACGAGTTCGA-1 pbmc3k 1919 681 1.9801980 0 0
ATGCACGATTGGTG-1 pbmc3k 4854 1551 1.9365472 1 1
ATGCAGTGTTACCT-1 pbmc3k 1395 603 2.0788530 3 3
ATGCAGTGTTCTAC-1 pbmc3k 2330 820 3.1330472 2 2
ATGCCAGAACGACT-1 pbmc3k 2167 803 1.9843101 0 0
ATGCCAGACAGTCA-1 pbmc3k 1323 646 2.8722600 1 1
ATGCCGCTTGAACC-1 pbmc3k 2460 914 2.7235772 1 1
ATGCGATGCTATGG-1 pbmc3k 2996 940 3.3377837 1 1
ATGCGATGCTGAGT-1 pbmc3k 2539 846 1.5360378 0 0
ATGCGATGGTTACG-1 pbmc3k 2229 794 1.3458950 3 3
ATGCGCCTTCATTC-1 pbmc3k 2542 769 2.6357199 0 0
ATGCTTTGCGAATC-1 pbmc3k 2481 948 2.9423620 2 2
ATGCTTTGGGCGAA-1 pbmc3k 1082 515 2.3105360 4 4
ATGCTTTGTAGTCG-1 pbmc3k 1680 662 1.6071429 3 3
ATGGACACATCGGT-1 pbmc3k 3092 999 1.5200517 2 2
ATGGACACGCATCA-1 pbmc3k 1025 637 2.7317073 6 6
ATGGGTACAACCTG-1 pbmc3k 1346 617 2.8231798 3 3
ATGGGTACATCGGT-1 pbmc3k 1873 907 1.6550988 6 6
ATGGGTACTATTCC-1 pbmc3k 2243 960 0.8916630 2 2
ATGGGTACTGGGAG-1 pbmc3k 2458 812 1.5459723 2 2
ATGTAAACACCTCC-1 pbmc3k 2155 791 1.7633411 0 0
ATGTAAACCCGCTT-1 pbmc3k 2956 1040 1.3531800 2 2
ATGTAAACGGGATG-1 pbmc3k 3960 1342 3.5353535 7 7
ATGTAAACTCTCCG-1 pbmc3k 4766 1323 2.6227444 2 2
ATGTAAACTTCACT-1 pbmc3k 2704 978 2.4408284 1 1
ATGTACCTCAGTCA-1 pbmc3k 2075 672 2.4578313 0 0
ATGTACCTTAGTCG-1 pbmc3k 2213 936 0.4066878 2 2
ATGTACCTTTATCC-1 pbmc3k 1535 630 2.6058632 1 1
ATGTACCTTTCACT-1 pbmc3k 2520 1023 1.6269841 4 4
ATGTCACTAATGCC-1 pbmc3k 3557 1134 1.9679505 1 1
ATGTCACTCTGCTC-1 pbmc3k 1015 498 3.4482759 0 0
ATGTCGGAGGTGAG-1 pbmc3k 2364 948 1.8612521 2 2
ATGTTCACAGTCTG-1 pbmc3k 1917 800 1.9822640 1 1
ATGTTCACCGTAGT-1 pbmc3k 2456 847 2.8501629 2 2
ATGTTGCTTTCAGG-1 pbmc3k 2014 739 1.4399206 2 2
ATTAACGATGAGAA-1 pbmc3k 1291 604 4.1053447 6 6
ATTAACGATGCAAC-1 pbmc3k 2025 863 1.2345679 4 4
ATTAAGACTGCAGT-1 pbmc3k 3364 1074 1.7538644 2 2
ATTACCTGCCTTAT-1 pbmc3k 3534 1104 1.5563101 1 1
ATTACCTGGAGGAC-1 pbmc3k 3447 1109 0.9863650 5 5
ATTAGATGTTTCAC-1 pbmc3k 2060 623 1.4563107 0 0
ATTATGGAATCTCT-1 pbmc3k 2732 1006 0.9150805 2 2
ATTCAAGAACGGGA-1 pbmc3k 3044 907 2.1681997 0 0
ATTCAAGACCTTTA-1 pbmc3k 1973 814 2.1287380 2 2
ATTCAGCTCATTGG-1 pbmc3k 916 345 2.9475983 8 8
ATTCCAACCATTGG-1 pbmc3k 2894 1061 3.1789910 1 1
ATTCCAACTTAGGC-1 pbmc3k 1895 800 2.3218997 6 6
ATTCGACTCACTAG-1 pbmc3k 1771 749 1.4680971 4 4
ATTCGACTGAATAG-1 pbmc3k 2063 778 1.8419777 1 1
ATTCGACTTTTGTC-1 pbmc3k 2614 1024 2.4100995 5 5
ATTCGGGAAAGGCG-1 pbmc3k 2999 1032 2.0006669 1 1
ATTCGGGATTAGGC-1 pbmc3k 3250 1038 0.7076923 2 2
ATTCTTCTGATACC-1 pbmc3k 1721 653 2.0337013 4 4
ATTGAATGGACGGA-1 pbmc3k 1529 630 2.6160889 0 0
ATTGATGAAGGTTC-1 pbmc3k 2344 755 2.4744027 0 0
ATTGATGACTGAGT-1 pbmc3k 2789 850 1.3983507 0 0
ATTGATGAGCGAAG-1 pbmc3k 2290 850 1.5720524 2 2
ATTGATGATCTATC-1 pbmc3k 2246 796 2.2707035 0 0
ATTGCACTGACGGA-1 pbmc3k 3061 860 2.1561581 0 0
ATTGCACTGAGAGC-1 pbmc3k 2965 1078 1.7200675 5 5
ATTGCACTGGAGCA-1 pbmc3k 1621 690 2.5909932 1 1
ATTGCACTTAGCCA-1 pbmc3k 2391 862 0.8782936 2 2
ATTGCACTTGCTTT-1 pbmc3k 4888 1519 2.2094926 5 5
ATTGCTTGTTACTC-1 pbmc3k 2437 836 1.8875667 3 3
ATTGGTCTGACTAC-1 pbmc3k 2507 966 2.2736338 3 3
ATTGGTCTTGTCTT-1 pbmc3k 752 389 1.3297872 3 3
ATTGTAGATTCCCG-1 pbmc3k 5496 1647 2.3107715 7 7
ATTGTAGATTGCAG-1 pbmc3k 2589 808 2.7809965 0 0
ATTGTCTGCGTACA-1 pbmc3k 2317 885 1.8990073 2 2
ATTTAGGAACCATG-1 pbmc3k 5677 1662 2.8536199 5 5
ATTTAGGACAGAGG-1 pbmc3k 2433 918 1.0686395 2 2
ATTTCCGAGATGAA-1 pbmc3k 2447 853 4.5361667 2 2
ATTTCCGAGTGCTA-1 pbmc3k 1907 806 2.9889879 2 2
ATTTCGTGTATGGC-1 pbmc3k 2244 859 2.0944742 4 4
ATTTCTCTACTTTC-1 pbmc3k 4148 1273 2.8688525 5 5
ATTTCTCTAGCAAA-1 pbmc3k 2439 985 1.3530135 2 2
ATTTCTCTCACTTT-1 pbmc3k 3223 1079 2.0477816 5 5
ATTTCTCTTCCCAC-1 pbmc3k 1973 677 1.3684744 0 0
ATTTGCACAAGATG-1 pbmc3k 1525 626 4.1967213 1 1
CAAAGCACAGCTCA-1 pbmc3k 2392 830 1.3377926 0 0
CAAAGCACCGTAAC-1 pbmc3k 2344 826 1.7918089 3 3
CAAAGCACGGTAAA-1 pbmc3k 2136 1007 1.4044944 4 4
CAAAGCTGAAAGTG-1 pbmc3k 1234 547 3.1604538 0 0
CAAAGCTGTTGCTT-1 pbmc3k 2484 891 2.3752013 1 1
CAAATATGTGACAC-1 pbmc3k 2407 722 2.3680931 0 0
CAAATTGAGGGCAA-1 pbmc3k 1988 656 1.9617706 0 0
CAAATTGATGGAGG-1 pbmc3k 845 414 3.9053254 1 1
CAACCAGAAAAGTG-1 pbmc3k 2234 770 2.5067144 0 0
CAACCAGAAGTGCT-1 pbmc3k 4328 1262 2.5646950 2 2
CAACCAGAGTTCAG-1 pbmc3k 3776 1218 2.8866525 1 1
CAACCAGATAGAAG-1 pbmc3k 738 364 1.8970190 1 1
CAACCGCTGTTCAG-1 pbmc3k 1990 660 3.5678392 0 0
CAACCGCTTTGAGC-1 pbmc3k 2299 805 2.3923445 2 2
CAACGATGCGCAAT-1 pbmc3k 4517 1407 2.0588886 1 1
CAACGTGACTCCAC-1 pbmc3k 2996 1091 2.9372497 1 1
CAACGTGAGCCATA-1 pbmc3k 1667 779 1.2597481 4 4
CAACGTGATCAAGC-1 pbmc3k 2365 898 2.1987315 1 1
CAAGAAGACCACAA-1 pbmc3k 2019 828 3.6156513 4 4
CAAGAAGACGTCTC-1 pbmc3k 3487 1241 2.3802696 1 1
CAAGAAGATTCTAC-1 pbmc3k 2798 862 1.2866333 0 0
CAAGACTGACCTGA-1 pbmc3k 1669 576 1.9173158 3 3
CAAGACTGAGTAGA-1 pbmc3k 2611 900 1.8000766 3 3
CAAGCTGACCATAG-1 pbmc3k 3771 1272 2.8639618 5 5
CAAGCTGATCTATC-1 pbmc3k 1959 851 0.8677897 4 4
CAAGGACTGTTCAG-1 pbmc3k 3129 1002 2.3649728 0 0
CAAGGACTTCTTTG-1 pbmc3k 1688 719 3.4952607 4 4
CAAGGTTGCTCCAC-1 pbmc3k 1893 760 1.7432647 2 2
CAAGGTTGTCATTC-1 pbmc3k 1864 679 3.2725322 3 3
CAAGGTTGTCTGGA-1 pbmc3k 2040 957 1.9117647 6 6
CAAGTCGAAACAGA-1 pbmc3k 2294 831 4.7515257 3 3
CAAGTCGATAGCGT-1 pbmc3k 1723 723 3.3662217 1 1
CAATAAACGCCATA-1 pbmc3k 829 354 3.4981906 1 1
CAATAATGAACTGC-1 pbmc3k 2252 760 3.1083481 1 1
CAATATGACATGGT-1 pbmc3k 2766 910 2.7114967 2 2
CAATATGACCTTCG-1 pbmc3k 1878 749 1.8104366 4 4
CAATATGACGTTAG-1 pbmc3k 1690 625 3.2544379 0 0
CAATATGAGGAGCA-1 pbmc3k 3036 869 1.2845850 0 0
CAATCGGAGAAACA-1 pbmc3k 2629 794 1.1411183 0 0
CAATCTACTGACTG-1 pbmc3k 2744 1078 1.9314869 6 6
CAATTCACCCAACA-1 pbmc3k 1845 736 3.0352304 4 4
CAATTCACGATAGA-1 pbmc3k 2478 834 1.5738499 0 0
CAATTCACTTGTGG-1 pbmc3k 1032 402 1.7441860 0 0
CAATTCTGCTTGTT-1 pbmc3k 3340 1200 2.3053892 1 1
CAATTCTGGCGTAT-1 pbmc3k 1684 699 3.7410926 5 5
CACAACGATACGAC-1 pbmc3k 2403 753 2.8714107 0 0
CACAATCTTGTTCT-1 pbmc3k 1546 696 1.9404916 4 4
CACAATCTTTCCAT-1 pbmc3k 3851 1227 2.5447936 2 2
CACACCTGCTTGAG-1 pbmc3k 1594 637 1.0037641 0 0
CACACCTGTATGGC-1 pbmc3k 1875 666 4.7466667 0 0
CACAGAACCCTTGC-1 pbmc3k 2017 844 3.0242935 3 3
CACAGAACCTGATG-1 pbmc3k 2837 1035 3.8773352 2 2
CACAGATGGGATTC-1 pbmc3k 3229 1026 1.9820378 2 2
CACAGATGGTTTCT-1 pbmc3k 2088 829 1.5325670 1 1
CACAGCCTGATACC-1 pbmc3k 2829 997 2.5450689 4 4
CACAGCCTTGCCAA-1 pbmc3k 2292 940 2.9232112 5 5
CACAGCCTTGTAGC-1 pbmc3k 2553 889 1.4492754 0 0
CACAGTGATGAAGA-1 pbmc3k 3015 1006 2.7529022 1 1
CACATACTACAGCT-1 pbmc3k 1852 710 1.5658747 4 4
CACATGGAACACGT-1 pbmc3k 2961 989 1.2495778 0 0
CACATGGAAGTCGT-1 pbmc3k 2450 722 2.4489796 0 0
CACCACTGCCAACA-1 pbmc3k 2259 745 2.0805666 0 0
CACCACTGGCGAAG-1 pbmc3k 2217 917 1.4433920 4 4
CACCCATGTTCTGT-1 pbmc3k 561 333 4.4563280 0 0
CACCGGGAATCGAC-1 pbmc3k 2879 1042 1.1114970 2 2
CACCGGGACGAGAG-1 pbmc3k 2587 894 2.0487051 4 4
CACCGGGACGTGTA-1 pbmc3k 3096 873 1.8087855 0 0
CACCGGGACTTCTA-1 pbmc3k 4295 1610 4.0977881 2 2
CACCGGGACTTGCC-1 pbmc3k 1792 724 4.9665179 4 4
CACCGGGATTCGGA-1 pbmc3k 2328 928 3.6082474 4 4
CACCGTACTAAGGA-1 pbmc3k 1656 600 2.4758454 3 3
CACCGTACTAGCGT-1 pbmc3k 2714 861 2.1002211 0 0
CACCTGACACCCAA-1 pbmc3k 2229 908 1.8393899 4 4
CACCTGACCAGAAA-1 pbmc3k 1694 657 2.1841795 2 2
CACCTGACCTCAAG-1 pbmc3k 1929 775 2.1254536 4 4
CACCTGACGAAAGT-1 pbmc3k 1806 723 1.3289037 3 3
CACCTGACTCGTAG-1 pbmc3k 2118 753 2.6440038 0 0
CACGAAACTTCCGC-1 pbmc3k 2110 740 1.7061611 3 3
CACGACCTCGATAC-1 pbmc3k 1766 690 2.1517554 3 3
CACGCTACAGAAGT-1 pbmc3k 683 378 3.2210835 0 0
CACGCTACTGTTCT-1 pbmc3k 1447 607 2.3496890 3 3
CACGGGACAGAGTA-1 pbmc3k 2966 924 2.2589346 0 0
CACGGGACATAAGG-1 pbmc3k 2340 777 1.5811966 0 0
CACGGGACGTAGGG-1 pbmc3k 2651 967 0.6789891 2 2
CACGGGTGCTTCGC-1 pbmc3k 1789 826 1.2297373 4 4
CACGGGTGGAGGAC-1 pbmc3k 1595 709 2.4451411 4 4
CACGGGTGTGTTTC-1 pbmc3k 897 385 1.0033445 0 0
CACTAACTCCTAAG-1 pbmc3k 1604 689 1.8079800 3 3
CACTAACTGAAAGT-1 pbmc3k 2278 767 1.1413521 0 0
CACTAGGATGATGC-1 pbmc3k 1219 554 2.0508614 3 3
CACTATACCCCGTT-1 pbmc3k 2567 889 1.4413713 3 3
CACTATACGTTTGG-1 pbmc3k 1839 760 2.7732463 4 4
CACTGAGACAGTCA-1 pbmc3k 726 366 3.8567493 1 1
CACTGCACTTCATC-1 pbmc3k 1915 729 2.4543081 2 2
CACTGCTGAGACTC-1 pbmc3k 3138 1102 2.3263225 5 5
CACTGCTGGAAAGT-1 pbmc3k 3107 943 1.8667525 2 2
CACTTAACCGAATC-1 pbmc3k 2252 831 4.8401421 2 2
CACTTAACCGTACA-1 pbmc3k 1521 674 2.6955950 3 3
CACTTTGACTCTAT-1 pbmc3k 1551 651 2.1921341 4 4
CACTTTGAGCTGTA-1 pbmc3k 802 418 1.4962594 0 0
CAGAAGCTCTCAAG-1 pbmc3k 2252 723 1.7761989 0 0
CAGACATGAACGGG-1 pbmc3k 2327 745 1.2892136 0 0
CAGACATGTCGACA-1 pbmc3k 2517 870 1.9070322 2 2
CAGACCCTAAGGTA-1 pbmc3k 4164 1334 2.9779059 5 5
CAGACCCTAATGCC-1 pbmc3k 2291 862 4.8886949 0 0
CAGACCCTAGGAGC-1 pbmc3k 2716 934 2.1723122 2 2
CAGACTGAGTATGC-1 pbmc3k 2711 843 2.0287717 0 0
CAGATCGAATGTCG-1 pbmc3k 2191 720 4.3815609 3 3
CAGATCGACCTGAA-1 pbmc3k 920 431 3.4782609 4 4
CAGATCGATATGGC-1 pbmc3k 2546 782 0.9819324 2 2
CAGATGACATTCTC-1 pbmc3k 2045 1004 2.6405868 6 6
CAGCAATGCCTTCG-1 pbmc3k 2563 833 1.4826375 0 0
CAGCAATGGAGGGT-1 pbmc3k 1696 838 1.8278302 6 6
CAGCAATGGTGCTA-1 pbmc3k 2288 838 1.5734266 0 0
CAGCAATGTCTACT-1 pbmc3k 2372 817 2.6559865 1 1
CAGCAATGTGACCA-1 pbmc3k 1910 698 1.1518325 2 2
CAGCAATGTGAGGG-1 pbmc3k 1075 486 2.4186047 1 1
CAGCACCTAAGCCT-1 pbmc3k 1806 663 2.3809524 0 0
CAGCACCTAGGCGA-1 pbmc3k 1447 576 2.3496890 0 0
CAGCACCTGTAGGG-1 pbmc3k 3870 1289 2.9198966 1 1
CAGCATGACAACCA-1 pbmc3k 3217 1101 3.1706559 1 1
CAGCATGAGACGTT-1 pbmc3k 2155 824 4.0835267 4 4
CAGCCTACCCAACA-1 pbmc3k 2801 879 0.8925384 2 2
CAGCCTTGCTACCC-1 pbmc3k 1841 737 2.6072787 1 1
CAGCCTTGGGGACA-1 pbmc3k 2658 900 0.9781791 2 2
CAGCGGACACCCTC-1 pbmc3k 1884 685 2.3354565 0 0
CAGCGGACCTTTAC-1 pbmc3k 2125 870 2.5882353 6 6
CAGCGTCTAAAGCA-1 pbmc3k 2928 886 2.5956284 2 2
CAGCGTCTTATCGG-1 pbmc3k 2531 872 3.8719874 2 2
CAGCTAGATGTGAC-1 pbmc3k 1260 557 4.2063492 1 1
CAGCTCTGAGGCGA-1 pbmc3k 1959 766 1.1230219 2 2
CAGCTCTGCAAGCT-1 pbmc3k 1570 710 1.1464968 4 4
CAGCTCTGTCGTAG-1 pbmc3k 3301 1134 2.2720388 1 1
CAGCTCTGTGTGGT-1 pbmc3k 2163 1047 1.8492834 6 6
CAGGAACTAACTGC-1 pbmc3k 2077 805 2.4073182 4 4
CAGGAACTCTCAGA-1 pbmc3k 1752 724 1.5981735 1 1
CAGGCCGAACACCA-1 pbmc3k 2693 972 2.1165986 0 0
CAGGCCGAACACGT-1 pbmc3k 3917 1201 1.0977789 2 2
CAGGCCGAACGACT-1 pbmc3k 1193 574 2.7661358 0 0
CAGGCCGAATCTCT-1 pbmc3k 2443 837 1.2689316 5 5
CAGGCCGACTAGCA-1 pbmc3k 2344 863 2.4317406 3 3
CAGGGCACCATACG-1 pbmc3k 1317 545 2.8094153 3 3
CAGGGCACCCAACA-1 pbmc3k 2240 810 2.2767857 0 0
CAGGGCACTCCCGT-1 pbmc3k 2293 884 1.2211077 2 2
CAGGTAACAGACTC-1 pbmc3k 2471 885 2.1044112 1 1
CAGGTATGAGTCGT-1 pbmc3k 2131 787 1.3608634 0 0
CAGGTATGTGCTTT-1 pbmc3k 2709 1025 1.8456995 2 2
CAGGTTGAGGATCT-1 pbmc3k 8011 1996 2.6962926 3 3
CAGTGATGGACGGA-1 pbmc3k 1529 643 1.2426422 3 3
CAGTGATGGCTAAC-1 pbmc3k 1549 763 3.0342156 4 4
CAGTGATGGGACAG-1 pbmc3k 3338 968 2.6363092 2 2
CAGTGATGTAAGGA-1 pbmc3k 2269 800 1.7188189 3 3
CAGTGATGTACGCA-1 pbmc3k 1013 357 3.1589339 3 3
CAGTGTGATGTCAG-1 pbmc3k 2049 623 1.4641288 0 0
CAGTTACTAAGGTA-1 pbmc3k 2855 992 2.8021016 3 3
CAGTTACTGATAGA-1 pbmc3k 2189 797 1.5532206 2 2
CAGTTGGAAAGAGT-1 pbmc3k 3686 1165 1.6006511 1 1
CAGTTGGACATACG-1 pbmc3k 2442 830 1.8018018 0 0
CAGTTTACACACGT-1 pbmc3k 5343 1868 1.3101254 6 6
CAGTTTACCCCAAA-1 pbmc3k 1940 616 2.0103093 0 0
CATAAAACGGAGCA-1 pbmc3k 2351 822 3.3602722 1 1
CATAAATGAACTGC-1 pbmc3k 2063 647 2.7144935 0 0
CATAACCTTCTCCG-1 pbmc3k 3061 999 1.0780791 2 2
CATACTACCTCGAA-1 pbmc3k 1776 616 1.7454955 0 0
CATACTACCTGAAC-1 pbmc3k 2430 733 0.6995885 0 0
CATACTACGTACCA-1 pbmc3k 1618 618 0.9888752 1 1
CATACTTGGGTTAC-1 pbmc3k 7167 1934 2.3580299 5 5
CATAGTCTAATCGC-1 pbmc3k 2908 856 1.8225585 0 0
CATAGTCTCACTTT-1 pbmc3k 2710 942 3.6531365 0 0
CATATAGACTAAGC-1 pbmc3k 2925 1028 1.6410256 7 7
CATATAGATCAGGT-1 pbmc3k 1871 723 2.4585783 3 3
CATCAACTAGAAGT-1 pbmc3k 3377 983 1.8951732 2 2
CATCAACTCCCTCA-1 pbmc3k 3672 993 0.7352941 0 0
CATCAGGACTTCCG-1 pbmc3k 1527 770 2.5540275 6 6
CATCAGGATAGCCA-1 pbmc3k 1500 737 1.8666667 6 6
CATCAGGATCCTAT-1 pbmc3k 5281 1582 2.4237834 5 5
CATCAGGATGCACA-1 pbmc3k 3138 1112 2.9636711 1 1
CATCAGGATTTCGT-1 pbmc3k 1400 614 4.1428571 1 1
CATCATACCGCATA-1 pbmc3k 3300 1144 1.6363636 1 1
CATCATACGGAGCA-1 pbmc3k 1486 615 3.2301480 3 3
CATCATACTCAAGC-1 pbmc3k 2455 847 1.5885947 0 0
CATCGCTGGGATCT-1 pbmc3k 2801 983 1.9635844 2 2
CATCGCTGTGGCAT-1 pbmc3k 1961 676 1.1218766 3 3
CATCGGCTATGCTG-1 pbmc3k 3314 970 1.0863005 2 2
CATCGGCTTTGGCA-1 pbmc3k 2499 789 2.2809124 2 2
CATCTCCTATGTGC-1 pbmc3k 2009 827 2.6381284 3 3
CATCTCCTCGAACT-1 pbmc3k 1857 803 2.4232633 1 1
CATGAGACACGGGA-1 pbmc3k 747 394 3.7483266 3 3
CATGAGACGTTGAC-1 pbmc3k 1758 841 3.4698521 6 6
CATGAGACTCGCCT-1 pbmc3k 1155 493 0.4329004 0 0
CATGCCACGGGTGA-1 pbmc3k 2420 740 3.7603306 0 0
CATGCCACTGCCAA-1 pbmc3k 2396 941 2.5459098 2 2
CATGCGCTAGTCAC-1 pbmc3k 5344 1659 2.3952096 5 5
CATGCGCTCAGATC-1 pbmc3k 2055 741 2.8223844 1 1
CATGCGCTTTGCAG-1 pbmc3k 4790 1515 1.7954071 5 5
CATGGCCTAGGGTG-1 pbmc3k 1346 558 2.5260030 1 1
CATGGCCTGTGCAT-1 pbmc3k 2836 934 2.2566996 0 0
CATGTACTATCGTG-1 pbmc3k 1617 738 1.9789734 4 4
CATGTTACAGTCGT-1 pbmc3k 2186 779 1.9213175 4 4
CATGTTACCTGAGT-1 pbmc3k 2258 818 1.7714792 2 2
CATGTTTGGGGATG-1 pbmc3k 2193 813 2.0975832 1 1
CATTACACACGGAG-1 pbmc3k 1480 578 3.9864865 3 3
CATTACACCAACTG-1 pbmc3k 2357 1019 3.4789987 6 6
CATTACACGGAGTG-1 pbmc3k 3899 1186 1.5132085 0 0
CATTACACTACTCT-1 pbmc3k 2439 823 1.8860189 0 0
CATTAGCTCCACAA-1 pbmc3k 3496 1145 3.6899314 1 1
CATTGACTAGCGGA-1 pbmc3k 1913 755 2.8227914 1 1
CATTGGGACTCGAA-1 pbmc3k 1396 637 3.1518625 0 0
CATTGTACAGCGTT-1 pbmc3k 2230 827 2.2421525 0 0
CATTGTACTCGATG-1 pbmc3k 1856 712 1.6163793 3 3
CATTGTACTTATCC-1 pbmc3k 1616 608 2.9084158 0 0
CATTGTACTTTGCT-1 pbmc3k 4198 1267 2.9776084 5 5
CATTGTTGCTAGTG-1 pbmc3k 2170 680 1.4746544 0 0
CATTTCGACTCTAT-1 pbmc3k 1790 767 2.9050279 4 4
CATTTCGAGATACC-1 pbmc3k 2656 1118 1.0918675 6 6
CATTTGACCACACA-1 pbmc3k 1685 728 2.9080119 4 4
CATTTGACCCTGAA-1 pbmc3k 1728 803 1.3888889 4 4
CATTTGTGACGACT-1 pbmc3k 2454 779 1.7114914 3 3
CATTTGTGCATTGG-1 pbmc3k 2634 914 2.3158694 0 0
CATTTGTGCGGAGA-1 pbmc3k 2442 733 3.7674038 0 0
CATTTGTGGGATCT-1 pbmc3k 2460 834 1.6260163 3 3
CCAAAGTGCTACGA-1 pbmc3k 3438 1253 2.6178010 1 1
CCAAAGTGTGAGAA-1 pbmc3k 1051 472 2.0932445 1 1
CCAACCTGAAGTAG-1 pbmc3k 2139 878 0.7480131 4 4
CCAACCTGACGTAC-1 pbmc3k 2605 848 1.8426104 1 1
CCAACCTGTTCGCC-1 pbmc3k 3211 1020 1.8997197 1 1
CCAAGAACCCAATG-1 pbmc3k 1740 659 1.6666667 2 2
CCAAGAACGTAGCT-1 pbmc3k 3230 1008 1.2693498 0 0
CCAAGAACGTGTCA-1 pbmc3k 2329 744 1.2881065 0 0
CCAAGAACTACTGG-1 pbmc3k 1765 843 2.3229462 4 4
CCAAGAACTCCTAT-1 pbmc3k 838 391 0.7159905 3 3
CCAAGATGTCATTC-1 pbmc3k 1713 670 0.9924110 0 0
CCAAGATGTTTCAC-1 pbmc3k 2093 837 1.9589107 2 2
CCAAGTGAGGAACG-1 pbmc3k 3005 875 2.4625624 0 0
CCAAGTGATCAAGC-1 pbmc3k 2711 938 1.7336776 2 2
CCAATTTGAACGTC-1 pbmc3k 2671 932 1.0482965 1 1
CCACCATGAACGTC-1 pbmc3k 2186 760 1.9213175 0 0
CCACCATGATCGGT-1 pbmc3k 2494 732 1.4835605 0 0
CCACCATGGACGAG-1 pbmc3k 4779 1558 2.7411592 5 5
CCACCATGGGGAGT-1 pbmc3k 2048 750 1.1718750 0 0
CCACCATGTCCTGC-1 pbmc3k 2158 940 1.7145505 4 4
CCACTGACCCGCTT-1 pbmc3k 1953 705 1.8433180 1 1
CCACTGTGGGAAGC-1 pbmc3k 865 404 4.2774566 1 1
CCACTGTGTGTAGC-1 pbmc3k 2305 689 2.6898048 0 0
CCACTTCTCGGGAA-1 pbmc3k 3465 1070 3.6363636 1 1
CCAGAAACCCTGTC-1 pbmc3k 1200 544 1.1666667 3 3
CCAGAAACGAACTC-1 pbmc3k 2588 927 1.3910355 2 2
CCAGAAACGGTCTA-1 pbmc3k 2790 816 1.1827957 0 0
CCAGACCTCTGAGT-1 pbmc3k 794 387 2.3929471 1 1
CCAGACCTTGTGGT-1 pbmc3k 1459 559 2.0562029 0 0
CCAGCACTGCGATT-1 pbmc3k 4088 1320 2.4217221 5 5
CCAGCGGAAAGGCG-1 pbmc3k 1572 640 2.2264631 1 1
CCAGCGGACGACTA-1 pbmc3k 2454 886 2.0782396 1 1
CCAGCGGATGGGAG-1 pbmc3k 3217 1074 1.1190550 2 2
CCAGCTACACAGTC-1 pbmc3k 1758 689 1.5927190 0 0
CCAGCTACCAGCTA-1 pbmc3k 1099 524 1.9108280 3 3
CCAGGTCTACACCA-1 pbmc3k 1367 604 2.9261156 3 3
CCAGGTCTAGCATC-1 pbmc3k 1803 800 1.7193566 1 1
CCAGGTCTATGGTC-1 pbmc3k 2846 865 1.8622628 2 2
CCAGTCACACTGGT-1 pbmc3k 3250 938 3.6923077 1 1
CCAGTCACACTGTG-1 pbmc3k 1761 752 1.3060761 3 3
CCAGTCACGTTGTG-1 pbmc3k 2152 770 1.1617100 0 0
CCAGTGCTAACCAC-1 pbmc3k 3399 1104 1.5004413 2 2
CCAGTGCTCGTAGT-1 pbmc3k 2562 841 2.1467603 2 2
CCATCCGAAAGCAA-1 pbmc3k 2456 785 3.6644951 0 0
CCATCCGAACGACT-1 pbmc3k 929 444 2.5834230 0 0
CCATCCGAAGGTTC-1 pbmc3k 1806 703 1.2735327 3 3
CCATCCGATTCGCC-1 pbmc3k 1314 593 3.1202435 1 1
CCATCGTGAACGGG-1 pbmc3k 2206 874 1.6772439 2 2
CCATCGTGCTAGAC-1 pbmc3k 2559 901 2.1883548 5 5
CCCAACACCTCGCT-1 pbmc3k 2863 924 1.6416346 3 3
CCCAACACGCATCA-1 pbmc3k 2200 797 1.4090909 0 0
CCCAACACTTTGTC-1 pbmc3k 3439 1218 2.0645536 1 1
CCCAACTGCAATCG-1 pbmc3k 853 415 1.6412661 1 1
CCCAGACTGCCTTC-1 pbmc3k 4754 1378 1.9562474 1 1
CCCAGACTGGTTTG-1 pbmc3k 2556 946 1.5258216 3 3
CCCAGACTTTCGCC-1 pbmc3k 4410 1320 2.6077098 5 5
CCCAGTTGCAGTTG-1 pbmc3k 3416 1169 1.8735363 1 1
CCCAGTTGGGTACT-1 pbmc3k 1756 744 2.5626424 4 4
CCCAGTTGTCTATC-1 pbmc3k 1924 726 1.6632017 3 3
CCCGATTGTGTTTC-1 pbmc3k 2271 797 3.2144430 2 2
CCCGGAGAAGGGTG-1 pbmc3k 1019 490 1.6683023 1 1
CCCTACGAATTGGC-1 pbmc3k 1865 767 1.3404826 1 1
CCCTAGTGCAAAGA-1 pbmc3k 2448 943 2.1241830 2 2
CCCTCAGACACTTT-1 pbmc3k 2070 808 1.7391304 3 3
CCCTCAGACGAGAG-1 pbmc3k 2925 925 1.7435897 0 0
CCCTCAGAGGTCAT-1 pbmc3k 2020 660 1.6336634 0 0
CCCTGAACTAAAGG-1 pbmc3k 1530 566 1.9607843 3 3
CCCTGATGCAACCA-1 pbmc3k 2914 843 1.7501716 0 0
CCCTGATGCAAGCT-1 pbmc3k 1846 727 1.8959913 3 3
CCCTTACTAACCAC-1 pbmc3k 3559 1220 2.5288002 1 1
CCCTTACTGCAGTT-1 pbmc3k 973 457 1.8499486 0 0
CCGAAAACCTTGTT-1 pbmc3k 5144 1512 2.5660964 5 5
CCGACACTGGTTTG-1 pbmc3k 3324 1136 1.6847172 2 2
CCGACTACCCAGTA-1 pbmc3k 3182 1170 2.1370207 1 1
CCGACTACCGTGTA-1 pbmc3k 1455 524 1.9243986 0 0
CCGACTACTGAGGG-1 pbmc3k 2370 876 1.7299578 2 2
CCGATAGACCTAAG-1 pbmc3k 1636 523 2.5061125 0 0
CCGATAGAGTTGGT-1 pbmc3k 2958 999 2.0622042 1 1
CCGCGAGACACACA-1 pbmc3k 2108 777 3.4155598 1 1
CCGCGAGAGGTTCA-1 pbmc3k 1965 772 1.9847328 4 4
CCGCTATGGGACGA-1 pbmc3k 1997 816 2.5037556 3 3
CCGCTATGTGCAAC-1 pbmc3k 2523 1015 2.4177566 1 1
CCGCTATGTGCACA-1 pbmc3k 2854 939 2.1723896 1 1
CCGGTACTGTCCTC-1 pbmc3k 2495 855 2.4048096 0 0
CCGTACACAAGCAA-1 pbmc3k 1744 714 1.5481651 0 0
CCGTACACAGCGTT-1 pbmc3k 2007 793 1.2954659 3 3
CCGTACACGTCATG-1 pbmc3k 2076 837 2.9383430 4 4
CCGTACACGTTGGT-1 pbmc3k 3297 1193 2.1838035 1 1
CCGTACACTAACGC-1 pbmc3k 2041 763 3.9196472 4 4
CCTAAACTTTCGTT-1 pbmc3k 2351 832 0.9357720 0 0
CCTAAGGACCCAAA-1 pbmc3k 2053 674 1.7535314 0 0
CCTAAGGACTAGCA-1 pbmc3k 1514 623 3.6988111 1 1
CCTAAGGAGGGCAA-1 pbmc3k 796 420 2.5125628 0 0
CCTAAGGATGATGC-1 pbmc3k 2517 834 1.2713548 0 0
CCTAAGGATGTCAG-1 pbmc3k 2882 874 1.7349063 0 0
CCTACCGACTCTTA-1 pbmc3k 1893 679 1.5847861 0 0
CCTACCGAGGGATG-1 pbmc3k 2343 810 1.6645327 3 3
CCTAGAGAGGTGAG-1 pbmc3k 1387 545 1.4419611 3 3
CCTATAACCAAAGA-1 pbmc3k 2560 785 2.3437500 0 0
CCTATAACGAGACG-1 pbmc3k 1505 586 2.3920266 5 5
CCTATAACTCAGAC-1 pbmc3k 2661 819 1.2401353 0 0
CCTATAACTGCATG-1 pbmc3k 1721 669 2.0918071 5 5
CCTCGAACACTTTC-1 pbmc3k 1644 813 1.9464720 6 6
CCTCGAACCCGTAA-1 pbmc3k 2060 780 2.1844660 5 5
CCTCGAACGTATCG-1 pbmc3k 1617 647 2.5974026 0 0
CCTCGAACTTACTC-1 pbmc3k 3183 923 1.9792648 2 2
CCTCTACTCTTCGC-1 pbmc3k 4464 1322 2.2625448 1 1
CCTCTACTGGCATT-1 pbmc3k 2174 699 1.5179393 0 0
CCTGACTGAAGTAG-1 pbmc3k 2127 791 3.2440056 4 4
CCTGACTGGGGAGT-1 pbmc3k 2542 816 1.3768686 0 0
CCTGACTGTGTCTT-1 pbmc3k 1618 666 3.8936959 3 3
CCTGCAACACGTTG-1 pbmc3k 2093 801 2.2933588 1 1
CCTGGACTCGTGAT-1 pbmc3k 1353 592 1.4042868 2 2
CCTTAATGCCCAAA-1 pbmc3k 1852 739 1.9978402 3 3
CCTTAATGTTCTAC-1 pbmc3k 3832 1241 3.7839248 5 5
CCTTCACTACGACT-1 pbmc3k 2168 859 1.3837638 0 0
CCTTCACTCAGTCA-1 pbmc3k 1418 683 2.8208745 3 3
CCTTCACTGGAGTG-1 pbmc3k 1656 595 2.0531401 1 1
CCTTTAGATTCATC-1 pbmc3k 3505 1191 2.1968616 1 1
CGAACATGCCCTAC-1 pbmc3k 2320 857 2.5000000 1 1
CGAACATGTCAGAC-1 pbmc3k 2210 774 1.4479638 2 2
CGAAGACTGGAACG-1 pbmc3k 2442 945 2.7846028 4 4
CGAAGACTGTTACG-1 pbmc3k 1735 687 2.8242075 2 2
CGAAGGGAAACCTG-1 pbmc3k 2154 903 1.2070566 4 4
CGAAGGGATCCGAA-1 pbmc3k 1834 731 1.9629226 3 3
CGAAGTACCAACTG-1 pbmc3k 1562 618 2.4967990 3 3
CGAATCGAGGAGCA-1 pbmc3k 581 336 3.2702238 1 1
CGAATCGAGGAGGT-1 pbmc3k 2512 922 1.9108280 0 0
CGACAAACCCATAG-1 pbmc3k 2870 801 1.6027875 0 0
CGACAAACCGACAT-1 pbmc3k 2096 810 1.2881679 3 3
CGACCACTAAAGTG-1 pbmc3k 1933 940 2.0175892 6 6
CGACCACTGCCAAT-1 pbmc3k 1849 942 2.9745809 6 6
CGACCGGAAGGTCT-1 pbmc3k 2727 867 2.0168684 0 0
CGACCGGATGGAAA-1 pbmc3k 2696 945 2.3738872 2 2
CGACCTTGCTAGTG-1 pbmc3k 1977 767 0.9104704 2 2
CGACGTCTATCGTG-1 pbmc3k 2042 700 2.3506366 4 4
CGACGTCTCGTGTA-1 pbmc3k 1953 691 2.2529442 2 2
CGACGTCTGAGGCA-1 pbmc3k 1056 422 1.3257576 0 0
CGACTCACGTCGTA-1 pbmc3k 2159 774 2.3158870 0 0
CGACTCACGTTGCA-1 pbmc3k 1093 500 3.1107045 0 0
CGACTCTGTGTGAC-1 pbmc3k 3130 1099 1.5015974 2 2
CGACTGCTTCCTCG-1 pbmc3k 2011 808 1.5912481 1 1
CGAGAACTAAGGCG-1 pbmc3k 1912 727 2.6673640 4 4
CGAGAACTACGTTG-1 pbmc3k 1842 616 2.8230185 0 0
CGAGAACTTGTTCT-1 pbmc3k 2548 925 1.4913658 1 1
CGAGATTGGACACT-1 pbmc3k 1942 834 1.4933059 4 4
CGAGATTGGCCATA-1 pbmc3k 1042 454 2.6871401 0 0
CGAGCCGAACACCA-1 pbmc3k 2385 812 3.1027254 2 2
CGAGCCGAGGCGAA-1 pbmc3k 2445 767 3.2719836 2 2
CGAGCGTGCTCCAC-1 pbmc3k 3298 1053 2.0921771 2 2
CGAGCGTGGATACC-1 pbmc3k 3359 913 1.4289967 2 2
CGAGCGTGTATGCG-1 pbmc3k 1529 627 3.6625245 1 1
CGAGGAGACCTCCA-1 pbmc3k 1530 648 2.2875817 3 3
CGAGGAGATGTCGA-1 pbmc3k 1066 449 3.3771107 0 0
CGAGGCACCTATGG-1 pbmc3k 1973 752 1.8753168 4 4
CGAGGCACTATGCG-1 pbmc3k 4175 1325 2.8982036 1 1
CGAGGCACTCTTCA-1 pbmc3k 1827 776 1.9157088 4 4
CGAGGCTGACGCTA-1 pbmc3k 1371 668 1.9693654 6 6
CGAGGCTGGCAGTT-1 pbmc3k 2937 1014 2.2131427 1 1
CGAGGGCTACGACT-1 pbmc3k 1662 598 2.1058965 3 3
CGAGGGCTCGAATC-1 pbmc3k 904 430 2.8761062 0 0
CGAGTATGTCACCC-1 pbmc3k 2741 963 3.3564393 5 5
CGATACGAACAGTC-1 pbmc3k 3042 1073 1.8080210 1 1
CGATACGACAGGAG-1 pbmc3k 6722 2023 2.2166022 2 2
CGATACGATTCACT-1 pbmc3k 2020 728 1.4356436 3 3
CGATAGACCCGTAA-1 pbmc3k 1221 532 3.6855037 1 1
CGATAGACCGTACA-1 pbmc3k 2090 926 0.7655502 2 2
CGATAGACGTAGGG-1 pbmc3k 1829 724 3.0071077 1 1
CGATAGACTGTTCT-1 pbmc3k 1299 559 2.3094688 0 0
CGATCAGAAGAACA-1 pbmc3k 2198 869 2.1838035 4 4
CGATCAGAGAGGGT-1 pbmc3k 2721 962 2.0213157 0 0
CGATCAGAGGTACT-1 pbmc3k 5139 1338 1.1480833 2 2
CGATCAGATGTGAC-1 pbmc3k 6908 1974 0.9843660 7 7
CGATCCACCGGGAA-1 pbmc3k 3230 1102 2.2291022 4 4
CGATCCACTTCCAT-1 pbmc3k 591 326 2.0304569 3 3
CGCAAATGCTCGAA-1 pbmc3k 1011 427 4.6488625 0 0
CGCAACCTCCTTGC-1 pbmc3k 2310 806 1.5151515 0 0
CGCAACCTGGACGA-1 pbmc3k 2321 820 1.9819044 0 0
CGCACGGAGGACGA-1 pbmc3k 1171 554 2.4765158 3 3
CGCACGGATCTTTG-1 pbmc3k 1930 594 3.2124352 0 0
CGCACTACAGAATG-1 pbmc3k 1864 680 1.2339056 0 0
CGCACTACAGCCAT-1 pbmc3k 3375 1030 1.0370370 2 2
CGCACTACATTGGC-1 pbmc3k 2816 849 1.8110795 2 2
CGCACTACTCGCCT-1 pbmc3k 1281 563 2.0296643 3 3
CGCACTACTCGTGA-1 pbmc3k 2071 879 2.3660068 4 4
CGCACTTGTCACGA-1 pbmc3k 3084 1089 1.7509728 1 1
CGCAGGACAGATCC-1 pbmc3k 2212 822 3.3905967 4 4
CGCAGGACCTACTT-1 pbmc3k 1067 447 1.8744142 0 0
CGCAGGACTTGTCT-1 pbmc3k 5162 1687 2.2084463 5 5
CGCAGGTGCACTGA-1 pbmc3k 2751 865 2.9807343 2 2
CGCAGGTGCCATAG-1 pbmc3k 4314 1252 2.9439036 5 5
CGCAGGTGGGAACG-1 pbmc3k 2276 938 2.8119508 2 2
CGCATAGATCACGA-1 pbmc3k 954 461 1.1530398 1 1
CGCCATACTGCAAC-1 pbmc3k 3446 1053 1.5380151 2 2
CGCCATTGAGAGGC-1 pbmc3k 2763 852 2.2077452 0 0
CGCCATTGCTATGG-1 pbmc3k 1792 737 1.7857143 2 2
CGCCATTGGAGACG-1 pbmc3k 2287 803 1.6615654 2 2
CGCCATTGGAGCAG-1 pbmc3k 2021 772 4.6016823 3 3
CGCCATTGTACTGG-1 pbmc3k 1684 723 1.9002375 3 3
CGCCGAGAGCTTAG-1 pbmc3k 2047 993 1.6121153 6 6
CGCCTAACGAATGA-1 pbmc3k 4597 1455 1.8272787 1 1
CGCCTAACTGCTCC-1 pbmc3k 3489 1006 1.5763829 2 2
CGCGAGACACAGCT-1 pbmc3k 2246 798 3.2502226 2 2
CGCGAGACAGGTCT-1 pbmc3k 3171 1033 3.1535793 3 3
CGCGAGACGCTACA-1 pbmc3k 1968 785 1.6768293 2 2
CGCGATCTCAGTCA-1 pbmc3k 2553 945 1.3317665 1 1
CGCGATCTGTTGAC-1 pbmc3k 2109 742 2.6078710 1 1
CGCGATCTTTCTTG-1 pbmc3k 2394 761 2.3391813 3 3
CGCGGATGGCCAAT-1 pbmc3k 2261 833 1.2826183 2 2
CGCTAAGAATGTCG-1 pbmc3k 2703 1040 0.5549390 2 2
CGCTAAGACAACTG-1 pbmc3k 2771 858 2.0931072 0 0
CGCTAAGACCCTTG-1 pbmc3k 2620 867 2.0229008 0 0
CGCTACTGAACAGA-1 pbmc3k 3806 1186 2.8113505 1 1
CGCTACTGAGAACA-1 pbmc3k 2460 845 1.0162602 0 0
CGCTACTGTGAGCT-1 pbmc3k 1925 686 0.8311688 0 0
CGCTACTGTTCCCG-1 pbmc3k 1366 670 3.1478770 4 4
CGCTCATGCATTTC-1 pbmc3k 1468 628 2.2479564 1 1
CGGAATTGGTTTGG-1 pbmc3k 1896 724 2.2679325 2 2
CGGAATTGTGGAGG-1 pbmc3k 1544 660 0.7124352 2 2
CGGACCGATGCGTA-1 pbmc3k 1834 808 1.3086150 4 4
CGGACCGATGGGAG-1 pbmc3k 985 469 2.6395939 0 0
CGGACTCTAAACAG-1 pbmc3k 1658 599 1.9903498 3 3
CGGACTCTCCAATG-1 pbmc3k 1999 808 2.2011006 2 2
CGGACTCTCCTCGT-1 pbmc3k 1873 854 1.8152696 4 4
CGGAGGCTATTCCT-1 pbmc3k 848 397 4.8349057 0 0
CGGAGGCTTGGATC-1 pbmc3k 2834 784 1.1997177 0 0
CGGATAACAACGAA-1 pbmc3k 2575 892 1.0097087 1 1
CGGATAACAGCTCA-1 pbmc3k 2428 878 1.3179572 2 2
CGGATAACTCAGTG-1 pbmc3k 959 449 2.3983316 1 1
CGGCACGAACTCAG-1 pbmc3k 2270 889 3.6563877 4 4
CGGCACGAAGGGTG-1 pbmc3k 2437 823 3.0365203 0 0
CGGCACGACTACGA-1 pbmc3k 2837 1049 3.4896017 5 5
CGGCATCTTAGAAG-1 pbmc3k 1982 823 2.3208880 4 4
CGGCATCTTCGTAG-1 pbmc3k 3310 1053 1.9939577 1 1
CGGCCAGAAAGGTA-1 pbmc3k 2076 740 2.4084778 0 0
CGGCCAGAGAGGCA-1 pbmc3k 2408 997 2.3255814 4 4
CGGCGAACCAGTCA-1 pbmc3k 3764 1240 2.0988310 1 1
CGGCGAACGACAAA-1 pbmc3k 1587 788 0.8821676 6 6
CGGCGAACGGTCTA-1 pbmc3k 2498 878 1.5612490 2 2
CGGCGAACTACTTC-1 pbmc3k 1729 778 2.0242915 6 6
CGGGACTGCGTGTA-1 pbmc3k 4275 1063 2.5029240 0 0
CGGGACTGGAATAG-1 pbmc3k 2012 814 3.3797217 4 4
CGGGCATGACCCAA-1 pbmc3k 1030 463 4.6601942 0 0
CGGGCATGTCTCTA-1 pbmc3k 2864 1078 2.0600559 4 4
CGGGCATGTTGTGG-1 pbmc3k 2196 742 1.6393443 0 0
CGGTAAACTCGCAA-1 pbmc3k 3142 1095 1.3685551 2 2
CGGTCACTGTTTGG-1 pbmc3k 2138 805 1.8241347 2 2
CGGTCACTTACTTC-1 pbmc3k 3247 1119 1.3242994 2 2
CGTAACGATCGCCT-1 pbmc3k 3413 1022 1.5528860 2 2
CGTACCACACACAC-1 pbmc3k 2084 804 1.7274472 2 2
CGTACCACACGTTG-1 pbmc3k 2157 674 1.4835420 0 0
CGTACCACCTCATT-1 pbmc3k 2382 828 2.2250210 0 0
CGTACCACGGAGCA-1 pbmc3k 2132 763 1.9699812 0 0
CGTACCTGGCATCA-1 pbmc3k 2472 996 2.5080906 6 6
CGTAGCCTCTCTCG-1 pbmc3k 2310 851 2.0346320 1 1
CGTAGCCTGCGAAG-1 pbmc3k 2242 866 1.2042819 2 2
CGTAGCCTGTATGC-1 pbmc3k 3497 1116 4.1178153 1 1
CGTCAAGAAAGGTA-1 pbmc3k 1111 505 2.4302430 1 1
CGTCAAGAACGTGT-1 pbmc3k 2815 927 1.5630551 0 0
CGTCAAGACAGAGG-1 pbmc3k 2748 956 1.9286754 2 2
CGTCAAGACAGGAG-1 pbmc3k 2162 931 1.9888992 4 4
CGTCCATGCTCTTA-1 pbmc3k 2746 892 1.3109978 0 0
CGTCGACTTTCCGC-1 pbmc3k 2188 903 1.6453382 4 4
CGTGATGACGCTAA-1 pbmc3k 909 494 1.8701870 0 0
CGTGATGAGGTTCA-1 pbmc3k 1747 708 3.2054951 4 4
CGTGCACTTATGGC-1 pbmc3k 3067 1064 1.5324421 1 1
CGTGTAGAAAAACG-1 pbmc3k 2027 794 2.1706956 2 2
CGTGTAGACGATAC-1 pbmc3k 1650 783 1.4545455 4 4
CGTGTAGAGTTACG-1 pbmc3k 2074 950 1.7839923 6 6
CGTGTAGAGTTCAG-1 pbmc3k 1889 719 1.7469561 1 1
CGTGTAGATTCGGA-1 pbmc3k 1674 842 1.6129032 6 6
CGTTAGGAAACCAC-1 pbmc3k 2240 888 2.0089286 2 2
CGTTAGGATCATTC-1 pbmc3k 4080 1351 1.2254902 1 1
CGTTATACCCTGAA-1 pbmc3k 2386 1002 3.2690696 4 4
CGTTTAACTGGTCA-1 pbmc3k 2767 872 1.4094687 2 2
CTAAACCTCTGACA-1 pbmc3k 2532 1184 3.6334913 6 6
CTAAACCTGTGCAT-1 pbmc3k 2516 909 1.4308426 3 3
CTAACACTAACGTC-1 pbmc3k 2764 869 3.7626628 2 2
CTAACACTAGTGCT-1 pbmc3k 2355 894 2.2929936 1 1
CTAACGGAACCGAT-1 pbmc3k 1997 995 1.5523285 6 6
CTAACGGATTTCTG-1 pbmc3k 2256 769 1.3741135 0 0
CTAACTACGGCAAG-1 pbmc3k 1286 553 1.2441680 0 0
CTAAGGACACCATG-1 pbmc3k 2134 805 2.6710403 0 0
CTAAGGACCGTTAG-1 pbmc3k 5484 1425 1.7505470 2 2
CTAAGGACGCCATA-1 pbmc3k 2878 1011 1.9805420 1 1
CTAAGGTGCCTAAG-1 pbmc3k 1111 452 1.8001800 3 3
CTAAGGTGTTGCAG-1 pbmc3k 2957 894 3.8214406 2 2
CTAAGGTGTTTCTG-1 pbmc3k 1009 426 0.8919722 0 0
CTAATAGAGCTATG-1 pbmc3k 600 239 0.1666667 0 0
CTAATGCTTGTGGT-1 pbmc3k 2067 829 2.8543783 1 1
CTACAACTCCCGTT-1 pbmc3k 2194 895 2.0510483 4 4
CTACCTCTCAACCA-1 pbmc3k 2333 786 0.8144021 2 2
CTACGCACACCTAG-1 pbmc3k 3710 1136 2.5067385 5 5
CTACGCACTCTCCG-1 pbmc3k 2637 923 1.3272658 2 2
CTACGCACTGGTCA-1 pbmc3k 2468 1012 2.0664506 6 6
CTACGGCTTTCTTG-1 pbmc3k 3911 1357 1.6619790 1 1
CTACTATGAACCAC-1 pbmc3k 3184 1093 2.4183417 1 1
CTACTATGATGTGC-1 pbmc3k 1662 649 2.3465704 1 1
CTACTATGCTAAGC-1 pbmc3k 2704 851 2.4038462 2 2
CTACTATGTAAAGG-1 pbmc3k 1264 548 2.5316456 3 3
CTACTCCTATGTCG-1 pbmc3k 2181 1052 1.8340211 6 6
CTACTCCTGCCATA-1 pbmc3k 586 307 3.7542662 1 1
CTAGAGACACTTTC-1 pbmc3k 3199 1150 1.9068459 2 2
CTAGAGACAGCATC-1 pbmc3k 2206 907 0.8159565 4 4
CTAGAGACTTTGGG-1 pbmc3k 5515 1621 3.3544878 5 5
CTAGATCTCTCTAT-1 pbmc3k 1822 731 1.8111965 2 2
CTAGATCTTCGACA-1 pbmc3k 2392 897 1.4214047 2 2
CTAGGATGAGCCTA-1 pbmc3k 5867 1741 1.5680927 3 3
CTAGGATGATCGTG-1 pbmc3k 2159 762 1.7137564 3 3
CTAGGCCTCTCAGA-1 pbmc3k 1998 712 0.2002002 2 2
CTAGGTGATGGTTG-1 pbmc3k 3254 1195 1.9360787 5 5
CTAGTTACCAGAGG-1 pbmc3k 2740 850 1.9343066 0 0
CTAGTTACCGCATA-1 pbmc3k 2357 861 2.2061943 2 2
CTAGTTACGAAACA-1 pbmc3k 2245 966 2.2717149 6 6
CTATAAGATCGTTT-1 pbmc3k 2733 843 1.0611050 3 3
CTATACTGAGGTTC-1 pbmc3k 732 331 0.4098361 1 1
CTATACTGCCAGTA-1 pbmc3k 2691 839 2.1181717 0 0
CTATACTGCGCTAA-1 pbmc3k 1856 733 1.6163793 1 1
CTATACTGCTACGA-1 pbmc3k 2129 947 1.8788163 6 6
CTATACTGTCTCAT-1 pbmc3k 1774 842 4.0022548 6 6
CTATACTGTTCGTT-1 pbmc3k 4760 1520 1.2394958 0 0
CTATAGCTGTCACA-1 pbmc3k 2123 660 1.9783325 0 0
CTATAGCTTCGCTC-1 pbmc3k 2542 980 2.7537372 1 1
CTATAGCTTGCCTC-1 pbmc3k 2498 912 3.4027222 1 1
CTATCAACGAACTC-1 pbmc3k 2520 910 2.1428571 0 0
CTATCAACGCAGAG-1 pbmc3k 3160 1060 2.2468354 2 2
CTATCAACTTTGGG-1 pbmc3k 2053 679 3.3609352 0 0
CTATCCCTCCACCT-1 pbmc3k 2172 822 2.2099448 1 1
CTATGTACGAGAGC-1 pbmc3k 3144 995 1.1768448 2 2
CTATGTACGCTTAG-1 pbmc3k 2072 693 1.9305019 0 0
CTATGTACTGTTTC-1 pbmc3k 4824 1481 1.2023217 3 3
CTATGTTGAAAGCA-1 pbmc3k 3166 925 1.9583070 2 2
CTATGTTGTCCTCG-1 pbmc3k 1635 574 1.4067278 0 0
CTATGTTGTCTCGC-1 pbmc3k 3493 1051 2.4334383 2 2
CTATTGACAAACGA-1 pbmc3k 1289 571 4.2668735 1 1
CTATTGACACTGGT-1 pbmc3k 2939 1027 2.2116366 1 1
CTATTGACGGTGAG-1 pbmc3k 3967 1262 1.6133098 1 1
CTATTGTGGCAAGG-1 pbmc3k 1937 669 3.6138358 0 0
CTCAATTGGTTCAG-1 pbmc3k 3235 956 1.3601236 2 2
CTCAATTGGTTGCA-1 pbmc3k 1907 746 3.1463031 1 1
CTCAGAGATAGAAG-1 pbmc3k 2565 863 1.3645224 0 0
CTCAGCACTCTAGG-1 pbmc3k 2343 895 2.0059752 2 2
CTCAGCACTGAACC-1 pbmc3k 1151 488 1.4769765 1 1
CTCAGCACTTGCAG-1 pbmc3k 1860 643 1.5591398 0 0
CTCAGCTGAACCTG-1 pbmc3k 2927 922 2.5623505 1 1
CTCAGCTGCAGTTG-1 pbmc3k 2341 819 2.4775737 0 0
CTCAGGCTCGTTGA-1 pbmc3k 4502 1463 2.4655709 5 5
CTCAGGCTGCTAAC-1 pbmc3k 1197 549 1.6708438 0 0
CTCATTGACCTTAT-1 pbmc3k 3103 1112 0.6767644 2 2
CTCATTGATGCTTT-1 pbmc3k 3006 1005 2.1290752 5 5
CTCCACGAGAGATA-1 pbmc3k 4633 1445 2.0289229 7 7
CTCCATCTCTTAGG-1 pbmc3k 2107 725 1.1865211 0 0
CTCCATCTGACGAG-1 pbmc3k 2886 898 1.5246015 0 0
CTCCGAACAAGTGA-1 pbmc3k 2439 992 2.2550226 5 5
CTCCTACTGCCTTC-1 pbmc3k 1292 563 3.7151703 1 1
CTCGAAGATGTGGT-1 pbmc3k 2951 869 2.3043036 0 0
CTCGAAGATTAGGC-1 pbmc3k 2373 903 0.9692373 1 1
CTCGACTGCTCTAT-1 pbmc3k 1143 515 1.0498688 0 0
CTCGACTGGGTGAG-1 pbmc3k 2020 751 1.7326733 0 0
CTCGACTGGTTGAC-1 pbmc3k 1810 875 2.2651934 6 6
CTCGAGCTCTGGAT-1 pbmc3k 1157 588 0.9507347 4 4
CTCGCATGACTTTC-1 pbmc3k 1839 740 4.2958129 4 4
CTCGCATGCTTAGG-1 pbmc3k 2188 796 3.5648995 0 0
CTCTAAACCTCGAA-1 pbmc3k 2271 797 1.0568032 0 0
CTCTAAACGGCGAA-1 pbmc3k 2001 651 1.7491254 0 0
CTCTAATGTCCAAG-1 pbmc3k 2338 901 2.9940120 1 1
CTGAACGACAGTCA-1 pbmc3k 2728 986 0.8797654 2 2
CTGAACGATGAGGG-1 pbmc3k 1537 723 2.3422251 2 2
CTGAAGACCCAACA-1 pbmc3k 2752 952 2.6889535 2 2
CTGAAGACGTGCAT-1 pbmc3k 2300 891 2.5652174 3 3
CTGAAGTGAAGCCT-1 pbmc3k 4070 1434 2.9975430 5 5
CTGAAGTGCAGCTA-1 pbmc3k 1714 650 1.3418903 3 3
CTGAAGTGGCTATG-1 pbmc3k 1889 712 0.8470090 0 0
CTGAAGTGTCCAGA-1 pbmc3k 3299 955 0.6062443 0 0
CTGAATCTGAATAG-1 pbmc3k 2022 876 1.8793274 2 2
CTGACAGAATCGTG-1 pbmc3k 2698 1010 1.0378058 4 4
CTGACCACAGCAAA-1 pbmc3k 2477 823 0.8074283 0 0
CTGAGAACCGGGAA-1 pbmc3k 3915 1076 2.0689655 2 2
CTGAGAACGTAAAG-1 pbmc3k 1418 738 1.7630465 6 6
CTGATACTAGTAGA-1 pbmc3k 2228 691 1.6157989 0 0
CTGATTTGGTGTTG-1 pbmc3k 2992 1061 1.1697861 1 1
CTGCAGCTAACCGT-1 pbmc3k 2674 771 3.4031414 0 0
CTGCAGCTGACACT-1 pbmc3k 3101 807 0.7094486 0 0
CTGCAGCTGGATTC-1 pbmc3k 4043 1386 1.5582488 5 5
CTGCAGCTTGGCAT-1 pbmc3k 3900 1264 2.3076923 4 4
CTGCCAACAGGAGC-1 pbmc3k 2857 952 2.9751488 2 2
CTGCCAACCAGCTA-1 pbmc3k 2209 814 1.2675419 0 0
CTGCCAACTAACCG-1 pbmc3k 2262 793 2.0335986 1 1
CTGCCAACTGCTCC-1 pbmc3k 1869 794 2.7287319 4 4
CTGCCAACTTGCAG-1 pbmc3k 2344 760 3.2423208 0 0
CTGCCAACTTGCTT-1 pbmc3k 2327 882 2.2776107 4 4
CTGCGACTCCACCT-1 pbmc3k 687 348 3.4934498 0 0
CTGGAAACAAACGA-1 pbmc3k 2367 868 2.8728348 4 4
CTGGAAACATCGAC-1 pbmc3k 2151 842 1.3947001 0 0
CTGGATGACTGGAT-1 pbmc3k 2036 818 2.7996071 4 4
CTGGATGACTTGTT-1 pbmc3k 1455 624 4.1237113 2 2
CTGGATGATGTGAC-1 pbmc3k 868 417 1.6129032 3 3
CTGGCACTCAAGCT-1 pbmc3k 2563 901 1.9508389 1 1
CTGTAACTAACCAC-1 pbmc3k 2165 836 2.9561201 2 2
CTGTAACTAGCGTT-1 pbmc3k 1982 722 0.9586276 0 0
CTGTATACGTAAAG-1 pbmc3k 979 436 2.0429009 0 0
CTGTATACGTACGT-1 pbmc3k 3500 1108 3.4571429 1 1
CTGTATACGTTGGT-1 pbmc3k 1827 723 0.8757526 0 0
CTGTGAGACAACCA-1 pbmc3k 4058 1298 2.2917693 5 5
CTGTGAGACCTTGC-1 pbmc3k 1660 717 4.2168675 4 4
CTGTGAGACGAACT-1 pbmc3k 2277 843 1.3614405 1 1
CTGTGAGACTGTAG-1 pbmc3k 3255 1089 2.3655914 1 1
CTTAAAGAACCTGA-1 pbmc3k 2297 696 1.5672616 0 0
CTTAACACCTGTAG-1 pbmc3k 2068 885 1.6924565 0 0
CTTAACACTATCGG-1 pbmc3k 1748 627 2.2883295 3 3
CTTAAGCTACCTAG-1 pbmc3k 2360 807 2.0762712 0 0
CTTAAGCTAGTACC-1 pbmc3k 920 423 1.7391304 0 0
CTTAAGCTCATCAG-1 pbmc3k 1860 729 3.0645161 0 0
CTTAAGCTCCGCTT-1 pbmc3k 2372 851 2.9932546 3 3
CTTACAACTAACGC-1 pbmc3k 2368 854 1.7314189 0 0
CTTACAACTCCCGT-1 pbmc3k 1504 607 1.1968085 1 1
CTTACTGACGTACA-1 pbmc3k 1857 627 2.2078621 0 0
CTTAGACTAAACGA-1 pbmc3k 2396 823 1.2938230 2 2
CTTAGGGACTTGCC-1 pbmc3k 2989 1061 0.6691201 2 2
CTTAGGGAGAATCC-1 pbmc3k 1636 573 2.3227384 0 0
CTTATCGACTCATT-1 pbmc3k 1966 743 1.9328586 2 2
CTTCACCTACCTGA-1 pbmc3k 4888 1528 2.3936170 5 5
CTTCATGAAGCATC-1 pbmc3k 1927 681 1.7644006 3 3
CTTCATGAAGTACC-1 pbmc3k 1322 542 1.8910741 0 0
CTTCATGACCGAAT-1 pbmc3k 935 420 3.5294118 0 0
CTTGAACTACGCAT-1 pbmc3k 2077 731 1.6369764 0 0
CTTGATTGAGGTTC-1 pbmc3k 2005 920 1.6957606 4 4
CTTGATTGATCTTC-1 pbmc3k 2094 761 2.5310411 1 1
CTTGATTGCATTCT-1 pbmc3k 2273 881 2.9476463 2 2
CTTGATTGTTTCGT-1 pbmc3k 3234 981 1.1131725 2 2
CTTGTATGACACCA-1 pbmc3k 2020 784 1.2871287 3 3
CTTGTATGCGCAAT-1 pbmc3k 1035 496 1.9323671 3 3
CTTTACGAGCGAAG-1 pbmc3k 1483 573 4.0458530 0 0
CTTTAGACCGTGAT-1 pbmc3k 1507 521 2.5215660 0 0
CTTTAGACGAGACG-1 pbmc3k 2250 662 2.0888889 0 0
CTTTAGACGATACC-1 pbmc3k 2498 792 1.1609287 0 0
CTTTAGACGTTGGT-1 pbmc3k 2934 972 3.3060668 4 4
CTTTAGACTCATTC-1 pbmc3k 2510 855 3.2270916 4 4
CTTTAGTGACGGGA-1 pbmc3k 2214 978 2.0325203 6 6
CTTTAGTGGGTGGA-1 pbmc3k 1648 590 1.5776699 0 0
CTTTCAGAGAAACA-1 pbmc3k 2493 902 1.3638187 0 0
CTTTGATGAGCACT-1 pbmc3k 2019 694 1.3868252 0 0
CTTTGATGTCTAGG-1 pbmc3k 1935 816 2.9457364 4 4
CTTTGATGTGTCCC-1 pbmc3k 3460 974 2.3988439 2 2
CTTTGATGTGTGGT-1 pbmc3k 2490 816 1.6465863 0 0
GAAACAGAACTACG-1 pbmc3k 1958 751 1.3789581 0 0
GAAACAGAATCACG-1 pbmc3k 2178 789 2.0202020 0 0
GAAACAGACATTCT-1 pbmc3k 2572 858 1.5552100 0 0
GAAACCTGATCGTG-1 pbmc3k 2487 813 1.3671090 0 0
GAAACCTGATGCCA-1 pbmc3k 2390 841 2.2175732 2 2
GAAACCTGCTTATC-1 pbmc3k 3634 1080 1.0181618 2 2
GAAACCTGGACTAC-1 pbmc3k 5326 1690 2.5910627 5 5
GAAACCTGTGCTAG-1 pbmc3k 1723 716 1.6831109 2 2
GAAAGATGATTTCC-1 pbmc3k 2501 955 1.2395042 2 2
GAAAGATGCTGATG-1 pbmc3k 4688 1464 2.3250853 5 5
GAAAGATGCTTCGC-1 pbmc3k 2315 740 0.9935205 1 1
GAAAGATGTAAGGA-1 pbmc3k 650 295 4.1538462 0 0
GAAAGCCTACGTTG-1 pbmc3k 2282 738 1.8404908 3 3
GAAAGTGAAAGTGA-1 pbmc3k 6175 1571 2.5910931 7 7
GAAAGTGACCACAA-1 pbmc3k 3254 1118 2.6429010 1 1
GAAAGTGACTCAAG-1 pbmc3k 3358 917 2.5312686 2 2
GAAATACTACCAAC-1 pbmc3k 561 283 1.6042781 1 1
GAAATACTCTTAGG-1 pbmc3k 2037 701 2.3564065 3 3
GAAATACTTCCTCG-1 pbmc3k 2034 840 1.6715831 2 2
GAACACACGTGCAT-1 pbmc3k 3826 1251 2.4307371 1 1
GAACACACTGCCTC-1 pbmc3k 2166 674 4.0627886 0 0
GAACAGCTAACTGC-1 pbmc3k 5489 1631 2.3319366 5 5
GAACAGCTCTCAGA-1 pbmc3k 4199 1273 2.9530841 5 5
GAACCAACCACAAC-1 pbmc3k 863 380 1.5063731 0 0
GAACCAACTTCCGC-1 pbmc3k 1607 796 2.0535159 6 6
GAACCTGAACGTGT-1 pbmc3k 4754 1489 3.1973075 2 2
GAACCTGAGAGACG-1 pbmc3k 2490 878 0.8032129 2 2
GAACCTGATGAACC-1 pbmc3k 2869 937 1.2896480 2 2
GAACGGGATACTTC-1 pbmc3k 2033 725 1.4756517 0 0
GAACTGTGACCTGA-1 pbmc3k 4023 1361 2.0134228 5 5
GAACTGTGCCAGTA-1 pbmc3k 2040 818 1.8137255 1 1
GAAGAATGCAATCG-1 pbmc3k 2089 718 2.2498803 0 0
GAAGCGGACCTATT-1 pbmc3k 4006 1341 2.2965552 1 1
GAAGCTACGAATGA-1 pbmc3k 2607 902 2.1864212 1 1
GAAGCTACGGTTTG-1 pbmc3k 1763 837 1.8150879 2 2
GAAGGGTGAAAGTG-1 pbmc3k 2472 802 0.8090615 2 2
GAAGGGTGCTTAGG-1 pbmc3k 2937 988 2.4174328 1 1
GAAGGTCTGAAAGT-1 pbmc3k 2278 1082 0.9657594 2 2
GAAGGTCTGTTGCA-1 pbmc3k 3210 973 1.2772586 0 0
GAAGGTCTTAAAGG-1 pbmc3k 5637 1627 1.6852936 7 7
GAAGTAGACTCCCA-1 pbmc3k 1871 795 2.3516836 4 4
GAAGTAGATCCAAG-1 pbmc3k 4035 1408 2.6517968 5 5
GAAGTCACCCTCGT-1 pbmc3k 1853 771 1.5650297 1 1
GAAGTCACCCTGTC-1 pbmc3k 1975 647 2.3797468 3 3
GAAGTCTGTCGCAA-1 pbmc3k 1998 779 2.1021021 3 3
GAAGTCTGTTCTGT-1 pbmc3k 2242 646 2.4977698 0 0
GAAGTGCTAAACGA-1 pbmc3k 1804 815 2.9379157 6 6
GAAGTGCTCCGCTT-1 pbmc3k 3251 1127 2.7991387 1 1
GAAGTGCTTAACCG-1 pbmc3k 2586 881 2.2815159 2 2
GAATGCACCCTAAG-1 pbmc3k 3034 950 1.6809492 2 2
GAATGCACCTTCGC-1 pbmc3k 1978 828 2.0222447 6 6
GAATGCTGCGGTAT-1 pbmc3k 2569 732 3.8925652 0 0
GAATTAACGATAAG-1 pbmc3k 2135 782 1.2646370 0 0
GAATTAACGGTCAT-1 pbmc3k 1911 753 1.7268446 0 0
GAATTAACGTCGTA-1 pbmc3k 1319 705 1.5163002 6 6
GAATTAACTGAAGA-1 pbmc3k 2480 975 2.0967742 3 3
GACAACACAGGCGA-1 pbmc3k 2576 716 1.5916149 0 0
GACAACACATCGTG-1 pbmc3k 2592 887 2.1990741 0 0
GACAACACTCGCCT-1 pbmc3k 2228 808 1.5709156 0 0
GACAACTGAGGTTC-1 pbmc3k 2501 795 1.9992003 2 2
GACAGGGAAGAGTA-1 pbmc3k 1573 661 2.8607756 3 3
GACAGGGAATGCCA-1 pbmc3k 2541 911 1.1806375 2 2
GACAGTACGAGCTT-1 pbmc3k 4929 1546 2.4345709 5 5
GACAGTACTTCGGA-1 pbmc3k 5193 1509 4.7949162 5 5
GACAGTTGAGTAGA-1 pbmc3k 3107 1021 1.5770840 3 3
GACATTCTCCACCT-1 pbmc3k 6191 1749 2.5036343 7 7
GACCAAACGACTAC-1 pbmc3k 2041 774 3.9196472 1 1
GACCAAACGTATCG-1 pbmc3k 2228 910 2.9622980 1 1
GACCCTACTAAAGG-1 pbmc3k 1493 714 1.4735432 3 3
GACCTAGACCTCAC-1 pbmc3k 1177 529 4.9277825 0 0
GACCTAGACGAGAG-1 pbmc3k 2591 846 4.3226553 2 2
GACCTCACAAGGTA-1 pbmc3k 1822 628 1.5916575 0 0
GACCTCACGTACGT-1 pbmc3k 3514 1152 2.2766079 1 1
GACCTCTGCATCAG-1 pbmc3k 4014 1118 2.1923269 0 0
GACGAACTCCCACT-1 pbmc3k 2191 755 1.4605203 0 0
GACGATTGCCAATG-1 pbmc3k 1183 570 1.5215554 3 3
GACGCCGACCTTCG-1 pbmc3k 2707 1175 1.7362394 2 2
GACGCTCTCTCTCG-1 pbmc3k 682 266 3.2258065 8 8
GACGGCACACGGGA-1 pbmc3k 1800 915 1.0000000 6 6
GACGGCACGAGATA-1 pbmc3k 3152 1130 2.2208122 1 1
GACGTAACCTATGG-1 pbmc3k 2218 849 1.3976555 0 0
GACGTAACCTGTGA-1 pbmc3k 2520 937 1.6666667 2 2
GACGTAACTATGGC-1 pbmc3k 3217 1138 1.7718371 2 2
GACGTATGTTGACG-1 pbmc3k 2551 906 0.9016072 0 0
GACGTATGTTTGCT-1 pbmc3k 2409 839 1.1207970 0 0
GACGTCCTACGGAG-1 pbmc3k 2223 803 1.6644175 2 2
GACGTCCTCTCAAG-1 pbmc3k 2411 902 2.6545002 1 1
GACGTCCTGATAAG-1 pbmc3k 2255 787 1.5964523 3 3
GACTACGATGGTCA-1 pbmc3k 1872 776 1.7094017 3 3
GACTCCTGCTCGCT-1 pbmc3k 1861 714 3.4390113 0 0
GACTCCTGGGTTAC-1 pbmc3k 2647 867 2.5689460 0 0
GACTCCTGTTATCC-1 pbmc3k 1462 714 2.3255814 2 2
GACTCCTGTTGGTG-1 pbmc3k 2716 879 2.0618557 1 1
GACTGAACCAATCG-1 pbmc3k 2297 763 1.1319112 1 1
GACTGATGTGATGC-1 pbmc3k 2647 855 1.3600302 0 0
GACTTTACATGCCA-1 pbmc3k 2475 784 0.9292929 0 0
GACTTTACGACAGG-1 pbmc3k 1349 552 1.5567087 3 3
GAGAAATGTTCTCA-1 pbmc3k 2055 633 3.2116788 3 3
GAGATAGAAAAAGC-1 pbmc3k 3323 1226 1.6551309 1 1
GAGATCACGACAAA-1 pbmc3k 3174 1131 2.0163831 5 5
GAGATGCTCTGGAT-1 pbmc3k 2074 848 2.7965284 4 4
GAGATGCTGAATGA-1 pbmc3k 1802 809 1.7758047 2 2
GAGCAGGATTCCCG-1 pbmc3k 1896 823 1.7405063 4 4
GAGCATACTTTGCT-1 pbmc3k 6691 1750 1.6738903 5 5
GAGCGCACGCGTAT-1 pbmc3k 1774 710 2.7621195 1 1
GAGCGCACGGTGAG-1 pbmc3k 1054 482 4.6489564 4 4
GAGCGCTGAAGATG-1 pbmc3k 1630 782 2.8834356 6 6
GAGCGCTGTCTTAC-1 pbmc3k 1978 721 1.4661274 1 1
GAGCGGCTGGGAGT-1 pbmc3k 1519 595 2.4358130 3 3
GAGGACGACTCAGA-1 pbmc3k 1171 514 1.2809564 0 0
GAGGATCTGAAAGT-1 pbmc3k 716 371 0.4189944 3 3
GAGGCAGACTTGCC-1 pbmc3k 2237 923 1.2069736 4 4
GAGGGAACACCAGT-1 pbmc3k 1676 746 0.5966587 2 2
GAGGGAACGAGGGT-1 pbmc3k 1827 840 2.3535851 2 2
GAGGGATGGGAAAT-1 pbmc3k 1496 672 2.0053476 0 0
GAGGGCCTTCACCC-1 pbmc3k 2479 954 2.7027027 2 2
GAGGGTGAAGAGTA-1 pbmc3k 2598 795 1.5396459 0 0
GAGGTACTACGGTT-1 pbmc3k 2041 719 3.0377266 0 0
GAGGTACTACTCAG-1 pbmc3k 1979 717 2.0212228 1 1
GAGGTACTGACACT-1 pbmc3k 1922 857 1.3527575 6 6
GAGGTACTGGGAGT-1 pbmc3k 1321 578 0.8327025 0 0
GAGGTACTTAGCGT-1 pbmc3k 3385 1062 2.8360414 1 1
GAGGTGGAGTACGT-1 pbmc3k 1514 593 4.0951123 0 0
GAGGTGGATCCTCG-1 pbmc3k 1988 997 1.9617706 6 6
GAGGTTACTCGTTT-1 pbmc3k 3753 1264 1.2523315 1 1
GAGGTTTGTAAGCC-1 pbmc3k 684 341 3.3625731 1 1
GAGTCAACCATTCT-1 pbmc3k 897 388 1.7837235 0 0
GAGTCAACGGGAGT-1 pbmc3k 2625 751 1.9047619 0 0
GAGTCTGATCGTGA-1 pbmc3k 2349 829 1.2771392 2 2
GAGTCTGATTTGGG-1 pbmc3k 1492 592 2.0777480 3 3
GAGTGACTCAGCTA-1 pbmc3k 2883 1185 1.6302463 2 2
GAGTGACTCGGTAT-1 pbmc3k 2182 910 1.5123740 4 4
GAGTGACTCTTGCC-1 pbmc3k 1833 738 1.5275505 3 3
GAGTGACTGACTAC-1 pbmc3k 1253 473 4.0702314 3 3
GAGTGACTGTCTAG-1 pbmc3k 2146 816 1.9571295 0 0
GAGTGGGAGTCTTT-1 pbmc3k 1580 640 2.5316456 3 3
GAGTGGGATGCCCT-1 pbmc3k 2473 744 2.0622725 0 0
GAGTGGGATGCTGA-1 pbmc3k 1497 634 1.9372077 3 3
GAGTGTTGCTGTAG-1 pbmc3k 2788 951 2.0803443 2 2
GAGTGTTGTGGTCA-1 pbmc3k 2043 800 1.6152717 0 0
GAGTTGTGCATGGT-1 pbmc3k 692 325 2.7456647 1 1
GAGTTGTGCTGAGT-1 pbmc3k 2000 849 2.3000000 4 4
GAGTTGTGGCGAGA-1 pbmc3k 1224 498 1.2254902 1 1
GAGTTGTGGTAGCT-1 pbmc3k 2405 849 0.9147609 8 8
GAGTTGTGTATGCG-1 pbmc3k 4175 1416 2.5389222 5 5
GATAAGGAGAAACA-1 pbmc3k 1138 486 3.0755712 1 1
GATAAGGATTCACT-1 pbmc3k 2517 875 2.7810886 1 1
GATACTCTATCGGT-1 pbmc3k 2341 795 1.6659547 0 0
GATACTCTTACTTC-1 pbmc3k 2879 979 2.8829455 0 0
GATACTCTTGACTG-1 pbmc3k 2142 752 2.6143791 1 1
GATAGAGAAGGGTG-1 pbmc3k 2063 850 3.1022782 6 6
GATAGAGACTGTGA-1 pbmc3k 3962 1095 0.9591116 0 0
GATAGAGATCACGA-1 pbmc3k 2808 995 1.4245014 1 1
GATAGCACCCATAG-1 pbmc3k 2636 827 2.0864947 0 0
GATAGCACGAAGGC-1 pbmc3k 3628 1157 1.9570011 1 1
GATAGCACTTGTCT-1 pbmc3k 2941 954 2.0401224 0 0
GATATAACAAGGTA-1 pbmc3k 3232 1059 1.5779703 2 2
GATATAACACGCAT-1 pbmc3k 2246 695 0.7123776 0 0
GATATATGCTGGAT-1 pbmc3k 2668 786 2.2488756 0 0
GATATATGTCCGTC-1 pbmc3k 1819 743 1.4843321 4 4
GATATATGTGGAGG-1 pbmc3k 3773 1209 2.5178903 1 1
GATATCCTAGAAGT-1 pbmc3k 2301 892 1.3037810 0 0
GATATCCTCCCGTT-1 pbmc3k 1070 490 1.3084112 1 1
GATATTGACAGGAG-1 pbmc3k 1735 743 3.6311239 4 4
GATATTGACGAGTT-1 pbmc3k 4409 1446 1.3608528 5 5
GATATTGAGCCAAT-1 pbmc3k 2421 764 1.4869888 0 0
GATCCCTGACCTTT-1 pbmc3k 2213 1050 1.0845007 6 6
GATCCCTGTGTAGC-1 pbmc3k 2322 851 1.4642550 4 4
GATCCGCTGGTCAT-1 pbmc3k 3066 1114 1.7286367 1 1
GATCGAACCGAGAG-1 pbmc3k 4587 1425 2.4852845 2 2
GATCGATGACTAGC-1 pbmc3k 1136 554 0.8802817 3 3
GATCGATGGTAAAG-1 pbmc3k 1771 612 1.8633540 3 3
GATCGATGTAAGGA-1 pbmc3k 2504 854 1.2779553 1 1
GATCGTGACACTAG-1 pbmc3k 2249 786 1.7341040 0 0
GATCGTGATTCACT-1 pbmc3k 2068 889 1.0638298 2 2
GATCTACTGGTGAG-1 pbmc3k 1967 782 2.6944586 2 2
GATCTACTTTGCAG-1 pbmc3k 1624 712 1.1699507 3 3
GATCTTACACCCAA-1 pbmc3k 3608 1323 2.5776053 5 5
GATCTTACCCTACC-1 pbmc3k 2802 920 2.4268380 2 2
GATCTTACGAATAG-1 pbmc3k 1541 681 2.0765737 1 1
GATCTTACGAGATA-1 pbmc3k 2197 790 2.6854802 1 1
GATGCAACTCCAGA-1 pbmc3k 2486 845 0.8849558 0 0
GATGCCCTACGTAC-1 pbmc3k 2808 1010 2.5284900 2 2
GATGCCCTCTCATT-1 pbmc3k 2402 838 1.3738551 0 0
GATGCCCTGGCAAG-1 pbmc3k 2299 806 1.0004350 1 1
GATGCCCTTTTGCT-1 pbmc3k 1522 584 3.6136662 0 0
GATTACCTTGTTCT-1 pbmc3k 2468 878 1.9854133 0 0
GATTCGGAACGACT-1 pbmc3k 2623 779 2.4780785 0 0
GATTCGGACAGGAG-1 pbmc3k 2871 1008 1.6370603 1 1
GATTCGGAGAAGGC-1 pbmc3k 3633 1181 2.3671897 5 5
GATTCTTGATTCGG-1 pbmc3k 3001 1149 1.5994668 1 1
GATTCTTGCCGATA-1 pbmc3k 2258 808 2.0372011 0 0
GATTCTTGCGAGTT-1 pbmc3k 1547 604 2.7149321 3 3
GATTGGACCCGTTC-1 pbmc3k 3465 1155 1.2987013 3 3
GATTGGACGGTGTT-1 pbmc3k 1759 714 3.1836271 3 3
GATTGGACTTTCGT-1 pbmc3k 1632 640 2.0220588 3 3
GATTGGTGTGTCAG-1 pbmc3k 3235 1087 1.1746522 2 2
GATTTAGACACTCC-1 pbmc3k 4119 1290 3.1561059 5 5
GATTTAGACTAAGC-1 pbmc3k 2964 1193 1.3832659 3 3
GATTTAGATTCGTT-1 pbmc3k 662 323 1.5105740 1 1
GATTTGCTAACGAA-1 pbmc3k 2322 804 1.8087855 0 0
GATTTGCTAACGGG-1 pbmc3k 691 370 1.1577424 1 1
GCAACCCTCCTCGT-1 pbmc3k 744 359 1.6129032 1 1
GCAACTGATTGCGA-1 pbmc3k 2803 853 2.6400285 0 0
GCAAGACTACTGGT-1 pbmc3k 2894 1165 0.6219765 2 2
GCAAGACTAGGTCT-1 pbmc3k 2191 836 2.8297581 2 2
GCAAGACTCCCTTG-1 pbmc3k 2701 923 2.0733062 0 0
GCAATCGACTGCAA-1 pbmc3k 2344 861 3.5409556 2 2
GCAATCGAGACGTT-1 pbmc3k 3898 1283 3.0528476 1 1
GCAATCGATCCTTA-1 pbmc3k 3053 1010 2.1618081 1 1
GCAATTCTCGTGTA-1 pbmc3k 2592 889 1.8904321 2 2
GCAATTCTTCTCCG-1 pbmc3k 3261 983 1.8092610 2 2
GCACAAACAATGCC-1 pbmc3k 1238 510 3.4733441 0 0
GCACAAACGGTACT-1 pbmc3k 2829 939 1.1311417 2 2
GCACAATGGTGCAT-1 pbmc3k 2610 881 1.1877395 1 1
GCACACCTGTGCTA-1 pbmc3k 1374 695 2.1834061 6 6
GCACCACTCATGAC-1 pbmc3k 3641 1208 1.5929690 1 1
GCACCACTGTTTGG-1 pbmc3k 776 362 1.5463918 1 1
GCACCACTTCCTTA-1 pbmc3k 1945 969 2.9305913 6 6
GCACCACTTTCGGA-1 pbmc3k 3246 1135 1.7560074 1 1
GCACCTACGCGATT-1 pbmc3k 4271 1236 1.1004449 2 2
GCACCTTGGCTGTA-1 pbmc3k 1753 580 1.3690816 0 0
GCACCTTGGGGAGT-1 pbmc3k 3739 1102 1.9523937 1 1
GCACGGACCAGCTA-1 pbmc3k 3169 972 1.6408962 2 2
GCACGGTGACCTCC-1 pbmc3k 2261 824 1.1941619 2 2
GCACGGTGCTATGG-1 pbmc3k 3103 1168 2.5781502 1 1
GCACTAGAACGGGA-1 pbmc3k 2491 919 2.5291048 2 2
GCACTAGAAGATGA-1 pbmc3k 1545 637 2.4595469 3 3
GCACTAGACCTTTA-1 pbmc3k 3626 1176 2.3993381 5 5
GCACTAGACGTAAC-1 pbmc3k 743 376 1.8842530 3 3
GCACTAGAGTCGTA-1 pbmc3k 2419 977 2.3563456 4 4
GCACTAGATGCAAC-1 pbmc3k 2664 913 1.3888889 0 0
GCACTGCTGAGGCA-1 pbmc3k 3471 1120 1.8438490 4 4
GCAGATACAGCGTT-1 pbmc3k 2092 791 3.7284895 4 4
GCAGATACGACGGA-1 pbmc3k 1670 619 1.6167665 0 0
GCAGATACGCAGAG-1 pbmc3k 2140 750 2.1962617 0 0
GCAGCCGACAGTCA-1 pbmc3k 3034 910 2.8015821 0 0
GCAGCGTGCACTCC-1 pbmc3k 2298 775 1.2619669 0 0
GCAGCTCTCAATCG-1 pbmc3k 1858 659 1.1840689 0 0
GCAGCTCTGTTTCT-1 pbmc3k 1684 762 1.1876485 2 2
GCAGGGCTAAGAAC-1 pbmc3k 2968 767 1.9541779 0 0
GCAGGGCTAAGGGC-1 pbmc3k 1464 673 1.5710383 0 0
GCAGGGCTATCGAC-1 pbmc3k 1980 943 2.3232323 6 6
GCAGGGCTTGGGAG-1 pbmc3k 2194 939 4.9225160 4 4
GCAGTCCTAACTGC-1 pbmc3k 2107 761 2.1357380 2 2
GCAGTCCTCTCTTA-1 pbmc3k 1830 725 2.0218579 0 0
GCATCAGATGCGTA-1 pbmc3k 2047 767 3.8593063 4 4
GCATGTGACAAGCT-1 pbmc3k 2949 871 2.2380468 0 0
GCATTGGAGAAGGC-1 pbmc3k 3069 1043 1.2056044 2 2
GCCAAAACGAGGCA-1 pbmc3k 2087 850 4.4561572 4 4
GCCAAATGATCGAC-1 pbmc3k 2886 994 1.9750520 2 2
GCCAACCTACGGTT-1 pbmc3k 2141 680 2.1952359 0 0
GCCAACCTCGCCTT-1 pbmc3k 1770 595 1.1864407 0 0
GCCACGGAGGCGAA-1 pbmc3k 1999 699 1.7508754 1 1
GCCACGGATACTGG-1 pbmc3k 2407 749 2.1188201 0 0
GCCACTACGTCTTT-1 pbmc3k 2096 735 2.2423664 2 2
GCCCAACTACCGAT-1 pbmc3k 2575 887 1.5533981 0 0
GCCCAACTATGGTC-1 pbmc3k 1831 683 1.8569088 4 4
GCCCATACAGCGTT-1 pbmc3k 1957 778 0.4087890 4 4
GCCGACGAACTCTT-1 pbmc3k 2980 984 2.0805369 1 1
GCCGAGTGCGTTGA-1 pbmc3k 3190 988 3.0407524 2 2
GCCGGAACGAACTC-1 pbmc3k 2325 830 2.1505376 4 4
GCCGGAACGTTCTT-1 pbmc3k 1581 809 2.6565465 6 6
GCCGGAACTGCACA-1 pbmc3k 1591 685 4.9025770 4 4
GCCGGAACTTACTC-1 pbmc3k 2019 782 1.0896483 2 2
GCCGTACTACCTGA-1 pbmc3k 2258 642 3.4986714 0 0
GCCGTACTGGCAAG-1 pbmc3k 1696 740 3.6556604 1 1
GCCTACACAGTTCG-1 pbmc3k 2056 959 1.5077821 6 6
GCCTACACCACTGA-1 pbmc3k 2617 906 2.1016431 5 5
GCCTACACCTTGAG-1 pbmc3k 2816 828 0.8522727 0 0
GCCTAGCTACGGAG-1 pbmc3k 1727 618 0.9843660 3 3
GCCTAGCTCTATTC-1 pbmc3k 2418 821 1.6542597 0 0
GCCTAGCTTCTCAT-1 pbmc3k 2154 716 2.1355617 0 0
GCCTAGCTTCTCTA-1 pbmc3k 3409 1208 3.3147551 5 5
GCCTCAACCATGGT-1 pbmc3k 2602 800 1.1145273 0 0
GCCTCAACTCTTTG-1 pbmc3k 5981 1954 1.1202140 2 2
GCCTCATGTCTTAC-1 pbmc3k 2173 749 3.0372757 4 4
GCCTGACTCTCAAG-1 pbmc3k 2001 754 1.4492754 1 1
GCGAAGGAACTCTT-1 pbmc3k 1247 570 2.3255814 0 0
GCGAAGGATGCCAA-1 pbmc3k 2464 827 1.9886364 0 0
GCGAGAGAGGGACA-1 pbmc3k 1881 719 1.1695906 0 0
GCGAGCACTGTCGA-1 pbmc3k 611 334 1.9639935 3 3
GCGAGCACTTGACG-1 pbmc3k 2281 800 2.4989040 0 0
GCGAGCACTTGCTT-1 pbmc3k 3144 1001 3.0534351 1 1
GCGATATGGTACGT-1 pbmc3k 2070 885 1.0144928 0 0
GCGATATGGTGTTG-1 pbmc3k 1762 631 2.4404086 0 0
GCGCACGAAGTCGT-1 pbmc3k 2262 755 2.7851459 0 0
GCGCACGACTTTAC-1 pbmc3k 1686 628 1.5421115 1 1
GCGCATCTAGGTCT-1 pbmc3k 1395 550 1.3620072 0 0
GCGCATCTGGTTAC-1 pbmc3k 2513 979 2.8651015 8 8
GCGCATCTTCGATG-1 pbmc3k 2295 765 1.6557734 0 0
GCGCATCTTGCTCC-1 pbmc3k 1995 835 2.1052632 6 6
GCGCATCTTTCTAC-1 pbmc3k 2507 776 1.7550858 0 0
GCGCGAACGTTCTT-1 pbmc3k 1659 637 1.3863773 3 3
GCGCGATGAACGGG-1 pbmc3k 2632 857 0.9878419 0 0
GCGCGATGGTGCAT-1 pbmc3k 3225 1410 3.2868217 6 6
GCGGAGCTCCTGAA-1 pbmc3k 1951 746 1.5376730 0 0
GCGGCAACCCGATA-1 pbmc3k 2371 778 1.0544074 0 0
GCGGCAACGGAGGT-1 pbmc3k 1319 539 2.8809704 3 3
GCGGCAACTGTCGA-1 pbmc3k 699 365 4.7210300 1 1
GCGTAAACACGGTT-1 pbmc3k 5924 1625 2.1438217 7 7
GCGTAATGCACCAA-1 pbmc3k 3965 1375 2.7490542 5 5
GCGTATGAACACCA-1 pbmc3k 2130 687 2.3004695 0 0
GCGTATGATGAGAA-1 pbmc3k 2731 1000 0.9886488 1 1
GCTACAGAAAGGTA-1 pbmc3k 2450 805 1.1836735 0 0
GCTACAGAATCTTC-1 pbmc3k 648 337 1.8518519 3 3
GCTACCTGAGAAGT-1 pbmc3k 3293 1101 4.4943820 1 1
GCTACCTGATCACG-1 pbmc3k 2168 796 1.0608856 2 2
GCTACGCTAGAATG-1 pbmc3k 2978 964 4.1974480 0 0
GCTACGCTAGCTAC-1 pbmc3k 2110 782 3.8388626 2 2
GCTACGCTCCCTAC-1 pbmc3k 2757 1016 1.3057671 2 2
GCTAGAACAGAGGC-1 pbmc3k 1512 641 1.6534392 3 3
GCTAGAACGGATCT-1 pbmc3k 2698 965 2.1868050 2 2
GCTAGAACTCCCGT-1 pbmc3k 1102 434 1.8148820 0 0
GCTAGATGAGCTCA-1 pbmc3k 2305 826 3.3839479 1 1
GCTAGATGGCGATT-1 pbmc3k 2014 777 2.8301887 4 4
GCTATACTAAGGCG-1 pbmc3k 3086 998 1.4906027 0 0
GCTATACTAGCGTT-1 pbmc3k 1509 601 3.9098741 4 4
GCTATACTCTCTTA-1 pbmc3k 2779 795 1.9791292 0 0
GCTATACTGGACGA-1 pbmc3k 2823 944 2.4087850 2 2
GCTCAAGAACCATG-1 pbmc3k 5552 1674 1.8011527 5 5
GCTCAAGAAGTCAC-1 pbmc3k 3219 1123 2.3609817 1 1
GCTCAGCTGTCTAG-1 pbmc3k 1682 595 1.1296076 0 0
GCTCCATGAGAAGT-1 pbmc3k 2129 994 1.4091123 4 4
GCTCCATGCCGAAT-1 pbmc3k 1953 719 1.0240655 0 0
GCTCGACTCTAGTG-1 pbmc3k 1417 587 2.7522936 1 1
GCTGATGAGGTATC-1 pbmc3k 1907 758 2.6219192 1 1
GCTTAACTACAGTC-1 pbmc3k 916 452 2.9475983 1 1
GCTTAACTACTGGT-1 pbmc3k 1796 778 2.1714922 4 4
GCTTAACTGCTGAT-1 pbmc3k 2605 836 0.4990403 0 0
GCTTAACTTAGACC-1 pbmc3k 1124 515 3.9145907 1 1
GCTTAACTTCAGTG-1 pbmc3k 2709 844 1.6242156 0 0
GGAACACTCACTTT-1 pbmc3k 778 392 1.9280206 1 1
GGAACACTTCAGAC-1 pbmc3k 623 278 2.8892456 8 8
GGAACTACTACTTC-1 pbmc3k 1803 707 2.6067665 2 2
GGAACTTGAAGGTA-1 pbmc3k 3452 1158 2.4913094 1 1
GGAACTTGAGAATG-1 pbmc3k 2301 794 3.2159930 2 2
GGAACTTGCTCCAC-1 pbmc3k 2403 892 1.7894299 1 1
GGAACTTGGGTAGG-1 pbmc3k 1740 585 4.3678161 3 3
GGAAGGACATCGGT-1 pbmc3k 1386 558 0.9379509 1 1
GGAAGGACCACTAG-1 pbmc3k 2052 857 1.1208577 2 2
GGAAGGACGAGGGT-1 pbmc3k 2879 877 3.3692254 2 2
GGAAGGACGCGAAG-1 pbmc3k 2168 857 1.9833948 2 2
GGAAGGTGGCGAGA-1 pbmc3k 1630 839 2.3312883 6 6
GGAATCTGAAGGGC-1 pbmc3k 1733 642 2.1350260 2 2
GGAATCTGAGGAGC-1 pbmc3k 3498 1305 1.2006861 2 2
GGAATCTGCTTAGG-1 pbmc3k 1512 594 2.3148148 3 3
GGAATCTGCTTGTT-1 pbmc3k 3348 1179 4.8685783 1 1
GGAATCTGGGAGGT-1 pbmc3k 2667 908 0.7874016 0 0
GGAATGCTTTCTAC-1 pbmc3k 2252 708 1.5097691 0 0
GGACAGGAAAGGGC-1 pbmc3k 2172 817 3.8213628 4 4
GGACAGGAGTGCTA-1 pbmc3k 2024 814 2.4209486 3 3
GGACAGGATCTCGC-1 pbmc3k 2190 840 1.2328767 2 2
GGACCCGAAGCTAC-1 pbmc3k 3320 1194 1.6265060 1 1
GGACCGTGCTTACT-1 pbmc3k 1554 639 1.0939511 1 1
GGACCGTGGGAACG-1 pbmc3k 4455 1425 2.8058361 7 7
GGACCGTGTAACGC-1 pbmc3k 2912 945 2.2664835 2 2
GGACCTCTGTAAGA-1 pbmc3k 2396 897 3.2554257 1 1
GGACCTCTTTTCTG-1 pbmc3k 2023 851 3.0647553 4 4
GGACGAGAGTGTCA-1 pbmc3k 930 460 2.5806452 1 1
GGACGCTGACGCAT-1 pbmc3k 3046 1096 2.8562049 4 4
GGACGCTGCTAGCA-1 pbmc3k 3128 823 1.9820972 0 0
GGACGCTGTCCTCG-1 pbmc3k 1529 780 1.3734467 6 6
GGAGAGACGTGAGG-1 pbmc3k 2676 931 1.6068759 3 3
GGAGCAGATTCAGG-1 pbmc3k 1323 541 2.7210884 3 3
GGAGCCACCTTCTA-1 pbmc3k 3349 1135 1.5825620 3 3
GGAGCGCTACGCAT-1 pbmc3k 3764 1223 1.4877790 1 1
GGAGCGCTCCGAAT-1 pbmc3k 3243 994 1.1717545 2 2
GGAGGATGCCACCT-1 pbmc3k 1585 572 0.9463722 0 0
GGAGGATGGTTGAC-1 pbmc3k 1790 778 2.0111732 1 1
GGAGGATGTCAGTG-1 pbmc3k 2009 765 2.3394724 1 1
GGAGGCCTCGTTGA-1 pbmc3k 1941 906 1.5971149 6 6
GGAGGCCTTTCTTG-1 pbmc3k 2407 792 2.1188201 0 0
GGAGGTGATACGCA-1 pbmc3k 2326 753 2.1926053 2 2
GGAGGTGATCGCTC-1 pbmc3k 1811 770 1.9326339 2 2
GGATACTGCAGCTA-1 pbmc3k 2650 852 1.7358491 2 2
GGATACTGTCTAGG-1 pbmc3k 2713 934 1.3638039 2 2
GGATAGCTCGTCTC-1 pbmc3k 3052 1046 1.8348624 2 2
GGATAGCTCTGAAC-1 pbmc3k 2051 817 3.1204291 4 4
GGATGTACCAAAGA-1 pbmc3k 1886 644 1.6436903 3 3
GGATGTACGCGAAG-1 pbmc3k 1594 618 1.5683814 0 0
GGATGTACGTCTTT-1 pbmc3k 562 314 3.0249110 1 1
GGATGTACGTGTCA-1 pbmc3k 1635 723 3.1804281 4 4
GGATTTCTAGGTTC-1 pbmc3k 1900 735 2.3157895 2 2
GGATTTCTTTGTCT-1 pbmc3k 3343 1160 1.9443614 1 1
GGCAAGGAAAAAGC-1 pbmc3k 2374 1078 2.1482730 6 6
GGCAAGGAAGAAGT-1 pbmc3k 2710 867 1.7712177 2 2
GGCAAGGACTTGGA-1 pbmc3k 3092 1030 2.2315653 1 1
GGCAAGGAGGACTT-1 pbmc3k 2909 913 1.5812994 0 0
GGCAATACGCTAAC-1 pbmc3k 1076 474 3.2527881 3 3
GGCAATACGGCATT-1 pbmc3k 1107 488 2.4390244 4 4
GGCAATACGTTTCT-1 pbmc3k 2075 795 2.2168675 3 3
GGCACGTGGCTTAG-1 pbmc3k 3599 1200 1.3337038 5 5
GGCACTCTTTTGTC-1 pbmc3k 2542 994 1.1801731 2 2
GGCATATGCTTATC-1 pbmc3k 1939 897 1.4956163 6 6
GGCATATGGGGAGT-1 pbmc3k 575 212 1.2173913 8 8
GGCATATGTGTGAC-1 pbmc3k 5303 1584 2.7154441 2 2
GGCCACGACAGAGG-1 pbmc3k 890 426 3.0337079 0 0
GGCCAGACTGGTTG-1 pbmc3k 2562 945 1.6783763 2 2
GGCCCAGAAAGTAG-1 pbmc3k 2684 935 1.0804769 1 1
GGCCGAACAACGAA-1 pbmc3k 2353 723 2.5924352 0 0
GGCCGAACGCAGAG-1 pbmc3k 837 380 3.5842294 0 0
GGCCGAACGTAGGG-1 pbmc3k 1808 714 2.1017699 0 0
GGCCGAACTCTAGG-1 pbmc3k 1494 739 0.6024096 6 6
GGCCGATGCAGGAG-1 pbmc3k 2877 930 1.4250956 2 2
GGCCGATGCCGAAT-1 pbmc3k 2614 971 1.3389441 2 2
GGCCGATGTACTCT-1 pbmc3k 2606 771 2.4174981 4 4
GGCGACACTGCCCT-1 pbmc3k 4438 1494 1.7575484 4 4
GGCGACTGCGTAAC-1 pbmc3k 2522 904 0.7137193 2 2
GGCGCATGCCTAAG-1 pbmc3k 2095 797 2.0525060 5 5
GGCGCATGCTCCAC-1 pbmc3k 2534 952 0.7103394 2 2
GGCGCATGTGGAAA-1 pbmc3k 1200 470 0.6666667 0 0
GGCGGACTAGAGGC-1 pbmc3k 1759 820 1.7055145 2 2
GGCGGACTAGGAGC-1 pbmc3k 1974 805 1.6717325 4 4
GGCGGACTCTGACA-1 pbmc3k 2075 713 1.4939759 0 0
GGCGGACTCTTGGA-1 pbmc3k 1933 827 3.3626487 4 4
GGCGGACTTACTGG-1 pbmc3k 2403 726 3.1627133 0 0
GGCGGACTTGAACC-1 pbmc3k 2110 771 1.6113744 2 2
GGCTAAACACCTGA-1 pbmc3k 2433 975 0.5343198 6 6
GGCTAAACTCTTAC-1 pbmc3k 2665 854 1.6885553 0 0
GGCTAATGAGCACT-1 pbmc3k 1650 727 1.8787879 4 4
GGCTAATGGTCTAG-1 pbmc3k 2253 759 2.0861074 0 0
GGCTCACTACTCAG-1 pbmc3k 3515 1083 1.9061166 1 1
GGGAACGAAGCTCA-1 pbmc3k 4319 1554 2.5005788 5 5
GGGAACGACACAAC-1 pbmc3k 1711 691 1.8702513 3 3
GGGAACGAGTGTCA-1 pbmc3k 1148 558 2.2648084 1 1
GGGAAGTGTTGAGC-1 pbmc3k 1553 625 4.3142305 1 1
GGGACCACACGTTG-1 pbmc3k 1863 795 2.6301664 4 4
GGGACCACAGAACA-1 pbmc3k 2090 749 2.3444976 0 0
GGGACCACGAATAG-1 pbmc3k 2384 837 1.2583893 2 2
GGGACCACGTCATG-1 pbmc3k 2558 797 2.0719312 0 0
GGGACCACTCAAGC-1 pbmc3k 1064 453 1.6917293 0 0
GGGACCACTCGTGA-1 pbmc3k 2290 733 2.2707424 0 0
GGGACCACTGCATG-1 pbmc3k 1929 604 1.7107309 0 0
GGGACCTGACCCTC-1 pbmc3k 3079 1028 1.7538162 1 1
GGGACCTGCTTGCC-1 pbmc3k 2338 803 1.9674936 0 0
GGGACCTGTGGAGG-1 pbmc3k 1762 669 3.2349603 3 3
GGGATGGACGACAT-1 pbmc3k 1307 503 1.0711553 3 3
GGGATGGATACTTC-1 pbmc3k 2272 858 2.7728873 2 2
GGGATGGATGGTTG-1 pbmc3k 2068 832 2.3694391 1 1
GGGATTACGTCTAG-1 pbmc3k 3000 1018 1.8666667 0 0
GGGCAAGATGCATG-1 pbmc3k 1424 585 2.3876404 1 1
GGGCACACGGTGAG-1 pbmc3k 4254 1221 3.4555712 5 5
GGGCACACGTTGCA-1 pbmc3k 4081 1286 2.0828228 2 2
GGGCAGCTTGGGAG-1 pbmc3k 2245 902 2.4498886 4 4
GGGCAGCTTTTCTG-1 pbmc3k 2333 752 1.5859408 0 0
GGGCCAACCTTGGA-1 pbmc3k 8415 2013 1.0576352 7 7
GGGCCAACGCGTTA-1 pbmc3k 2177 773 2.5723473 3 3
GGGCCAACTACGCA-1 pbmc3k 1432 663 2.5837989 1 1
GGGCCAACTCCAAG-1 pbmc3k 2437 840 1.8465326 3 3
GGGCCATGATGGTC-1 pbmc3k 2480 851 3.3467742 4 4
GGGCCATGTTGACG-1 pbmc3k 2262 887 1.6357206 2 2
GGGTAACTCAGCTA-1 pbmc3k 3427 1079 2.0717829 2 2
GGGTAACTCTAGTG-1 pbmc3k 1770 653 1.5254237 3 3
GGGTAACTCTGGAT-1 pbmc3k 1948 674 1.9507187 0 0
GGGTTAACGTGCAT-1 pbmc3k 1812 908 1.6556291 6 6
GGTAAAGAGCTAAC-1 pbmc3k 1614 734 3.1598513 4 4
GGTACAACTGCAAC-1 pbmc3k 2654 909 0.6782216 2 2
GGTACATGAAAGCA-1 pbmc3k 1697 670 2.1803182 3 3
GGTACATGAGCTCA-1 pbmc3k 1751 800 0.9137636 2 2
GGTACATGCGGTAT-1 pbmc3k 1373 539 1.7479971 0 0
GGTACATGGTTACG-1 pbmc3k 1928 697 1.4522822 2 2
GGTACATGTGGGAG-1 pbmc3k 2747 941 2.3298143 2 2
GGTACTGAACTCTT-1 pbmc3k 2048 826 2.8320312 1 1
GGTAGTACACCACA-1 pbmc3k 2290 800 2.6200873 4 4
GGTAGTACACTAGC-1 pbmc3k 1655 642 3.0211480 1 1
GGTAGTACCCTGTC-1 pbmc3k 2091 733 1.9607843 0 0
GGTAGTACGCCATA-1 pbmc3k 1345 605 1.5613383 1 1
GGTAGTACTGTCTT-1 pbmc3k 2747 991 1.2377139 2 2
GGTATCGAGACAAA-1 pbmc3k 809 379 1.8541409 1 1
GGTATCGATGAACC-1 pbmc3k 1507 561 2.0570670 3 3
GGTCAAACCAAAGA-1 pbmc3k 2389 844 1.1301800 0 0
GGTCTAGAGAAACA-1 pbmc3k 2333 787 2.8289756 1 1
GGTCTAGATAGCGT-1 pbmc3k 4660 1460 1.2017167 1 1
GGTGATACCGACTA-1 pbmc3k 1694 728 2.4793388 3 3
GGTGATACGACTAC-1 pbmc3k 2716 940 1.4359352 1 1
GGTGATACTGTTTC-1 pbmc3k 2082 783 1.2007685 0 0
GGTGGAGAAACGGG-1 pbmc3k 1394 693 1.7216643 4 4
GGTGGAGAAGTAGA-1 pbmc3k 1897 794 1.6868740 2 2
GGTGGAGACAGATC-1 pbmc3k 1440 809 2.0138889 4 4
GGTGGAGATCGATG-1 pbmc3k 2296 827 2.0905923 2 2
GGTGGAGATCTCTA-1 pbmc3k 1832 647 2.4563319 1 1
GGTGGAGATTACTC-1 pbmc3k 2452 880 1.0195759 4 4
GGTTTACTACGCAT-1 pbmc3k 2344 970 2.2610922 4 4
GTAACGTGACCTCC-1 pbmc3k 4521 1321 1.7916390 1 1
GTAACGTGATCGGT-1 pbmc3k 1887 679 2.4377318 3 3
GTAACGTGCAGCTA-1 pbmc3k 2107 772 1.6136687 3 3
GTAACGTGGTTGAC-1 pbmc3k 2304 781 2.0833333 3 3
GTAAGCACAACGGG-1 pbmc3k 2030 766 0.8374384 0 0
GTAAGCACTCATTC-1 pbmc3k 1137 519 2.1108179 3 3
GTAAGCTGGTACCA-1 pbmc3k 1449 670 2.8985507 1 1
GTAATAACCTTCTA-1 pbmc3k 2207 795 3.5795197 1 1
GTAATAACGTTGTG-1 pbmc3k 2117 643 2.5507794 0 0
GTACCCTGACAGTC-1 pbmc3k 5049 1548 1.6042781 5 5
GTACCCTGGAGCTT-1 pbmc3k 2350 756 1.6595745 0 0
GTACCCTGTCCTTA-1 pbmc3k 1631 723 1.8393624 4 4
GTACCCTGTGAACC-1 pbmc3k 2510 787 2.4701195 0 0
GTACGTGAACGTTG-1 pbmc3k 1741 642 2.8144744 4 4
GTACTTTGTCGACA-1 pbmc3k 2420 856 2.6033058 4 4
GTAGACTGAGATGA-1 pbmc3k 2139 825 2.8050491 1 1
GTAGACTGTATTCC-1 pbmc3k 2008 730 1.0956175 0 0
GTAGCAACAGTCGT-1 pbmc3k 2231 830 1.9722098 1 1
GTAGCAACCATTTC-1 pbmc3k 1655 651 3.3836858 3 3
GTAGCAACGGTAGG-1 pbmc3k 955 429 0.4188482 1 1
GTAGCATGCACTCC-1 pbmc3k 1680 641 2.4404762 3 3
GTAGCATGTAAGCC-1 pbmc3k 1542 635 4.1504540 1 1
GTAGCCCTGACGTT-1 pbmc3k 2451 810 3.2231742 0 0
GTAGCTGAAGCTAC-1 pbmc3k 5102 1526 2.7244218 5 5
GTAGCTGAATTCGG-1 pbmc3k 2429 858 2.2231371 0 0
GTAGGTACACGGGA-1 pbmc3k 2857 971 1.7150858 1 1
GTAGTGACCTCATT-1 pbmc3k 2327 743 1.7619252 0 0
GTAGTGTGAGCGGA-1 pbmc3k 2053 866 1.5586946 6 6
GTAGTGTGAGGCGA-1 pbmc3k 1146 506 2.3560209 1 1
GTAGTGTGTGGTTG-1 pbmc3k 1968 623 0.8638211 0 0
GTATCACTGGTAGG-1 pbmc3k 2502 922 2.7577938 1 1
GTATCTACAGAAGT-1 pbmc3k 2282 974 1.9719544 4 4
GTATCTACGACGAG-1 pbmc3k 1938 800 1.7027864 4 4
GTATCTACGTTACG-1 pbmc3k 2807 1013 3.0281439 2 2
GTATTAGAAACAGA-1 pbmc3k 3487 1426 1.4338973 2 2
GTATTAGAGGTCTA-1 pbmc3k 1408 584 1.4204545 0 0
GTATTCACACAGCT-1 pbmc3k 1804 690 2.9933481 0 0
GTATTCACGGGTGA-1 pbmc3k 1739 702 2.1276596 4 4
GTCAACGACACTGA-1 pbmc3k 3628 1175 1.7916207 2 2
GTCAACGAGTGTAC-1 pbmc3k 2148 806 1.8621974 1 1
GTCAATCTACACCA-1 pbmc3k 2272 882 2.4207746 4 4
GTCAATCTGTAGCT-1 pbmc3k 3332 1245 3.2112845 4 4
GTCAATCTTGTGGT-1 pbmc3k 2260 764 0.6637168 0 0
GTCACCTGCCTCCA-1 pbmc3k 2126 742 1.0348071 0 0
GTCACCTGTCCCGT-1 pbmc3k 2794 905 2.4337867 2 2
GTCATACTAATCGC-1 pbmc3k 1607 663 2.9869322 3 3
GTCATACTGCGATT-1 pbmc3k 1456 668 0.6868132 4 4
GTCATACTTCGCCT-1 pbmc3k 979 359 2.9622063 8 8
GTCATACTTTACCT-1 pbmc3k 1859 792 1.8827327 4 4
GTCATACTTTGACG-1 pbmc3k 2734 955 1.1704462 2 2
GTCCAAGAAAAACG-1 pbmc3k 2260 765 1.3274336 0 0
GTCCACTGACCTCC-1 pbmc3k 3330 935 1.1711712 0 0
GTCCACTGGGTACT-1 pbmc3k 4414 1396 2.7865881 5 5
GTCCAGCTACGGGA-1 pbmc3k 2634 942 1.2528474 0 0
GTCCCATGTGGTGT-1 pbmc3k 4744 1467 2.5295110 1 1
GTCGAATGAAGGCG-1 pbmc3k 2119 904 2.2180274 1 1
GTCGACCTGAATGA-1 pbmc3k 1550 812 2.0645161 6 6
GTCGACCTGTTCAG-1 pbmc3k 1832 568 1.9650655 0 0
GTCGCACTTGAGAA-1 pbmc3k 4063 1374 1.6490278 3 3
GTCTAACTGGTCTA-1 pbmc3k 3369 1058 2.8791926 5 5
GTCTAGGAGCTTCC-1 pbmc3k 3650 1211 2.1095890 1 1
GTGAACACACTCTT-1 pbmc3k 2214 693 1.3550136 0 0
GTGAACACTCAGGT-1 pbmc3k 1243 530 0.9654063 0 0
GTGACCCTTAAGCC-1 pbmc3k 1895 675 1.5303430 2 2
GTGATGACAAGTGA-1 pbmc3k 6329 1815 2.1172381 2 2
GTGATGACCTGAGT-1 pbmc3k 1095 502 2.0091324 3 3
GTGATGACGGTTTG-1 pbmc3k 2084 801 2.4472169 4 4
GTGATTCTCATTTC-1 pbmc3k 2567 941 0.9349435 2 2
GTGATTCTCTCTCG-1 pbmc3k 2595 872 2.6589595 1 1
GTGATTCTGGTTCA-1 pbmc3k 1653 810 1.6938899 6 6
GTGATTCTGTCGAT-1 pbmc3k 1991 694 1.8081366 0 0
GTGATTCTTAGCGT-1 pbmc3k 1343 493 2.4571854 3 3
GTGCCACTCAGGAG-1 pbmc3k 1895 658 0.8970976 0 0
GTGGATTGCACTAG-1 pbmc3k 4560 1466 3.2236842 5 5
GTGGATTGCGGAGA-1 pbmc3k 2773 975 2.2719077 2 2
GTGGATTGTAACGC-1 pbmc3k 2764 832 1.8813314 0 0
GTGTACGATCAGTG-1 pbmc3k 1979 689 1.6675088 0 0
GTGTAGTGGGTACT-1 pbmc3k 2625 919 2.7428571 2 2
GTGTATCTAGCCTA-1 pbmc3k 1432 623 2.4441341 3 3
GTGTATCTAGTAGA-1 pbmc3k 4442 1426 2.0486267 4 4
GTGTATCTGTTACG-1 pbmc3k 2120 815 1.6037736 3 3
GTGTCAGAAGCGTT-1 pbmc3k 2052 905 2.0955166 4 4
GTTAAAACCGAGAG-1 pbmc3k 2019 956 0.8420010 6 6
GTTAAATGCTCGAA-1 pbmc3k 2650 764 2.2641509 0 0
GTTAAATGTCGACA-1 pbmc3k 3778 1202 1.9587083 3 3
GTTAACCTAGCTAC-1 pbmc3k 2447 826 2.6971802 2 2
GTTAACCTTGCTTT-1 pbmc3k 6097 1643 1.6237494 7 7
GTTAGGTGCACTCC-1 pbmc3k 1389 552 3.5277178 0 0
GTTAGGTGCCAGTA-1 pbmc3k 4971 1496 2.6755180 1 1
GTTAGGTGCCCAAA-1 pbmc3k 2189 953 0.5938785 5 5
GTTAGGTGGAACTC-1 pbmc3k 1652 646 1.7554479 3 3
GTTAGTCTAAGAAC-1 pbmc3k 1153 536 4.3365134 4 4
GTTATAGAGGACAG-1 pbmc3k 2052 785 1.6081871 0 0
GTTATGCTTTCATC-1 pbmc3k 2427 810 1.8953440 0 0
GTTCAACTGGGACA-1 pbmc3k 1501 759 2.1319121 6 6
GTTCAACTTATGCG-1 pbmc3k 2656 861 2.2213855 0 0
GTTGACGAGCCCTT-1 pbmc3k 1898 837 0.9483667 4 4
GTTGACGATATCGG-1 pbmc3k 2262 1100 1.9451813 6 6
GTTGAGTGGTCTTT-1 pbmc3k 4394 1389 1.0696404 1 1
GTTGAGTGTGCTTT-1 pbmc3k 3840 1042 1.9531250 2 2
GTTGATCTGGGACA-1 pbmc3k 943 371 0.4241782 0 0
GTTGATCTTTTCAC-1 pbmc3k 2460 970 2.1544715 4 4
GTTGGATGTTTACC-1 pbmc3k 4436 1362 2.5247971 5 5
GTTGTACTATTCCT-1 pbmc3k 3101 1135 1.9671074 1 1
GTTGTACTTTTGGG-1 pbmc3k 2402 845 2.2481266 0 0
GTTTAAGACCATGA-1 pbmc3k 2598 935 2.2709777 1 1
GTTTAAGACTGTCC-1 pbmc3k 1584 710 2.2095960 4 4
TAAACAACCAACCA-1 pbmc3k 3425 1070 1.8686131 2 2
TAAACAACGAATCC-1 pbmc3k 2815 911 1.0657194 3 3
TAAAGACTCAGGAG-1 pbmc3k 2373 853 1.5170670 2 2
TAAATCGATGAGGG-1 pbmc3k 2035 758 1.9164619 0 0
TAACAATGTGCCCT-1 pbmc3k 1343 654 1.5636634 3 3
TAACACCTTCGCTC-1 pbmc3k 2754 980 1.4524328 1 1
TAACACCTTCGTAG-1 pbmc3k 3282 1192 1.7672151 1 1
TAACACCTTGTTTC-1 pbmc3k 999 394 1.1011011 8 8
TAACATGACACTAG-1 pbmc3k 3388 1121 1.9480519 3 3
TAACCGGACTTACT-1 pbmc3k 2009 616 1.3439522 0 0
TAACGTCTCAACCA-1 pbmc3k 1930 746 2.1761658 1 1
TAACGTCTCATTGG-1 pbmc3k 2567 876 2.1425789 0 0
TAACTAGAATTTCC-1 pbmc3k 2537 948 1.4189988 2 2
TAACTAGACTTAGG-1 pbmc3k 2921 965 2.0198562 2 2
TAACTAGATCTGGA-1 pbmc3k 2348 789 1.4480409 0 0
TAACTCACGAGGAC-1 pbmc3k 1920 750 4.0625000 1 1
TAACTCACGTACAC-1 pbmc3k 2011 717 2.6355047 3 3
TAACTCACGTATCG-1 pbmc3k 3806 1197 1.9442985 1 1
TAACTCACTCTACT-1 pbmc3k 2223 948 3.0139451 6 6
TAAGAACTGTGTCA-1 pbmc3k 1729 624 3.7593985 1 1
TAAGAGGACTAAGC-1 pbmc3k 2017 782 2.1318790 3 3
TAAGAGGACTTGTT-1 pbmc3k 2060 913 1.5533981 6 6
TAAGATACGGTTCA-1 pbmc3k 1465 659 0.9556314 1 1
TAAGATTGCGTAGT-1 pbmc3k 2788 912 1.5781923 2 2
TAAGATTGTTGCTT-1 pbmc3k 2289 863 1.2669288 2 2
TAAGCGTGAGGTTC-1 pbmc3k 2837 958 2.2911526 1 1
TAAGCGTGGACAAA-1 pbmc3k 5189 1634 2.0620543 1 1
TAAGCGTGGGAAAT-1 pbmc3k 2329 795 1.1592958 0 0
TAAGCGTGTGCTCC-1 pbmc3k 4972 1502 2.2325020 5 5
TAAGGCTGCCATGA-1 pbmc3k 2360 905 2.5000000 2 2
TAAGGCTGCTGCTC-1 pbmc3k 2146 713 1.5843430 0 0
TAAGGCTGTCTCGC-1 pbmc3k 3420 1195 2.1345029 1 1
TAAGGGCTGCTGTA-1 pbmc3k 1852 867 1.3498920 2 2
TAAGGGCTTTACTC-1 pbmc3k 2206 817 3.2184950 0 0
TAAGTAACCGAGAG-1 pbmc3k 701 374 2.7104137 3 3
TAAGTAACCTCCAC-1 pbmc3k 1740 656 3.3333333 3 3
TAAGTAACCTGTAG-1 pbmc3k 3395 1045 2.7687776 2 2
TAAGTAACTTGTCT-1 pbmc3k 2112 744 2.9356061 2 2
TAATGATGAGCGGA-1 pbmc3k 2767 865 1.5540296 0 0
TAATGCCTCATGAC-1 pbmc3k 4185 1182 0.9557945 2 2
TAATGCCTCGTCTC-1 pbmc3k 4211 1667 1.9710283 6 6
TAATGTGAAGATGA-1 pbmc3k 1314 539 1.4459665 0 0
TAATGTGACTGCAA-1 pbmc3k 2532 942 2.1721959 1 1
TAATGTGATTACTC-1 pbmc3k 2325 838 2.8387097 2 2
TACAAATGGGTACT-1 pbmc3k 2545 925 2.2003929 2 2
TACAATGAAAACAG-1 pbmc3k 1994 795 1.6048144 1 1
TACAATGACTTAGG-1 pbmc3k 1946 732 1.6957862 2 2
TACAATGATGCTAG-1 pbmc3k 2466 831 3.2846715 4 4
TACACACTCACACA-1 pbmc3k 3128 967 2.3017903 0 0
TACACACTCTTACT-1 pbmc3k 1246 620 3.2102729 2 2
TACATAGAACGCAT-1 pbmc3k 2223 814 3.5537562 4 4
TACATCACACGGGA-1 pbmc3k 1621 604 1.6656385 0 0
TACATCACCTGTTT-1 pbmc3k 4201 1390 3.6895977 5 5
TACATCACGCTAAC-1 pbmc3k 1792 652 1.7857143 3 3
TACATCACTGAACC-1 pbmc3k 4600 1507 1.2826087 5 5
TACCATTGAGGTTC-1 pbmc3k 1531 621 1.5022861 0 0
TACCATTGCGGGAA-1 pbmc3k 2067 715 3.0962748 1 1
TACCATTGGGGATG-1 pbmc3k 1424 546 2.3174157 1 1
TACCATTGTGAGGG-1 pbmc3k 2347 862 3.7068598 1 1
TACCGGCTGTTGGT-1 pbmc3k 2807 900 2.7787674 0 0
TACGAGTGATCTCT-1 pbmc3k 2692 853 1.8573551 0 0
TACGAGTGATGCTG-1 pbmc3k 3687 1141 2.0070518 1 1
TACGAGTGCGGAGA-1 pbmc3k 2264 989 1.7226148 2 2
TACGAGTGGTTGGT-1 pbmc3k 2222 858 1.8451845 2 2
TACGATCTAGTGTC-1 pbmc3k 1421 603 2.7445461 0 0
TACGATCTCACTGA-1 pbmc3k 4200 1285 2.3571429 1 1
TACGATCTTACGAC-1 pbmc3k 1686 701 2.7876631 4 4
TACGCAGAGAATCC-1 pbmc3k 2121 859 0.8015087 4 4
TACGCCACATTCCT-1 pbmc3k 1965 766 0.6106870 2 2
TACGCCACTCCCAC-1 pbmc3k 5286 1618 3.1592887 5 5
TACGCCACTCCGAA-1 pbmc3k 1856 714 2.1012931 3 3
TACGGAACGCGTTA-1 pbmc3k 1379 593 2.2480058 3 3
TACGGCCTGGGACA-1 pbmc3k 1557 783 1.7341040 6 6
TACGTACTACGGAG-1 pbmc3k 5280 1606 1.4962121 2 2
TACGTACTCAGTTG-1 pbmc3k 2993 927 1.6371534 2 2
TACGTACTCCCGTT-1 pbmc3k 1936 739 1.9111570 3 3
TACGTTACAGAAGT-1 pbmc3k 2256 805 1.4184397 2 2
TACGTTACCAAGCT-1 pbmc3k 1701 705 2.5279248 1 1
TACTAAGAAAGGTA-1 pbmc3k 988 491 1.4170040 3 3
TACTAAGAATCACG-1 pbmc3k 1217 513 3.1224322 0 0
TACTAAGATGATGC-1 pbmc3k 1861 765 2.3105857 1 1
TACTAAGATTGCGA-1 pbmc3k 2549 878 1.4907807 2 2
TACTACACGAGAGC-1 pbmc3k 3236 993 0.9270705 2 2
TACTACACTTACCT-1 pbmc3k 750 356 3.7333333 3 3
TACTACTGAACCTG-1 pbmc3k 1830 747 1.6939891 1 1
TACTACTGATGTCG-1 pbmc3k 2304 993 1.5625000 6 6
TACTACTGATTCTC-1 pbmc3k 2383 801 1.3008812 0 0
TACTACTGTATGGC-1 pbmc3k 2843 843 2.8491031 0 0
TACTCAACGGTCTA-1 pbmc3k 3738 1191 1.3911182 1 1
TACTCAACTGCTAG-1 pbmc3k 2240 792 2.9464286 3 3
TACTCCCTCAGTTG-1 pbmc3k 1553 603 1.1590470 0 0
TACTCTGAATCGAC-1 pbmc3k 1983 807 1.8154312 6 6
TACTCTGACGAGTT-1 pbmc3k 2523 881 1.8628617 2 2
TACTCTGATTGACG-1 pbmc3k 1490 680 1.5436242 3 3
TACTGGGATCGATG-1 pbmc3k 3823 1126 1.5694481 0 0
TACTGTTGAAAGCA-1 pbmc3k 1990 791 1.8090452 0 0
TACTGTTGAGGCGA-1 pbmc3k 1700 828 2.7647059 6 6
TACTGTTGCTGAAC-1 pbmc3k 5187 1650 3.2003085 3 3
TACTTGACTCCTCG-1 pbmc3k 1705 786 1.6422287 4 4
TACTTGACTGGTGT-1 pbmc3k 1598 609 1.9399249 3 3
TACTTTCTTTTGGG-1 pbmc3k 1590 663 4.1509434 4 4
TAGAAACTAATCGC-1 pbmc3k 2207 789 1.2233802 0 0
TAGAAACTGCTTCC-1 pbmc3k 2206 848 2.3572076 1 1
TAGAAACTGGGATG-1 pbmc3k 1371 678 1.3129103 4 4
TAGAATTGCGACAT-1 pbmc3k 1741 588 2.1252154 0 0
TAGAATTGTATCGG-1 pbmc3k 3220 928 2.0496894 0 0
TAGACGTGCTTGAG-1 pbmc3k 1986 690 3.3736153 0 0
TAGACGTGTCGCTC-1 pbmc3k 2593 840 1.0026996 0 0
TAGAGCACCTTACT-1 pbmc3k 1442 645 2.0110957 3 3
TAGATTGACTTGTT-1 pbmc3k 1592 620 3.4547739 3 3
TAGATTGAGGCATT-1 pbmc3k 812 426 2.2167488 0 0
TAGCATCTCAGCTA-1 pbmc3k 1699 855 1.2360212 6 6
TAGCATCTCCCTCA-1 pbmc3k 1138 522 2.6362039 0 0
TAGCATCTGCTGTA-1 pbmc3k 3624 1258 2.2350993 5 5
TAGCATCTGGGACA-1 pbmc3k 2067 720 2.0319303 0 0
TAGCATCTTGTCGA-1 pbmc3k 1753 641 1.4831717 0 0
TAGCCCACAAAAGC-1 pbmc3k 3162 995 1.3282732 2 2
TAGCCCACAGCCAT-1 pbmc3k 2261 983 2.2114109 4 4
TAGCCCACAGCTAC-1 pbmc3k 2171 1099 2.9018885 6 6
TAGCCCACCCACAA-1 pbmc3k 1985 606 2.2166247 0 0
TAGCCCTGCGGAGA-1 pbmc3k 2042 702 4.8971596 2 2
TAGCCGCTTACGAC-1 pbmc3k 2793 944 1.2531328 2 2
TAGCCGCTTACTTC-1 pbmc3k 2301 886 1.7383746 4 4
TAGCCGCTTTCCAT-1 pbmc3k 3330 1061 2.2522523 1 1
TAGCTACTGAATAG-1 pbmc3k 1645 659 1.1550152 3 3
TAGCTACTGTAGCT-1 pbmc3k 2915 995 1.2692967 2 2
TAGCTACTTTTGCT-1 pbmc3k 1078 491 2.6901670 4 4
TAGGACTGTGCTGA-1 pbmc3k 1708 753 2.9859485 2 2
TAGGAGCTAAGGCG-1 pbmc3k 2038 676 3.0912659 3 3
TAGGAGCTGAGGGT-1 pbmc3k 2596 837 1.8489985 0 0
TAGGAGCTTGCATG-1 pbmc3k 1161 530 0.7751938 3 3
TAGGCAACCGTCTC-1 pbmc3k 1690 597 1.7159763 3 3
TAGGCATGCTCTCG-1 pbmc3k 3097 1265 1.3561511 7 7
TAGGCATGGCGAGA-1 pbmc3k 2402 920 1.2073272 4 4
TAGGCTGATGCCTC-1 pbmc3k 1639 699 1.4643075 1 1
TAGGGACTGAACTC-1 pbmc3k 1917 826 2.7647366 6 6
TAGGTCGACACTGA-1 pbmc3k 2468 827 2.1880065 0 0
TAGGTCGAGGATCT-1 pbmc3k 1221 543 2.0475020 3 3
TAGGTGACACACTG-1 pbmc3k 1991 815 3.6664992 2 2
TAGGTGACACGTTG-1 pbmc3k 1888 708 3.8665254 3 3
TAGGTGTGTTCTGT-1 pbmc3k 1726 842 3.0127462 2 2
TAGGTTCTGAAGGC-1 pbmc3k 3538 1199 1.8937253 2 2
TAGGTTCTTCTTAC-1 pbmc3k 3244 1124 2.4660912 5 5
TAGGTTCTTGCTGA-1 pbmc3k 3805 1324 1.8134034 1 1
TAGTAAACCTCGCT-1 pbmc3k 2781 849 1.9057893 0 0
TAGTAAACGTCACA-1 pbmc3k 4042 1157 2.1276596 2 2
TAGTAATGAGATCC-1 pbmc3k 1812 705 0.8278146 0 0
TAGTACCTAAGAAC-1 pbmc3k 1663 675 1.8641010 1 1
TAGTATGATCTTAC-1 pbmc3k 1833 709 3.0551009 3 3
TAGTATGATTCTCA-1 pbmc3k 3357 989 2.0256181 2 2
TAGTCTTGGCTGTA-1 pbmc3k 652 270 0.0000000 0 0
TAGTCTTGGGACTT-1 pbmc3k 943 443 1.6967126 0 0
TAGTCTTGTGGAAA-1 pbmc3k 2206 891 3.5358114 4 4
TAGTGGTGAAGTGA-1 pbmc3k 2003 1037 3.5946081 6 6
TAGTTAGAACCACA-1 pbmc3k 1735 830 1.5561960 6 6
TAGTTAGATGAACC-1 pbmc3k 2215 813 1.5349887 0 0
TATAAGACAACAGA-1 pbmc3k 982 437 4.8879837 0 0
TATAAGACAGCTCA-1 pbmc3k 4145 1396 2.1954162 5 5
TATAAGTGACACCA-1 pbmc3k 3898 1339 4.3099025 5 5
TATAAGTGTATCGG-1 pbmc3k 973 480 1.6443988 1 1
TATAAGTGTGGTGT-1 pbmc3k 4913 1517 2.4424995 5 5
TATACAGAACCCTC-1 pbmc3k 1975 703 2.3797468 4 4
TATACAGAAGAACA-1 pbmc3k 1662 622 1.5643803 0 0
TATACAGATCCAGA-1 pbmc3k 2248 822 1.8238434 0 0
TATACCACCTGATG-1 pbmc3k 1971 730 3.3992897 3 3
TATACGCTACCAAC-1 pbmc3k 2143 780 1.4932338 2 2
TATAGATGGACGGA-1 pbmc3k 2087 848 1.4374701 2 2
TATAGATGTTCCGC-1 pbmc3k 3648 1207 3.1524123 5 5
TATCCAACCAGCTA-1 pbmc3k 1074 472 1.4897579 1 1
TATCCAACTCTCTA-1 pbmc3k 1971 836 2.7397260 4 4
TATCGACTACTAGC-1 pbmc3k 1478 643 2.5033829 4 4
TATCGACTCGATAC-1 pbmc3k 3150 1100 2.6031746 5 5
TATCGTACAGATGA-1 pbmc3k 2185 879 2.5171625 4 4
TATCGTACATTCCT-1 pbmc3k 2323 894 2.4106759 1 1
TATCTCGAGAGATA-1 pbmc3k 1173 407 1.3640239 0 0
TATCTGACAGGTTC-1 pbmc3k 1888 832 2.4894068 4 4
TATCTGACTGTTTC-1 pbmc3k 2659 842 1.2786762 2 2
TATCTTCTAAACAG-1 pbmc3k 3050 927 2.0655738 0 0
TATGAATGGAGGAC-1 pbmc3k 2028 915 1.0355030 6 6
TATGAATGTTTGCT-1 pbmc3k 3725 1269 2.5771812 5 5
TATGCGGATAACCG-1 pbmc3k 1373 652 3.3503277 2 2
TATGGGTGCATCAG-1 pbmc3k 3544 1457 1.1851016 6 6
TATGGGTGCTAGCA-1 pbmc3k 2022 839 2.3244313 3 3
TATGGTCTCTACCC-1 pbmc3k 1897 705 2.4775962 0 0
TATGTCACGGAACG-1 pbmc3k 3333 953 2.5502550 3 3
TATGTCACTAACCG-1 pbmc3k 3464 1216 1.8187067 1 1
TATGTCACTTCTCA-1 pbmc3k 1384 608 2.9624277 3 3
TATGTGCTCCGATA-1 pbmc3k 2909 967 1.6500516 1 1
TATGTGCTGGATTC-1 pbmc3k 2276 789 0.8347979 0 0
TATTGCTGAAGAAC-1 pbmc3k 805 382 3.8509317 1 1
TATTGCTGCCGTTC-1 pbmc3k 1288 532 4.8913043 3 3
TATTGCTGTCTGGA-1 pbmc3k 2093 627 2.1022456 0 0
TATTGCTGTGCACA-1 pbmc3k 780 388 3.0769231 0 0
TATTTCCTATTGGC-1 pbmc3k 1638 655 1.8925519 1 1
TATTTCCTGGAGGT-1 pbmc3k 2263 1101 1.8117543 6 6
TATTTCCTGGTGTT-1 pbmc3k 2208 781 0.9057971 0 0
TCAACACTGTTTGG-1 pbmc3k 2764 1230 2.4240232 6 6
TCAAGGACAGCGTT-1 pbmc3k 1738 617 1.4959724 0 0
TCAAGGACATTCTC-1 pbmc3k 1217 588 1.8077239 4 4
TCAAGGACGGTGTT-1 pbmc3k 2070 754 1.0144928 1 1
TCAATCACACTCTT-1 pbmc3k 2134 882 2.7647610 4 4
TCAATCACAGTCGT-1 pbmc3k 2581 907 3.2158078 5 5
TCACAACTATGTGC-1 pbmc3k 1839 661 1.9575856 0 0
TCACAACTTTGCTT-1 pbmc3k 1299 557 3.3872209 1 1
TCACATACACTTTC-1 pbmc3k 2579 946 1.5897635 2 2
TCACATACAGGGTG-1 pbmc3k 1202 490 1.6638935 0 0
TCACCCGAGACGGA-1 pbmc3k 4217 1383 2.1579322 1 1
TCACCGTGCTCGCT-1 pbmc3k 4572 1477 3.4995626 5 5
TCACCTCTACGACT-1 pbmc3k 2130 822 3.6150235 4 4
TCACCTCTTCCAAG-1 pbmc3k 2765 1064 2.5678119 5 5
TCACGAGAGGAGGT-1 pbmc3k 1644 681 1.9464720 3 3
TCACTATGGGGCAA-1 pbmc3k 1429 650 1.8894332 4 4
TCACTATGGTTGTG-1 pbmc3k 808 363 0.6188119 3 3
TCAGACGACGCTAA-1 pbmc3k 2308 823 1.2564991 2 2
TCAGACGACGTTAG-1 pbmc3k 2037 932 1.9145803 6 6
TCAGAGACTCCAGA-1 pbmc3k 2093 611 1.4811276 0 0
TCAGCAGACTCCAC-1 pbmc3k 2638 884 1.0993177 0 0
TCAGCGCTCTAGTG-1 pbmc3k 1778 803 2.6996625 4 4
TCAGCGCTGGATCT-1 pbmc3k 1275 530 2.0392157 3 3
TCAGCGCTGGTATC-1 pbmc3k 2359 860 3.0097499 4 4
TCAGGATGAAGTAG-1 pbmc3k 1617 645 1.2368584 0 0
TCAGGATGCCTTTA-1 pbmc3k 3121 1141 2.5953220 5 5
TCAGTGGAAGATCC-1 pbmc3k 1688 736 1.8957346 4 4
TCAGTTACCTACGA-1 pbmc3k 2573 954 2.6816945 1 1
TCAGTTACTAGAAG-1 pbmc3k 2237 824 1.2516764 0 0
TCATCAACCCGATA-1 pbmc3k 2338 723 2.2241232 0 0
TCATCAACTGTTCT-1 pbmc3k 4047 1169 2.7427724 1 1
TCATCATGCAGTTG-1 pbmc3k 2218 840 1.3525699 2 2
TCATCCCTTACTGG-1 pbmc3k 2833 1005 1.3766325 2 2
TCATTCGATACAGC-1 pbmc3k 4629 1416 2.9812054 5 5
TCCACGTGGAAACA-1 pbmc3k 3198 952 1.5947467 2 2
TCCACTCTACACTG-1 pbmc3k 2047 827 0.6839277 2 2
TCCACTCTGAGCTT-1 pbmc3k 3183 1035 2.5133522 1 1
TCCACTCTTACTTC-1 pbmc3k 1502 632 0.7323569 3 3
TCCATAACAAAGTG-1 pbmc3k 2226 750 2.2461815 0 0
TCCATAACCGTAGT-1 pbmc3k 2093 846 1.0033445 3 3
TCCATAACGATGAA-1 pbmc3k 2553 1031 1.6059538 2 2
TCCATAACTACGCA-1 pbmc3k 2496 858 1.7628205 0 0
TCCATCCTCCCTAC-1 pbmc3k 2010 890 2.1393035 4 4
TCCCACGATCATTC-1 pbmc3k 2636 893 1.0622155 2 2
TCCCATCTCAAAGA-1 pbmc3k 3217 1030 1.9894311 1 1
TCCCGAACACAGTC-1 pbmc3k 1721 912 3.5444509 6 6
TCCCGAACTTCGCC-1 pbmc3k 4177 1383 2.4180034 5 5
TCCCGATGAGATCC-1 pbmc3k 1770 747 2.0903955 2 2
TCCCGATGCCTGAA-1 pbmc3k 810 416 2.0987654 1 1
TCCCTACTCAACTG-1 pbmc3k 1717 703 1.4560280 3 3
TCCGAAGACAATCG-1 pbmc3k 1103 522 1.6319130 1 1
TCCGAAGACGTTAG-1 pbmc3k 2865 855 1.7452007 0 0
TCCGGACTGAGGTG-1 pbmc3k 2387 914 0.8797654 2 2
TCCGGACTGTACGT-1 pbmc3k 1940 740 2.6288660 4 4
TCCTAAACATCGAC-1 pbmc3k 893 381 0.7838746 0 0
TCCTAAACCGAGAG-1 pbmc3k 3318 1076 2.6522001 2 2
TCCTAAACCGCATA-1 pbmc3k 1384 669 2.1676301 6 6
TCCTAATGGTTTGG-1 pbmc3k 2659 1100 1.6923656 4 4
TCCTACCTGTCGTA-1 pbmc3k 2507 857 1.0370961 2 2
TCCTATGAAAAGCA-1 pbmc3k 1544 662 2.2020725 4 4
TCGAATCTCTGGTA-1 pbmc3k 1305 581 2.3754789 4 4
TCGACCTGCCGATA-1 pbmc3k 2173 845 1.4726185 5 5
TCGACGCTTCTATC-1 pbmc3k 2540 880 1.0236220 1 1
TCGACGCTTTGACG-1 pbmc3k 2194 821 3.1905196 2 2
TCGAGAACGACAGG-1 pbmc3k 2494 833 4.3303929 2 2
TCGAGAACGTTAGC-1 pbmc3k 3383 1289 1.5962164 2 2
TCGAGCCTATCAGC-1 pbmc3k 1487 596 2.2192334 3 3
TCGAGCCTGCGAGA-1 pbmc3k 3235 1128 2.4420402 4 4
TCGAGCCTTGTGAC-1 pbmc3k 2607 792 3.3371692 0 0
TCGATACTATTCCT-1 pbmc3k 2484 836 1.3687601 4 4
TCGATACTTGCACA-1 pbmc3k 1825 765 2.1369863 2 2
TCGATTTGATGCCA-1 pbmc3k 2115 767 1.1820331 1 1
TCGATTTGCACTCC-1 pbmc3k 2505 814 1.0778443 0 0
TCGATTTGCAGCTA-1 pbmc3k 1685 809 2.3738872 6 6
TCGATTTGCCTACC-1 pbmc3k 2280 911 2.8070175 1 1
TCGATTTGTCGTGA-1 pbmc3k 2734 815 1.8653987 3 3
TCGCAGCTAGATCC-1 pbmc3k 2098 790 2.3832221 4 4
TCGCCATGAGACTC-1 pbmc3k 1862 663 1.7722879 0 0
TCGCCATGTGGTCA-1 pbmc3k 1581 702 1.7710310 3 3
TCGGACCTAACAGA-1 pbmc3k 2908 873 1.1348006 0 0
TCGGACCTATAAGG-1 pbmc3k 1368 657 1.1695906 3 3
TCGGACCTGTACAC-1 pbmc3k 5599 1566 1.4288266 2 2
TCGGTAGAGTAGGG-1 pbmc3k 2104 688 1.5209125 0 0
TCGGTAGATCCCAC-1 pbmc3k 2176 748 1.7463235 3 3
TCGTAGGATCGACA-1 pbmc3k 2904 1075 3.1336088 2 2
TCGTTATGGACAAA-1 pbmc3k 2162 782 1.2488437 2 2
TCTAACACCAGTTG-1 pbmc3k 1086 540 3.6832413 6 6
TCTAACACGAGCAG-1 pbmc3k 3176 1094 1.6057935 7 7
TCTAACTGAACCAC-1 pbmc3k 2300 838 2.6521739 2 2
TCTAAGCTAATGCC-1 pbmc3k 2512 806 1.2738854 0 0
TCTAAGCTTAGTCG-1 pbmc3k 1791 789 1.7867113 4 4
TCTAAGCTTCTAGG-1 pbmc3k 3781 1262 1.6926739 5 5
TCTAAGCTTGTTCT-1 pbmc3k 2050 756 2.4878049 1 1
TCTAAGCTTTCGCC-1 pbmc3k 685 367 0.8759124 3 3
TCTACAACGACTAC-1 pbmc3k 2562 1020 2.2638564 1 1
TCTAGACTTAGAAG-1 pbmc3k 1831 727 3.7138176 1 1
TCTAGTTGCACCAA-1 pbmc3k 3302 1100 1.3325257 1 1
TCTATGTGAAGAGT-1 pbmc3k 1129 476 1.6829052 1 1
TCTATGTGAGTCTG-1 pbmc3k 951 488 2.9442692 0 0
TCTCAAACCTAAGC-1 pbmc3k 1477 616 1.6926202 3 3
TCTCTAGAATTTCC-1 pbmc3k 2723 940 1.2118986 2 2
TCTGATACACGTGT-1 pbmc3k 2224 770 2.0683453 0 0
TCTGATACTCGCCT-1 pbmc3k 2448 781 3.3496732 0 0
TCTTACGAACCTGA-1 pbmc3k 2154 845 2.5069638 1 1
TCTTCAGAGCTACA-1 pbmc3k 3624 1302 1.9591611 1 1
TCTTGATGCGGAGA-1 pbmc3k 2273 821 2.1997360 4 4
TGAAATTGGTGAGG-1 pbmc3k 3345 1108 2.4813154 1 1
TGAACCGAAAACGA-1 pbmc3k 2181 748 2.6134801 0 0
TGAACCGACTACTT-1 pbmc3k 2912 1127 1.4423077 5 5
TGAACCGATTCGGA-1 pbmc3k 2869 956 2.0216103 5 5
TGAAGCACTCACGA-1 pbmc3k 1915 740 2.5587467 4 4
TGAAGCTGAACGAA-1 pbmc3k 2511 779 2.1107129 0 0
TGAAGCTGCATGGT-1 pbmc3k 2028 823 1.9230769 3 3
TGAAGCTGCGTAAC-1 pbmc3k 2177 750 2.3886082 0 0
TGAATAACCACTTT-1 pbmc3k 2590 871 1.7374517 0 0
TGAATAACTCCCAC-1 pbmc3k 2140 937 4.5327103 4 4
TGACACGACCTTAT-1 pbmc3k 1510 606 1.9205298 3 3
TGACCAGACAACCA-1 pbmc3k 1799 697 1.1117287 0 0
TGACCAGAGGATTC-1 pbmc3k 1923 705 2.4440978 0 0
TGACCGCTAAAAGC-1 pbmc3k 3020 962 1.4238411 2 2
TGACCGCTCTGCAA-1 pbmc3k 1925 606 1.6103896 0 0
TGACGATGCAAAGA-1 pbmc3k 1632 620 1.7156863 1 1
TGACGCCTGTACCA-1 pbmc3k 5155 1646 2.9679922 2 2
TGACGCCTTTACTC-1 pbmc3k 2608 1088 0.8819018 2 2
TGACTGGAAGAGAT-1 pbmc3k 1442 545 2.2191401 1 1
TGACTGGACCGTAA-1 pbmc3k 1491 667 0.4694836 0 0
TGACTGGACGCAAT-1 pbmc3k 1754 615 1.6533637 0 0
TGACTGGAGGACAG-1 pbmc3k 1848 719 2.1645022 3 3
TGACTGGATTCTCA-1 pbmc3k 4073 1191 1.9641542 2 2
TGACTTACACACCA-1 pbmc3k 3323 1018 0.9027987 2 2
TGACTTACAGTCTG-1 pbmc3k 4135 1290 1.9105200 1 1
TGACTTTGCGCATA-1 pbmc3k 2347 757 1.3634427 0 0
TGACTTTGTTTGTC-1 pbmc3k 2288 854 1.5734266 2 2
TGAGACACAAGGTA-1 pbmc3k 5135 1546 1.3242454 2 2
TGAGACACTCAAGC-1 pbmc3k 1815 742 3.9669421 4 4
TGAGCTGAATGCTG-1 pbmc3k 4164 1371 2.0413064 5 5
TGAGCTGACTGGAT-1 pbmc3k 2132 802 3.2833021 1 1
TGAGCTGATGCTAG-1 pbmc3k 661 337 3.3282905 1 1
TGAGGACTCTCATT-1 pbmc3k 3665 1117 4.0381992 1 1
TGAGGACTTCATTC-1 pbmc3k 3087 1026 1.8464529 2 2
TGAGGTACGAACCT-1 pbmc3k 4752 1521 2.8619529 5 5
TGAGTCGAGTTACG-1 pbmc3k 1375 643 2.4727273 3 3
TGAGTGACTGAGCT-1 pbmc3k 2456 846 1.9136808 0 0
TGATAAACGAATCC-1 pbmc3k 2518 865 2.1445592 2 2
TGATAAACTCCGTC-1 pbmc3k 2067 940 1.7900339 6 6
TGATAAACTTTCAC-1 pbmc3k 1922 751 1.8210198 0 0
TGATACCTCACTAG-1 pbmc3k 1506 583 1.3280212 1 1
TGATACCTGTTGGT-1 pbmc3k 2215 795 1.5349887 0 0
TGATACCTTATGCG-1 pbmc3k 2264 793 2.3409894 0 0
TGATACCTTGAAGA-1 pbmc3k 3061 973 1.2740934 0 0
TGATATGAACCTTT-1 pbmc3k 2957 935 1.7247210 0 0
TGATCACTAGCATC-1 pbmc3k 1110 524 4.5945946 4 4
TGATCACTCTCGCT-1 pbmc3k 1843 659 2.4416712 0 0
TGATCACTTCTACT-1 pbmc3k 1755 606 2.6780627 3 3
TGATCGGACTGACA-1 pbmc3k 4211 1433 3.6333412 5 5
TGATCGGAGGAGCA-1 pbmc3k 1352 562 1.2573964 3 3
TGATCGGATATGCG-1 pbmc3k 1526 696 3.6697248 5 5
TGATTAGACATTGG-1 pbmc3k 2276 925 1.3181019 2 2
TGATTAGATGACTG-1 pbmc3k 1173 515 2.9838022 3 3
TGATTAGATGCTAG-1 pbmc3k 2241 718 1.8295404 0 0
TGATTCACTATGCG-1 pbmc3k 1900 598 2.3157895 0 0
TGATTCACTGTCAG-1 pbmc3k 1732 638 1.5011547 3 3
TGATTCTGCCGAAT-1 pbmc3k 3100 975 1.8064516 2 2
TGATTCTGCTCTTA-1 pbmc3k 2819 1018 1.3834693 2 2
TGCAAGTGAGAACA-1 pbmc3k 2060 813 2.9126214 2 2
TGCAAGTGGGTAGG-1 pbmc3k 1702 669 1.8213866 1 1
TGCAATCTTCAGGT-1 pbmc3k 6316 1902 1.6941102 7 7
TGCACAGACGACAT-1 pbmc3k 2460 1159 2.8048780 6 6
TGCCAAGAGCAGTT-1 pbmc3k 2914 964 1.6129032 5 5
TGCCAAGATCTCTA-1 pbmc3k 3091 933 1.7470074 2 2
TGCCACTGAACGTC-1 pbmc3k 2652 943 0.7541478 0 0
TGCCACTGCGATAC-1 pbmc3k 1948 988 1.2833676 6 6
TGCCAGCTTGGCAT-1 pbmc3k 2183 789 1.3742556 0 0
TGCCCAACAGCAAA-1 pbmc3k 2790 898 1.2186380 0 0
TGCCCAACCGCATA-1 pbmc3k 2799 982 3.2511611 5 5
TGCCGACTCTCCCA-1 pbmc3k 1901 670 2.2093635 3 3
TGCGAAACAGTCAC-1 pbmc3k 2339 856 2.4796922 3 3
TGCGAAACGTTGCA-1 pbmc3k 1417 626 3.2462950 3 3
TGCGATGAACGGTT-1 pbmc3k 1827 690 2.2988506 2 2
TGCGATGACCTCGT-1 pbmc3k 2220 843 2.0270270 0 0
TGCGATGACTAGTG-1 pbmc3k 2844 938 1.8987342 3 3
TGCGATGACTGCTC-1 pbmc3k 2314 795 1.9446845 3 3
TGCGATGACTTGCC-1 pbmc3k 1950 779 1.2820513 3 3
TGCGATGAGTGCTA-1 pbmc3k 1914 820 2.3510972 1 1
TGCGCACTCTTGAG-1 pbmc3k 1056 468 1.7992424 1 1
TGCGTAGAATAAGG-1 pbmc3k 1587 701 2.8355388 1 1
TGCGTAGACGGGAA-1 pbmc3k 1551 629 2.1921341 3 3
TGCGTAGATGGTCA-1 pbmc3k 5486 1557 2.7706890 5 5
TGCTAGGAAACCGT-1 pbmc3k 2221 866 2.4313372 4 4
TGCTAGGATAGTCG-1 pbmc3k 3055 900 1.7348609 0 0
TGCTATACGGTTCA-1 pbmc3k 2120 795 2.5471698 1 1
TGCTATACTGCTGA-1 pbmc3k 2582 1019 2.9434547 4 4
TGCTGAGAGAGCAG-1 pbmc3k 1992 885 3.4136546 6 6
TGCTGAGATTATCC-1 pbmc3k 2752 879 1.4171512 0 0
TGGAAAGACTCTCG-1 pbmc3k 1913 714 2.4568740 3 3
TGGAAAGAGCGATT-1 pbmc3k 2878 1042 1.1466296 2 2
TGGAAAGAGGTCAT-1 pbmc3k 3493 1237 3.5499571 5 5
TGGAAAGATATGGC-1 pbmc3k 1647 565 2.4286582 0 0
TGGAACACAAACAG-1 pbmc3k 1467 776 2.9993183 6 6
TGGAACACGCTAAC-1 pbmc3k 1654 695 2.4788392 4 4
TGGAAGCTCAGATC-1 pbmc3k 1931 725 1.3464526 0 0
TGGACCCTACACTG-1 pbmc3k 695 364 1.8705036 1 1
TGGACCCTCATGGT-1 pbmc3k 1911 810 2.3547881 4 4
TGGACCCTGGTACT-1 pbmc3k 3734 1212 3.1333690 1 1
TGGACTGAGTATGC-1 pbmc3k 3266 1177 1.7146356 2 2
TGGAGACTATCAGC-1 pbmc3k 3291 1130 1.4889091 1 1
TGGAGACTTCAAGC-1 pbmc3k 2014 660 0.9930487 0 0
TGGAGACTTGACCA-1 pbmc3k 2128 829 1.2687970 4 4
TGGAGGGACGGAGA-1 pbmc3k 2106 786 2.3741690 1 1
TGGAGGGAGCTATG-1 pbmc3k 1147 471 1.3949433 0 0
TGGATCGATAAAGG-1 pbmc3k 2568 835 0.9735202 0 0
TGGATGTGACCTAG-1 pbmc3k 1633 563 2.2045315 3 3
TGGATGTGTGAAGA-1 pbmc3k 2632 926 1.8996960 3 3
TGGATTCTCATACG-1 pbmc3k 2451 958 2.5703794 4 4
TGGCAATGCTTGTT-1 pbmc3k 1723 703 2.5536854 3 3
TGGCACCTTCACGA-1 pbmc3k 768 388 2.6041667 0 0
TGGCACCTTCAGTG-1 pbmc3k 1806 840 1.9379845 1 1
TGGGTATGAAGAGT-1 pbmc3k 3114 964 1.4129737 2 2
TGGGTATGCACAAC-1 pbmc3k 3099 1087 1.4198128 2 2
TGGGTATGGTACGT-1 pbmc3k 1137 511 1.8469657 3 3
TGGGTATGTTTGGG-1 pbmc3k 2341 794 1.9649722 1 1
TGGTAGACATGCCA-1 pbmc3k 2963 900 1.5524806 2 2
TGGTAGACCCTCAC-1 pbmc3k 1801 864 2.8317601 6 6
TGGTAGACCTGATG-1 pbmc3k 1696 789 1.1202830 2 2
TGGTAGTGCACTGA-1 pbmc3k 1110 503 1.1711712 1 1
TGGTATCTAAACAG-1 pbmc3k 2095 701 1.8138425 0 0
TGGTATCTCTTCCG-1 pbmc3k 2483 817 2.0539670 0 0
TGGTCAGACCCAAA-1 pbmc3k 1942 790 1.9567456 0 0
TGGTTACTGACGTT-1 pbmc3k 2737 971 0.5845817 1 1
TGGTTACTGTTCTT-1 pbmc3k 2609 904 2.1464163 0 0
TGTAACCTAGAGGC-1 pbmc3k 2765 871 1.6636528 0 0
TGTAACCTTGCCTC-1 pbmc3k 2334 919 2.9991431 1 1
TGTAATGACACAAC-1 pbmc3k 1461 723 1.4373717 6 6
TGTAATGAGGTAAA-1 pbmc3k 1205 628 1.4107884 6 6
TGTACTTGCTCTAT-1 pbmc3k 4907 1479 1.6710821 5 5
TGTAGGTGCGAGAG-1 pbmc3k 1142 531 3.1523643 3 3
TGTAGGTGCTATGG-1 pbmc3k 4232 1099 2.4810964 2 2
TGTAGGTGCTCTAT-1 pbmc3k 6280 1906 2.7229299 7 7
TGTAGGTGTGCTGA-1 pbmc3k 2541 852 2.0857930 0 0
TGTAGTCTTCCAGA-1 pbmc3k 4143 1255 1.3999517 0 0
TGTAGTCTTGCACA-1 pbmc3k 2176 883 3.4926471 4 4
TGTATCTGTTAGGC-1 pbmc3k 1996 817 1.3527054 2 2
TGTATGCTCATGGT-1 pbmc3k 1479 619 3.2454361 3 3
TGTATGCTGTAGGG-1 pbmc3k 1454 590 2.1320495 0 0
TGTATGCTTTCATC-1 pbmc3k 1966 683 2.1871821 0 0
TGTCAGGAATACCG-1 pbmc3k 2763 883 3.1849439 0 0
TGTCAGGAGATGAA-1 pbmc3k 4079 1332 1.9857808 5 5
TGTCTAACCCCTTG-1 pbmc3k 1978 656 2.7805865 0 0
TGTGACGATTCTCA-1 pbmc3k 3097 1226 1.5498870 4 4
TGTGAGACTGTCAG-1 pbmc3k 2665 979 1.5759850 5 5
TGTGAGACTTGAGC-1 pbmc3k 2446 816 2.3712183 2 2
TGTGAGTGACCACA-1 pbmc3k 1583 582 3.1585597 0 0
TGTGAGTGAGTGCT-1 pbmc3k 4108 1368 2.0447907 5 5
TGTGAGTGGAGATA-1 pbmc3k 968 459 0.7231405 4 4
TGTGATCTCTCTAT-1 pbmc3k 1690 638 2.9585799 3 3
TGTGATCTGACACT-1 pbmc3k 1107 470 3.8843722 0 0
TGTGGATGGCCAAT-1 pbmc3k 1942 647 2.6776519 3 3
TGTTAAGACAAAGA-1 pbmc3k 848 414 1.7688679 1 1
TGTTAAGATAAGGA-1 pbmc3k 2172 906 3.3149171 1 1
TGTTACACCGCATA-1 pbmc3k 3410 1147 1.8475073 5 5
TGTTACACGACTAC-1 pbmc3k 1953 732 3.0721966 5 5
TGTTACTGGCTACA-1 pbmc3k 2483 992 3.0205397 4 4
TGTTACTGTAGTCG-1 pbmc3k 1771 800 3.4443817 4 4
TTAACCACCGTAAC-1 pbmc3k 2191 821 2.7384756 0 0
TTAACCACTAAGGA-1 pbmc3k 1197 528 1.9214703 4 4
TTAACCACTCAGAC-1 pbmc3k 1826 735 2.9572837 4 4
TTACACACGTGTTG-1 pbmc3k 2612 805 1.5696784 2 2
TTACACACTCCTAT-1 pbmc3k 2507 815 2.1938572 0 0
TTACCATGAATCGC-1 pbmc3k 2420 888 3.3057851 1 1
TTACCATGGTTGAC-1 pbmc3k 4142 1305 1.0140029 2 2
TTACCATGTGTCTT-1 pbmc3k 1123 588 3.2947462 4 4
TTACCATGTTGTGG-1 pbmc3k 1547 656 1.4221073 3 3
TTACGACTGAGAGC-1 pbmc3k 3287 1153 1.7341040 2 2
TTACGACTTGACAC-1 pbmc3k 1901 712 2.2093635 1 1
TTACGTACGTTCAG-1 pbmc3k 942 321 2.7600849 8 8
TTACTCGAAGAATG-1 pbmc3k 3860 1126 2.5906736 1 1
TTACTCGACGCAAT-1 pbmc3k 5030 1602 2.4850895 7 7
TTACTCGAGGGTGA-1 pbmc3k 2549 879 2.1184778 0 0
TTACTCGATCTACT-1 pbmc3k 2550 1155 1.7254902 6 6
TTAGAATGTGGTGT-1 pbmc3k 1740 642 1.7241379 0 0
TTAGAATGTGTAGC-1 pbmc3k 627 285 3.3492823 0 0
TTAGACCTCCTACC-1 pbmc3k 2485 825 4.3460765 0 0
TTAGACCTCCTTTA-1 pbmc3k 2311 823 1.5144959 1 1
TTAGCTACAACCGT-1 pbmc3k 2100 718 2.0952381 0 0
TTAGCTACTGTCCC-1 pbmc3k 3699 1249 1.8653690 1 1
TTAGCTACTTTCGT-1 pbmc3k 2921 971 4.3135912 2 2
TTAGGGACGCGAAG-1 pbmc3k 2199 852 2.3647112 4 4
TTAGGGTGCTGGAT-1 pbmc3k 2845 915 1.6871705 3 3
TTAGGGTGTCCTGC-1 pbmc3k 2734 1053 3.1089978 1 1
TTAGGTCTACTTTC-1 pbmc3k 1106 515 3.4358047 1 1
TTAGTCACCAGTTG-1 pbmc3k 2674 1017 1.5706806 2 2
TTAGTCTGAAAGCA-1 pbmc3k 2746 952 3.0225783 3 3
TTAGTCTGCCAACA-1 pbmc3k 1733 830 2.4235430 6 6
TTAGTCTGTGCACA-1 pbmc3k 1690 643 4.0236686 2 2
TTATCCGACTAGTG-1 pbmc3k 1901 665 2.4723830 3 3
TTATCCGAGAAAGT-1 pbmc3k 4229 1324 3.1922440 1 1
TTATGAGAGATAAG-1 pbmc3k 1292 546 2.7863777 0 0
TTATGCACGTCACA-1 pbmc3k 1590 636 1.8238994 3 3
TTATGGCTTATGGC-1 pbmc3k 6164 1782 2.2063595 7 7
TTATTCCTATGCTG-1 pbmc3k 3962 1210 2.1958607 1 1
TTATTCCTGGACAG-1 pbmc3k 2513 787 1.3131715 3 3
TTATTCCTGGTACT-1 pbmc3k 2492 804 2.4478331 3 3
TTATTCCTTCGTGA-1 pbmc3k 3203 1100 2.4664377 2 2
TTCAAAGATAAAGG-1 pbmc3k 2384 883 2.3909396 4 4
TTCAACACAACAGA-1 pbmc3k 2429 847 1.1939070 0 0
TTCAACACCCCAAA-1 pbmc3k 2092 786 2.3900574 1 1
TTCAACACGGACGA-1 pbmc3k 2584 1013 2.0123839 5 5
TTCAAGCTAAGAAC-1 pbmc3k 2640 1002 2.6136364 1 1
TTCAAGCTAGATGA-1 pbmc3k 2972 914 2.3553163 2 2
TTCAAGCTGTTGAC-1 pbmc3k 2373 816 1.9806153 2 2
TTCAAGCTTGATGC-1 pbmc3k 2403 827 1.6229713 0 0
TTCAAGCTTTCGCC-1 pbmc3k 2876 880 2.8164117 0 0
TTCACAACCCGTTC-1 pbmc3k 2526 959 0.9501188 1 1
TTCACAACGTCTGA-1 pbmc3k 677 394 0.5908419 5 5
TTCAGACTACCCAA-1 pbmc3k 789 368 3.6755387 0 0
TTCAGACTCTCGAA-1 pbmc3k 2261 784 0.9730208 0 0
TTCAGTACCGACTA-1 pbmc3k 2767 927 1.7347308 0 0
TTCAGTACTCAAGC-1 pbmc3k 5218 1377 2.2614028 2 2
TTCAGTACTCCTAT-1 pbmc3k 1850 711 2.8648649 1 1
TTCAGTTGCCAAGT-1 pbmc3k 2002 732 2.3976024 2 2
TTCAGTTGTCCTTA-1 pbmc3k 1974 751 0.8611955 7 7
TTCAGTTGTCTAGG-1 pbmc3k 1031 464 1.1639185 0 0
TTCAGTTGTCTCGC-1 pbmc3k 1863 624 1.7713366 3 3
TTCATCGAGGTGGA-1 pbmc3k 1913 734 1.4636696 1 1
TTCATGTGTGGTGT-1 pbmc3k 3024 945 1.8518519 2 2
TTCATTCTATGTCG-1 pbmc3k 2625 876 1.4476190 1 1
TTCATTCTTCTCTA-1 pbmc3k 1985 646 3.8287154 0 0
TTCCAAACCTATGG-1 pbmc3k 1054 533 1.2333966 1 1
TTCCAAACCTCCCA-1 pbmc3k 2722 950 1.3225569 3 3
TTCCAAACTCCCAC-1 pbmc3k 792 485 1.6414141 6 6
TTCCAAACTTGACG-1 pbmc3k 1647 712 2.9751063 4 4
TTCCATGACGAGAG-1 pbmc3k 1840 786 3.2608696 1 1
TTCCATGACTGTCC-1 pbmc3k 1048 437 2.9580153 0 0
TTCCCACTTGAGGG-1 pbmc3k 1518 805 2.3056653 6 6
TTCCCACTTGTCTT-1 pbmc3k 3159 1012 1.8043685 1 1
TTCCTAGAAAGTGA-1 pbmc3k 1551 550 0.3868472 0 0
TTCCTAGACTAGTG-1 pbmc3k 1942 840 1.2358393 5 5
TTCGAGGACTCTAT-1 pbmc3k 2744 927 2.2959184 2 2
TTCGAGGAGGGCAA-1 pbmc3k 1816 757 1.6519824 2 2
TTCGAGGATAGAAG-1 pbmc3k 2747 969 2.6938478 5 5
TTCGATTGAGCATC-1 pbmc3k 1909 707 1.5191200 0 0
TTCGGAGAATGCCA-1 pbmc3k 1189 565 2.8595458 1 1
TTCGGAGATGTGCA-1 pbmc3k 2411 880 1.1613438 0 0
TTCGTATGAAAAGC-1 pbmc3k 1990 800 1.6080402 4 4
TTCGTATGGATAGA-1 pbmc3k 1980 870 1.3131313 4 4
TTCGTATGGTCTGA-1 pbmc3k 2256 803 2.8812057 0 0
TTCGTATGTCCTTA-1 pbmc3k 4346 1357 2.0018408 1 1
TTCTACGAACGTAC-1 pbmc3k 2493 865 1.3237064 0 0
TTCTACGAGTTGGT-1 pbmc3k 2589 945 1.2359985 2 2
TTCTAGTGACACGT-1 pbmc3k 3018 1068 1.0271703 2 2
TTCTAGTGCATGAC-1 pbmc3k 2073 819 3.5697057 1 1
TTCTAGTGGAGAGC-1 pbmc3k 1585 814 2.8391167 6 6
TTCTAGTGGTCACA-1 pbmc3k 2832 1058 1.4830508 1 1
TTCTCAGAAGAGAT-1 pbmc3k 2186 843 1.6925892 0 0
TTCTCAGAAGCATC-1 pbmc3k 2497 881 2.1225471 3 3
TTCTCAGATGGAGG-1 pbmc3k 2641 1049 0.7194245 2 2
TTCTGATGGAGACG-1 pbmc3k 2434 1179 1.8898932 6 6
TTCTTACTCTGGAT-1 pbmc3k 1740 717 2.2413793 5 5
TTGAACCTCCTTGC-1 pbmc3k 1745 656 1.4899713 3 3
TTGAATGAACTACG-1 pbmc3k 2286 879 2.4059493 5 5
TTGAATGACTTACT-1 pbmc3k 2102 709 1.6650809 0 0
TTGAATGATCTCAT-1 pbmc3k 2013 752 1.7386985 3 3
TTGACACTCTGTAG-1 pbmc3k 1942 734 1.3388260 0 0
TTGACACTGATAAG-1 pbmc3k 1314 602 2.6636225 3 3
TTGAGGACAGAACA-1 pbmc3k 2955 943 2.9103215 1 1
TTGAGGACTACGCA-1 pbmc3k 6342 1792 2.4440240 7 7
TTGAGGTGGACGGA-1 pbmc3k 2011 712 1.0442566 0 0
TTGCATTGAGCTAC-1 pbmc3k 1990 720 1.7587940 4 4
TTGCATTGCTAAGC-1 pbmc3k 3244 1093 4.9938348 1 1
TTGCATTGTGACTG-1 pbmc3k 2438 810 1.7227235 0 0
TTGCTAACACCAAC-1 pbmc3k 1918 785 2.1376434 4 4
TTGCTAACACGCTA-1 pbmc3k 2573 914 1.9821220 5 5
TTGCTAACCACTCC-1 pbmc3k 1939 652 2.2692109 0 0
TTGCTATGGTACGT-1 pbmc3k 3108 1103 2.0592021 1 1
TTGCTATGGTAGGG-1 pbmc3k 3404 1266 2.4089307 5 5
TTGGAGACCAATCG-1 pbmc3k 4164 1237 1.1767531 2 2
TTGGAGACGCTATG-1 pbmc3k 2662 862 2.4042074 1 1
TTGGAGACTATGGC-1 pbmc3k 1778 738 2.8683915 3 3
TTGGGAACTGAACC-1 pbmc3k 2651 857 2.1124104 0 0
TTGGTACTACTGGT-1 pbmc3k 3265 1063 2.8483920 1 1
TTGGTACTCTTAGG-1 pbmc3k 2926 750 1.7429938 0 0
TTGGTACTGAATCC-1 pbmc3k 1855 615 2.5336927 3 3
TTGGTACTGGATTC-1 pbmc3k 1883 721 1.9118428 3 3
TTGTACACGTTGTG-1 pbmc3k 1536 570 3.7760417 3 3
TTGTACACTTGCAG-1 pbmc3k 2258 859 1.3728964 2 2
TTGTAGCTAGCTCA-1 pbmc3k 2517 931 2.2248709 2 2
TTGTAGCTCTCTTA-1 pbmc3k 2134 805 2.6241799 3 3
TTGTCATGGACGGA-1 pbmc3k 2176 1080 1.8382353 6 6
TTTAGAGATCCTCG-1 pbmc3k 2362 820 1.3547841 3 3
TTTAGCTGATACCG-1 pbmc3k 2754 885 2.2875817 0 0
TTTAGCTGGATACC-1 pbmc3k 2454 850 1.1002445 1 1
TTTAGCTGTACTCT-1 pbmc3k 5671 1560 2.1160289 7 7
TTTAGGCTCCTTTA-1 pbmc3k 1981 800 2.3220596 1 1
TTTATCCTGTTGTG-1 pbmc3k 3679 1155 1.3047024 1 1
TTTCACGAGGTTCA-1 pbmc3k 2036 720 1.3261297 0 0
TTTCAGTGGAAGGC-1 pbmc3k 1780 691 1.5168539 4 4
TTTCAGTGTCACGA-1 pbmc3k 1632 698 3.4313725 3 3
TTTCAGTGTCTATC-1 pbmc3k 937 458 2.4546425 1 1
TTTCAGTGTGCAGT-1 pbmc3k 1321 636 1.8925057 3 3
TTTCCAGAGGTGAG-1 pbmc3k 2187 873 0.6858711 2 2
TTTCGAACACCTGA-1 pbmc3k 4455 1539 1.3019080 7 7
TTTCGAACTCTCAT-1 pbmc3k 3459 1153 2.1104365 1 1
TTTCTACTGAGGCA-1 pbmc3k 3443 1224 0.9294220 3 3
TTTCTACTTCCTCG-1 pbmc3k 1684 622 2.1971496 3 3
TTTGCATGAGAGGC-1 pbmc3k 1022 452 2.0547945 3 3
TTTGCATGCCTCAC-1 pbmc3k 1984 723 0.8064516 0 0 
CellScatter(pbmc, cell1 = "AAACATACAACCAC-1", cell2 = "AAACATTGAGCTAC-1")

DotPlots

DotPlot(pbmc, features = c("MS4A1", "GNLY", "CD3E", "CD14", "FCER1A", "FCGR3A", "LYZ", "PPBP", "CD8A"))

Which plots do you prefer? Discuss.

DoHeatmap() generates an expression heatmap for given cells and features. In this case, we are plotting the top 20 markers (or all markers if less than 20) for each cluster.

pbmc.markers %>%
    group_by(cluster) %>%
    top_n(n = 10, wt = avg_log2FC) -> top10
DoHeatmap(pbmc, features = top10$gene) + NoLegend()

Assigning cell type identity to clusters

Fortunately in the case of this dataset, we can use canonical markers to easily match the unbiased clustering to known cell types:

Cluster ID Markers Cell Type
0 IL7R, CCR7 Naive CD4+ T
1 CD14, LYZ CD14+ Mono
2 IL7R, S100A4 Memory CD4+
3 MS4A1 B
4 CD8A CD8+ T
5 FCGR3A, MS4A7 FCGR3A+ Mono
6 GNLY, NKG7 NK
7 FCER1A, CST3 DC
8 PPBP Platelet 
new.cluster.ids <- c("Naive CD4 T", "CD14+ Mono", "Memory CD4 T", "B", "CD8 T", "FCGR3A+ Mono",
    "NK", "DC", "Platelet")
names(new.cluster.ids) <- levels(pbmc)
pbmc <- RenameIdents(pbmc, new.cluster.ids)
DimPlot(pbmc, reduction = "umap", label = TRUE, pt.size = 0.5) + NoLegend()

saveRDS(pbmc, file = "../output/pbmc3k_final.rds")

Using Monocle For Pseudotime Trajectory

For this workshop, we’ll use the PBMC data object with Monocle for pseudotime trajectory analysis. It’s debatable whether this is a suitable dataset but will suit our needs for demonstration purposes.

This content is based off the Calculating Trajectories with Monocle 3 and Seurat material as well the Monocle3 documentation, combining it with the PBMC dataset from the original Seurat vignette. We recommend reading the Monocle3 documentation for greater understanding of the Monocle package.

Firstly, load Monocle:

library(monocle3)
library(SeuratWrappers)

As the PBMC data has been processed, we can proceed with converting the pbmc Seurat object to a cell_data_set object, which is a class from the Monocle package. The as.cell_data_set function is used from the SeuratWrappers library and is used to convert the Seurat object into a cell_data_set object.

While we have performed the general analysis steps of quality control, scaling and normalization, dimensionality reduction and clustering with Seurat, Monocle is also capable of performing these steps with its own in-built functions. It is often a matter of preference which package to use, depending on what downstream tasks the analyst would like to perform.

We aren’t going to delve deeply into the properties of the cell_data_set object. Just be aware that this is a different way to represent the count assay data and dimensionality reduction data. The functions from the Monocle package expects the scRNA data to be this class and therefore, the Seurat object needs to be converted to this class. It also means that the Seurat functions that we’ve been using will not work with the cell_data_set object.

cds <- as.cell_data_set(pbmc)

While we have previously clustered the pbmc dataset using Seurat, Monocle will also calculate ‘partitions’ - these are superclusters of the Louvain/Leiden communties that are found using a kNN pruning method. The warning message during the conversion notes that Seurat doesn’t calculate partitions and clusters need to be re-calculated using Monocle.

Examine the cds object:

## Inspect the cds object and compare it to the Seurat pbmc object
cds
## class: cell_data_set 
## dim: 13714 2638 
## metadata(0):
## assays(3): counts logcounts scaledata
## rownames(13714): AL627309.1 AP006222.2 ... PNRC2.1 SRSF10.1
## rowData names(0):
## colnames(2638): AAACATACAACCAC-1 AAACATTGAGCTAC-1 ... TTTGCATGAGAGGC-1
##   TTTGCATGCCTCAC-1
## colData names(8): orig.ident nCount_RNA ... ident Size_Factor
## reducedDimNames(2): PCA UMAP
## mainExpName: RNA
## altExpNames(0):

Now re-cluster the cds object:

cds <- cluster_cells(cds)
p1 <- plot_cells(cds, show_trajectory_graph = FALSE)
p2 <- plot_cells(cds, color_cells_by = "partition", show_trajectory_graph = FALSE)
wrap_plots(p1, p2)

We can see that in the first plot, Monocle has identified 3 clusters but all the clusters fall within the same partition. Ideally, partitions should correspond to clusters of cells within the same path of differentiation or cell within the same trajectory.

Source: Haematopoiesis and red blood cells

We can see from this figure of haematopoiesis that our PBMC sample contains a mix of cells from different cell types and are unlikely to be suitable for calculating a pseudotime trajectory. Nonetheless, we’ll demonstrate the steps involved.

Next, we need to run learn_graph to learn the trajectory graph. This function aims to learn how cells transition through a biological program of gene expression changes in an experiment.

cds <- learn_graph(cds)
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plot_cells(cds, label_groups_by_cluster = FALSE, label_leaves = FALSE, label_branch_points = FALSE)

As expected from the partition plot, Monocle thinks all the cells are from the same partition and therefore has plotted a trajectory line that connects all clusters.

Can we fix this?

Monocle currently thinks that all cells belong to the same partition. We might be able to tweak the clustering for a better result. One thing we can think about is that we have a different number of clusters generated by Monocle (3) when Seurat gave us 9.

If we examine the default parameters by ?FindClusters and ?cluster_cells, we might notice that Seurat’s default clustering algorithm is louvain while Monocle’s is leiden. We aren’t going to delve into the details of these algorithms, but we will say, just be aware of the default behavior of your analysis tools and that the choice in algorithm will affect the results of the clustering.

Let’s try clustering with louvain:

cds <- cluster_cells(cds, cluster_method = "louvain")
p1 <- plot_cells(cds, show_trajectory_graph = FALSE)
p2 <- plot_cells(cds, color_cells_by = "partition", show_trajectory_graph = FALSE)
wrap_plots(p1, p2)

The UMAP on the left looks over-clustered and we’d probably need to tweak more parameters to get Monocle to match the Seurat clustering. We don’t necessarily need to do that because our cds object actually still has the meta-data about the seurat clusters stored in it (examine this with head(colData(cds)). However, importantly, our partition plot looks a little more sensible and no longer has lumped all cells into one supercluster.

Let’s re-run the learn_graph step:

cds <- learn_graph(cds)
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plot_cells(cds, color_cells_by = "partition", label_groups_by_cluster = FALSE, label_leaves = FALSE,
    label_branch_points = FALSE)

Monocle now ‘correctly’ builds trajectories that recognizes distinct cell lineages.

We might choose to remove the B-cells and monocytes and focus just on the cluster of CD4T/CD8T cells, as this is the largest group of cells.

selected_ids <- c("Naive CD4 T", "Memory CD4 T", "CD8 T")
tcells_pbmc <- subset(pbmc, idents = selected_ids)  ## subset the PBMC seurat object to tcells
cds <- as.cell_data_set(tcells_pbmc)  ## convert this to cell_data_set
cds <- cluster_cells(cds)  ## Do not run this step with louvain - it might make your R session very unhappy
cds <- learn_graph(cds)
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plot_cells(cds, label_groups_by_cluster = FALSE, label_leaves = FALSE, label_branch_points = FALSE)

The next step is to order cells in pseudotime:

Pseudotime is a measure of how much progress an individual cell has made through a process such as cell differentiation.

In many biological processes, cells do not progress in perfect synchrony. In single-cell expression studies of processes such as cell differentiation, captured cells might be widely distributed in terms of progress. That is, in a population of cells captured at exactly the same time, some cells might be far along, while others might not yet even have begun the process. This asynchrony creates major problems when you want to understand the sequence of regulatory changes that occur as cells transition from one state to the next. Tracking the expression across cells captured at the same time produces a very compressed sense of a gene’s kinetics, and the apparent variability of that gene’s expression will be very high.

By ordering each cell according to its progress along a learned trajectory, Monocle alleviates the problems that arise due to asynchrony. Instead of tracking changes in expression as a function of time, Monocle tracks changes as a function of progress along the trajectory, which we term “pseudotime”. Pseudotime is an abstract unit of progress: it’s simply the distance between a cell and the start of the trajectory, measured along the shortest path. The trajectory’s total length is defined in terms of the total amount of transcriptional change that a cell undergoes as it moves from the starting state to the end state.

Source: Monocle’s documentation

Monocle needs to be told where the ‘beginning’ of the biological process is. There are a variety of ways that this can be determined - the Monocle documentation has a custom function to find the root of the trajectory based on a subset of cells. If the order_cells function is used without providing which cells to use, it will launch an interface in which we can directly select cells we think are at the beginning of the trajectory.

# a helper function to identify the root principal points:
get_earliest_principal_node <- function(cds, cell_type = "Naive CD4 T") {
    cell_ids <- which(colData(cds)[, "ident"] == cell_type)

    closest_vertex <- cds@principal_graph_aux[["UMAP"]]$pr_graph_cell_proj_closest_vertex
    closest_vertex <- as.matrix(closest_vertex[colnames(cds), ])
    root_pr_nodes <- igraph::V(principal_graph(cds)[["UMAP"]])$name[as.numeric(names(which.max(table(closest_vertex[cell_ids,
        ]))))]

    root_pr_nodes
}
cds <- order_cells(cds, root_pr_nodes = get_earliest_principal_node(cds))

We can now plot the trajectory and color cells by pseudotime:

plot_cells(cds, color_cells_by = "pseudotime", label_cell_groups = FALSE, label_leaves = FALSE,
    label_branch_points = FALSE)

plot_cells(cds, color_cells_by = "ident", label_cell_groups = FALSE, label_leaves = FALSE, label_branch_points = FALSE)

Discuss the results of this pseudotime trajectory (remembering this is a bogus example):

Session Info 
sessionInfo()
## R version 4.1.0 (2021-05-18)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 20.04.3 LTS
## 
## Matrix products: default
## BLAS:   /persistent/software/apps/R/4.1.0/lib/R/lib/libRblas.so
## LAPACK: /persistent/software/apps/R/4.1.0/lib/R/lib/libRlapack.so
## 
## locale:
##  [1] LC_CTYPE=en_AU.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_AU.UTF-8        LC_COLLATE=en_AU.UTF-8    
##  [5] LC_MONETARY=en_AU.UTF-8    LC_MESSAGES=en_AU.UTF-8   
##  [7] LC_PAPER=en_AU.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_AU.UTF-8 LC_IDENTIFICATION=C       
## 
## attached base packages:
## [1] stats4    stats     graphics  grDevices utils     datasets  methods  
## [8] base     
## 
## other attached packages:
##  [1] SeuratWrappers_0.3.0        monocle3_1.0.0             
##  [3] SingleCellExperiment_1.16.0 SummarizedExperiment_1.24.0
##  [5] GenomicRanges_1.46.1        GenomeInfoDb_1.30.0        
##  [7] IRanges_2.28.0              S4Vectors_0.32.3           
##  [9] MatrixGenerics_1.6.0        matrixStats_0.61.0         
## [11] Biobase_2.54.0              BiocGenerics_0.40.0        
## [13] patchwork_1.1.1             SeuratObject_4.0.4         
## [15] Seurat_4.0.5                ggplot2_3.3.5              
## [17] dplyr_1.0.7                
## 
## loaded via a namespace (and not attached):
##   [1] plyr_1.8.6             igraph_1.2.9           lazyeval_0.2.2        
##   [4] splines_4.1.0          listenv_0.8.0          scattermore_0.7       
##   [7] digest_0.6.29          htmltools_0.5.2        viridis_0.6.2         
##  [10] fansi_0.5.0            magrittr_2.0.1         tensor_1.5            
##  [13] cluster_2.1.2          ROCR_1.0-11            remotes_2.4.2         
##  [16] limma_3.50.0           globals_0.14.0         R.utils_2.11.0        
##  [19] spatstat.sparse_2.0-0  colorspace_2.0-2       ggrepel_0.9.1         
##  [22] xfun_0.28              crayon_1.4.2           RCurl_1.98-1.5        
##  [25] jsonlite_1.7.2         spatstat.data_2.1-0    survival_3.2-13       
##  [28] zoo_1.8-9              glue_1.5.1             polyclip_1.10-0       
##  [31] gtable_0.3.0           zlibbioc_1.40.0        XVector_0.34.0        
##  [34] leiden_0.3.9           DelayedArray_0.20.0    future.apply_1.8.1    
##  [37] abind_1.4-5            scales_1.1.1           miniUI_0.1.1.1        
##  [40] Rcpp_1.0.7             viridisLite_0.4.0      xtable_1.8-4          
##  [43] reticulate_1.22        spatstat.core_2.3-2    proxy_0.4-26          
##  [46] rsvd_1.0.5             htmlwidgets_1.5.4      httr_1.4.2            
##  [49] RColorBrewer_1.1-2     ellipsis_0.3.2         ica_1.0-2             
##  [52] R.methodsS3_1.8.1      pkgconfig_2.0.3        farver_2.1.0          
##  [55] sass_0.4.0             uwot_0.1.11            deldir_1.0-6          
##  [58] utf8_1.2.2             tidyselect_1.1.1       labeling_0.4.2        
##  [61] rlang_0.4.12           reshape2_1.4.4         later_1.3.0           
##  [64] munsell_0.5.0          tools_4.1.0            generics_0.1.1        
##  [67] ggridges_0.5.3         evaluate_0.14          stringr_1.4.0         
##  [70] fastmap_1.1.0          yaml_2.2.1             goftest_1.2-3         
##  [73] knitr_1.36             fitdistrplus_1.1-6     purrr_0.3.4           
##  [76] RANN_2.6.1             pbapply_1.5-0          future_1.23.0         
##  [79] nlme_3.1-153           mime_0.12              formatR_1.11          
##  [82] R.oo_1.24.0            compiler_4.1.0         plotly_4.10.0         
##  [85] png_0.1-7              spatstat.utils_2.2-0   tibble_3.1.6          
##  [88] bslib_0.3.1            stringi_1.7.6          highr_0.9             
##  [91] RSpectra_0.16-0        lattice_0.20-45        Matrix_1.3-4          
##  [94] vctrs_0.3.8            pillar_1.6.4           lifecycle_1.0.1       
##  [97] BiocManager_1.30.15    spatstat.geom_2.3-0    lmtest_0.9-39         
## [100] jquerylib_0.1.4        RcppAnnoy_0.0.19       data.table_1.14.2     
## [103] cowplot_1.1.1          bitops_1.0-7           irlba_2.3.5           
## [106] httpuv_1.6.3           R6_2.5.1               promises_1.2.0.1      
## [109] KernSmooth_2.23-20     gridExtra_2.3          parallelly_1.29.0     
## [112] codetools_0.2-18       assertthat_0.2.1       MASS_7.3-54           
## [115] leidenbase_0.1.3       withr_2.4.3            sctransform_0.3.2     
## [118] GenomeInfoDbData_1.2.7 mgcv_1.8-38            parallel_4.1.0        
## [121] grid_4.1.0             rpart_4.1-15           tidyr_1.1.4           
## [124] rmarkdown_2.8          Rtsne_0.15             shiny_1.7.1